Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | |||||
|---|---|---|---|---|---|---|---|
| Antibodies | |||||||
| Total OXPHOS | Abcam | Cat#: ab110411; RRID:AB 2756818 | |||||
| CHK1 | Abcam | Cat#: ab40866; RRID:AB 726820 | |||||
| MT-ND1 | Elabscience | Cat#: E-AB-32173; RRID:AB 2881266 | |||||
| MT-CYTB | ProteinTech | Cat#: 55090–1-AP | |||||
| FLAG | Sigma | Cat#: F7425; RRID:AB_439687 | |||||
| Anti-Puromycin | Sigma | Cat#: MABE343; RRID:AB 2566826 | |||||
| AKT2 | Cell Signaling Tech | Cat#: 3063; RRID:AB 2225186 | |||||
| CHK1-S296 | Cell Signaling Tech | Cat#: 2349; RRID:AB 2080323 | |||||
| Anti-biotin, HRP-linked | Cell Signaling Tech | Cat#: 7075; RRID:AB 10696897 | |||||
| YH2AX | Cell Signaling Tech | Cat#: 2577; RRID:AB 2118010 | |||||
| FLAG | Cell Signaling Tech | Cat#: 14793; RRID:AB 2572291 | |||||
| LAMIN A/C | Cell Signaling Tech | Cat#: 4777; RRID:AB 10545756 | |||||
| HA | Cell Signaling Tech | Cat#: 3724; RRID:AB 1549585 | |||||
| TOM20 | Cell Signaling Tech | Cat#: 13929; RRID:AB 2631994 | |||||
| p-ACTIN | Cell Signaling Tech | Cat#: 4970; RRID:AB 2223172 | |||||
| MT-ATP6 | ProteinTech | Cat#: 55313–1-AP; RRID:AB 2881305 | |||||
| SSBP1 | ProteinTech | Cat#:12212–1-AP; RRID:AB 2195320 | |||||
| DNA | Progen | Cat#: 61014; RRID:AB 2750935 | |||||
| CHK1 | Santa Cruz | Cat#: SC-8408; RRID:AB 627257 | |||||
| HRP-labeled anti-mouse | Santa Cruz | Cat#:SC-2005; RRID:AB 631736 | |||||
| HRP-labeled anti-rabbit | Santa Cruz | Cat#: SC-2030; RRID:AB 631747 | |||||
| Anti-mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 594 Conjugate) | Cell Signaling Tech | Cat#: 8890; RRID:AB 2714182 | |||||
| Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor(R) 647 Conjugate) | Cell Signaling Tech | Cat#: 4414; RRID:AB 10693544 | |||||
| Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor(R) 488 Conjugate) | Cell Signaling Tech | Cat#: 4412; RRID:AB 1904025 | |||||
| Bacterial and virus strains | |||||||
| DH5a | Fisher Scientific | Cat#:18265017 | |||||
| Chemicals, peptides, and recombinant proteins | |||||||
| RPMI-160 | Corning | Cat#: ICN1646454 | |||||
| FBS | Corning | Cat#: 35–010-CV | |||||
| DMEM | Corning | Cat#: 10–013-CV | |||||
| L-Glutamine | Corning | Cat#: 25–005-CI | |||||
| Glutamax I | Invitrogen | Cat#: 35050061 | |||||
| Penicillin-Streptomycin | Millipore | Cat#: P0781 | |||||
| Blasticidin | Fisher Scientific | Cat#: 50712728 | |||||
| Puromycin | Sigma-Aldrich | Cat#: P8833 | |||||
| Streptavidin agarose resin | Fisher Scientific | Cat#: 20347 | |||||
| Sequencing grade modified trypsin | Promega | Cat#: V5111 | |||||
| 2-Iodoacetamide | Fisher Scientific g | Cat#: ICN10035125 | |||||
| Iodoacetamide-desthiobiotin | Santa Cruz | Cat#: sc-300424 | |||||
| Nitroso-DTB | This Study | N/A | |||||
| DMSO | Fisher Scientific | Cat#: MT- 25950CQC | |||||
| Urea | VWR Intl | Cat#: 97063–798 | |||||
| CHAPS hydrate | Sigma-Aldrich | Cat#: C3023 | |||||
| DTT | Fisher Scientific | Cat#: BP1725 | |||||
| Micro bio-spin column | Bio-rad | Cat#: 7326204 | |||||
| Protease Inhibitor Cocktail | Sigma-Aldrich | Cat#: 5892791001 | |||||
| PhosSTOP™ | Sigma-Aldrich | Cat#: 4906845001 | |||||
| Polybrene | Santa Cruz | Cat#: 134220 | |||||
| X-tremegene 9 transfection reagent | Sigma-Aldrich | Cat#: 6365787001 | |||||
| X-tremegene HP transfection reagent | Sigma-Aldrich | Cat#: 6366236001 | |||||
| Anti-FLAG M2 Affinity Gel | Sigma-Aldrich | Cat#: A2220 | |||||
| Anti-HA Magnetic Beads | Fisher Scientific | Cat#: 88836 | |||||
| Cell Titer Glo™ reagent | Promega | Cat#: G7570 | |||||
| Prolong Gold Antifade Mount | Life Technologies | Cat#: P36930 | |||||
| Formaldehyde | Sigma | Cat#: UN3334 | |||||
| Arsenic Trioxide | Fisher Scientific | Cat#: R0817000500 | |||||
| β-Lapachone | Cayman Chemical | Cat#: 15021 | |||||
| Doxorubicin | Cayman Chemical | Cat#: 15007 | |||||
| Elesclomol | Selleckchem | Cat#: S1052 | |||||
| Cisplatin | Cayman Chemical | Cat#: 13119 | |||||
| Bleomycin | Cayman Chemical | Cat#: 13877 | |||||
| 5-Fluorouracil | Cayman Chemical | Cat#: 14416 | |||||
| Sulfasalazine | Cayman Chemical | Cat#: 15025 | |||||
| Auranofin | Cayman Chemical | Cat#: 15316 | |||||
| 2-Methoxyestradiol | Cayman Chemical | Cat#: 13021 | |||||
| NOV-002 | MedKoo | Cat#: 202030 | |||||
| Hydrogen peroxide solution | Sigma | Cat#: 216763 | |||||
| Doxycycline | Sigma | Cat#: D3447 | |||||
| MitoTEMPO | Sigma | Cat#: SML0737 | |||||
| N-acetyl-L-Cysteine | Sigma | Cat#: 20261 | |||||
| Carboxy-DCFDA (5-(and-6)-Carboxy-2’,7’- Dichlorofluorescein Diacetate) | Fisher Scientific | Cat#: C369 | |||||
| MitoSOX™ Red | Fisher Scientific | Cat#: M36008 | |||||
| L-ALA | TCI America | Cat#: A0179 | |||||
| D-ALA | TCI America | Cat#: A0177 | |||||
| L-Buthionine-sulfoximine | Sigma | Cat#: B2515 | |||||
| Trolox | Cayman | Cat#: 10011659 | |||||
| Glutathione reduced ethyl ester | Sigma | Cat#: G1404 | |||||
| L -(+)-Ergothioneine | Sigma | Cat#: 497-30-3 | |||||
| S1QEL1.1 | Sigma | Cat#: SML1948 | |||||
| S3QEL-2 | Sigma | Cat#: SML1554 | |||||
| ATP, [Y-32P] | Perkin Elmer | Cat#: BLU002Z250UC | |||||
| Critical commercial assays | |||||||
| NADP/NADPH-Glo™ Assay kit | Promega | Cat#: G9081 | |||||
| NAD/NADH-Glo™ Assay kit | Promega | Cat#: G9071 | |||||
| GSH/GSSG-Glo™ Assay | Promega | Cat#: V6611 | |||||
| Deposited data | |||||||
| Proteomics data | This study | PRIDE: PXD041138 | |||||
| Experimental models: Cell lines | |||||||
| K562 | ATCC | CCL-243 | |||||
| HEK293T | ATCC | CRL-11268 | |||||
| K562-dCas9-KRAB | Gilbert et al., 2014 | N/A | |||||
| OVCAR3 | ATCC | HTB-161 | |||||
| Kuramochi | JCRB | JCRB0098 | |||||
| OVCAR8 | ATCC | CMT-929 | |||||
| PEO1 | ECACC | 10032308 | |||||
| CAOV3 | ATCC | HTB-75 | |||||
| OVISE | JCRB | JCRB1043 | |||||
| OV90 | ATCC | CRL-11732 | |||||
| OVCAR4 | NCI/DTP | N/A | |||||
| OAW28 | ECACC | 85101601 | |||||
| Recombinant DNA | |||||||
| Plenti: Hyper7-NLS | This study | N/A | |||||
| Plenti: Mito-Hyper7 | This study | N/A | |||||
| Plenti: roGFP2-Orp1-NLS | This study | N/A | |||||
| Plenti: Mito-roGFP2-Orp1 | This study | N/A | |||||
| Plenti: FLAG-DAAO-NLS | This study | N/A | |||||
| Plenti: FLAG-MITO-DAAO | This study | N/A | |||||
| Plenti: FLAG-Mito-DAAO-CHK1 | This study | N/A | |||||
| Plenti: CHK1 | This study | N/A | |||||
| pLJM1: FLAG-METAP2 | This study | N/A | |||||
| pLJM1: FLAG-CHK1 | This study | N/A | |||||
| pLJM1: FLAG-CHK1 (C408D) | This study | N/A | |||||
| pLJM1: FLAG-CHK1 (C408S) | This study | N/A | |||||
| pRK5: FLAG-METAP2 | This study | N/A | |||||
| pRK5: FLAG-CHK1 | This study | N/A | |||||
| pRK5: FLAG-CHK1(C408D) | This study | N/A | |||||
| pRK5: FLAG-CHK1-Kinase domain | This study | N/A | |||||
| pRK5: HA-CHK1-KA1 | This study | N/A | |||||
| pRK5: HA-CHK1-KA1 (C408D) | This study | N/A | |||||
| Lenti-CRISPRv2: sgSSBP1–1 | This study | N/A | |||||
| Lenti-CRISPRv2: sgSSBP1–2 | This study | N/A | |||||
| Lenti-CRISPRv2: sgCTRL | This study | N/A | |||||
| Pu6-BFP: sgCONTROL | This study | N/A | |||||
| pU6-BFP: sgSSBP1–1 | This study | N/A | |||||
| pU6-BFP: sgSSBP1–2 | This study | N/A | |||||
| pU6-BFP: sgCHK1–1 | This study | N/A | |||||
| pU6-BFP: sgCHK1–2 | This study | N/A | |||||
| pLKO: shCTRL | This study | N/A | |||||
| pLKO: shSSBP1_1 | This study | N/A | |||||
| pLKO: shSSBP1_2 | This study | N/A | |||||
| Plenti: SSBP1-HA | This study | N/A | |||||
| Plenti: NLS-SSBP1-HA | This study f ^ | N/A | |||||
| Plenti: SSBP1-FLAG | This study | N/A | |||||
| Plenti: SSBP1-shRNA mutant | This study | N/A | |||||
| Plenti: SSBP1 (S67A)-HA | This study | N/A | |||||
| Plenti: SSBP1 (S67D)-HA | This study | N/A | |||||
| Plenti: SSBP1-gRNA mutant | This study | N/A | |||||
| Plenti: SSBP1 (S67A)-gRNA mutant | This study | N/A | |||||
| Plenti: SSBP1 (S67D)-gRNA mutant | This study | N/A | |||||
| Software and algorithms | |||||||
| Prism (v7.0) | GraphPad | https://www.graphpad.com/scientific-software/prism/ | |||||
| FlowJo (v10.0.7) | Treestar Inc. | https://www.flowjo.com/ | |||||
| ZEN 2.6 | Zeiss | https://www.zeiss.com | |||||
| Floview 3.1 | Olympus | https://www.olympus-lifescience.com/ | |||||
| ImageJ | NIH | https://imagej.net/software/fiji/ | |||||