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. 2023 May 31;15(5):e39789. doi: 10.7759/cureus.39789

Table 1. Primer sets used to detect gene expression in rats.

Name Sequence Reference sequence
GAPDH Forward 5`-CCATCAACGACCCCTTCATT-3` NM_017008.4
Reverse 5`-CACGACATACTCAGCACCAGC-3`
Wnt Forward 5`-CTGACCTGATGCAGACGCAAG-3` NM_003391
Reverse 5`-AGGAGCCACCTGTAGCTCTCATGTA-3`
β-catenin Forward 5`-TCCGTCGCCGGTCCACACCC-3` NM_031144.3
Reverse 5`-TCACCAACTGGGACGATATG-3`
E-Cadherin Forward 5`-ATCCTGGCCCTCCTGATTCT-3` NM_031334.1
Reverse 5`-CGGGTATCGTCATCTGGTGG-3`
SMAD4 Forward 5`-GGATGAAGTCCTGCACACCA-3` NM_019275.3
Reverse 5`-GTTGAAGCACTGCCACCTTG-3`