Table 1. Primer sets used to detect gene expression in rats.
Name | Sequence | Reference sequence | |
GAPDH | Forward | 5`-CCATCAACGACCCCTTCATT-3` | NM_017008.4 |
Reverse | 5`-CACGACATACTCAGCACCAGC-3` | ||
Wnt | Forward | 5`-CTGACCTGATGCAGACGCAAG-3` | NM_003391 |
Reverse | 5`-AGGAGCCACCTGTAGCTCTCATGTA-3` | ||
β-catenin | Forward | 5`-TCCGTCGCCGGTCCACACCC-3` | NM_031144.3 |
Reverse | 5`-TCACCAACTGGGACGATATG-3` | ||
E-Cadherin | Forward | 5`-ATCCTGGCCCTCCTGATTCT-3` | NM_031334.1 |
Reverse | 5`-CGGGTATCGTCATCTGGTGG-3` | ||
SMAD4 | Forward | 5`-GGATGAAGTCCTGCACACCA-3` | NM_019275.3 |
Reverse | 5`-GTTGAAGCACTGCCACCTTG-3` |