Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 8;13(9):2863–2878. doi: 10.7150/thno.81240

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

PMC Copyright notice

NOX4 expression modulated ROS production and NF-κB p65 signaling activation in LPS-stimulated TCMK-1 cells. (A) ROS levels in TCMK-1 cells were determined by flow cytometry. (B) Representative confocal images of MitoSOX staining in TCMK-1 cells (×630, scale bars = 20 μm). (C) Representative micrographs of double immunofluorescence staining of NOX4 (green) and p65 (red) in TCMK-1 cells (×400, scale bars = 20 μm). (D) The protein expression levels of p-IκBα, IκBα, p-p65 and p65 in TCMK-1 cells were analyzed by western blot analysis and quantified by densitometry. Data are represented as the mean ± SD, n = 3. ^****P < 0.0001 for LPS + siNC vs siNC, LPS + Ad-Null vs Ad-Null, or LPS vs Ctrl; ^##P < 0.01, ^####P < 0.0001 for LPS + siNOX4 vs LPS + siNC, LPS + Ad-NOX4 vs LPS + Ad-Null, or LPS + GKT vs LPS.