Skip to main content
NCBI home page
British Heart Journal logoLink to British Heart Journal
. 1990 Feb;63(2):88–92. doi: 10.1136/hrt.63.2.88

Lipid peroxidation during myocardial ischaemia induced by pacing.

K G Oldroyd 1, M Chopra 1, A C Rankin 1, J J Belch 1, S M Cobbe 1
PMCID: PMC1024332  PMID: 2317414

Abstract

Oxygen derived free radical generation can be shown in experimental models of myocardial ischaemia and reperfusion and may cause cellular damage by peroxidizing polyunsaturated membrane phospholipids. An attempt was made to quantify human intracardiac lipid peroxidation during transient myocardial ischaemia by measuring the aortic and coronary sinus concentrations of malondialdehyde (a marker of lipid peroxidation) before, during, and after incremental pacing. Twenty six patients were paced until they had severe chest pain or 2 mm ST segment depression or they reached a paced rate of 180 beats/min. They were divided into two groups according to whether or not lactate was produced during pacing. Twelve patients (group 1), all with coronary artery disease, produced myocardial lactate during pacing. None of the other 14 patients (group 2), half of whom had coronary disease, produced lactate during pacing. Concentrations of malondialdehyde in the aorta and coronary sinus were significantly higher in group 1 than in group 2. Five minutes after the end of pacing coronary sinus malondialdehyde concentrations in group 1 had increased significantly from baseline values. There were no changes with time in the coronary sinus concentration of malondialdehyde in group 2 or in the aorta in either group. The negative malondialdehyde extraction ratio in group 1 suggests that intracardiac lipid peroxidation occurs during transient human myocardial ischaemia.

Full text

PDF
88

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambrosio G., Weisfeldt M. L., Jacobus W. E., Flaherty J. T. Evidence for a reversible oxygen radical-mediated component of reperfusion injury: reduction by recombinant human superoxide dismutase administered at the time of reflow. Circulation. 1987 Jan;75(1):282–291. doi: 10.1161/01.cir.75.1.282. [DOI] [PubMed] [Google Scholar]
  2. Aznar J., Santos M. T., Valles J., Sala J. Serum malondialdehyde-like material (MDA-LM) in acute myocardial infarction. J Clin Pathol. 1983 Jun;36(6):712–715. doi: 10.1136/jcp.36.6.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bolli R. Oxygen-derived free radicals and postischemic myocardial dysfunction ("stunned myocardium"). J Am Coll Cardiol. 1988 Jul;12(1):239–249. doi: 10.1016/0735-1097(88)90381-6. [DOI] [PubMed] [Google Scholar]
  4. Bolli R., Patel B. S., Jeroudi M. O., Lai E. K., McCay P. B. Demonstration of free radical generation in "stunned" myocardium of intact dogs with the use of the spin trap alpha-phenyl N-tert-butyl nitrone. J Clin Invest. 1988 Aug;82(2):476–485. doi: 10.1172/JCI113621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burrell C. J., Blake D. R. Reactive oxygen metabolites and the human myocardium. Br Heart J. 1989 Jan;61(1):4–8. doi: 10.1136/hrt.61.1.4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cobbe S. M., Poole-Wilson P. A. Continuous coronary sinus and arterial pH monitoring during pacing-induced ischaemia in coronary artery disease. Br Heart J. 1982 Apr;47(4):369–374. doi: 10.1136/hrt.47.4.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Corr P. B., Gross R. W., Sobel B. E. Amphipathic metabolites and membrane dysfunction in ischemic myocardium. Circ Res. 1984 Aug;55(2):135–154. doi: 10.1161/01.res.55.2.135. [DOI] [PubMed] [Google Scholar]
  8. Cross C. E., Halliwell B., Borish E. T., Pryor W. A., Ames B. N., Saul R. L., McCord J. M., Harman D. Oxygen radicals and human disease. Ann Intern Med. 1987 Oct;107(4):526–545. doi: 10.7326/0003-4819-107-4-526. [DOI] [PubMed] [Google Scholar]
  9. Downey J. M., Hearse D. J., Yellon D. M. The role of xanthine oxidase during myocardial ischemia in several species including man. J Mol Cell Cardiol. 1988 Mar;20 (Suppl 2):55–63. doi: 10.1016/0022-2828(88)90332-x. [DOI] [PubMed] [Google Scholar]
  10. Guarnieri C., Flamigni F., Caldarera C. M. Role of oxygen in the cellular damage induced by re-oxygenation of hypoxic heart. J Mol Cell Cardiol. 1980 Aug;12(8):797–808. doi: 10.1016/0022-2828(80)90081-4. [DOI] [PubMed] [Google Scholar]
  11. Herman M. V., Elliott W. C., Gorlin R. An electrocardiographic, anatomic, and metabolic study of zonal myocardial ischemia in coronary heart disease. Circulation. 1967 May;35(5):834–846. doi: 10.1161/01.cir.35.5.834. [DOI] [PubMed] [Google Scholar]
  12. Homans D. C., Sublett E., Dai X. Z., Bache R. J. Persistence of regional left ventricular dysfunction after exercise-induced myocardial ischemia. J Clin Invest. 1986 Jan;77(1):66–73. doi: 10.1172/JCI112303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jewett S. L., Eddy L. J., Hochstein P. Is the autoxidation of catecholamines involved in ischemia-reperfusion injury? Free Radic Biol Med. 1989;6(2):185–188. doi: 10.1016/0891-5849(89)90116-0. [DOI] [PubMed] [Google Scholar]
  14. Jolly S. R., Kane W. J., Bailie M. B., Abrams G. D., Lucchesi B. R. Canine myocardial reperfusion injury. Its reduction by the combined administration of superoxide dismutase and catalase. Circ Res. 1984 Mar;54(3):277–285. doi: 10.1161/01.res.54.3.277. [DOI] [PubMed] [Google Scholar]
  15. Knight J. A., Pieper R. K., McClellan L. Specificity of the thiobarbituric acid reaction: its use in studies of lipid peroxidation. Clin Chem. 1988 Dec;34(12):2433–2438. [PubMed] [Google Scholar]
  16. Knight J. A., Smith S. E., Kinder V. E., Anstall H. B. Reference intervals for plasma lipoperoxides: age-, sex-, and specimen-related variations. Clin Chem. 1987 Dec;33(12):2289–2291. [PubMed] [Google Scholar]
  17. Liu J., Simon L. M., Phillips J. R., Robin E. D. Superoxide dismutase (SOD) activity in hypoxic mammalian systems. J Appl Physiol Respir Environ Exerc Physiol. 1977 Jan;42(1):107–110. doi: 10.1152/jappl.1977.42.1.107. [DOI] [PubMed] [Google Scholar]
  18. Muxfeldt M., Schaper W. The activity of xanthine oxidase in heart of pigs, guinea pigs, rabbits, rats, and humans. Basic Res Cardiol. 1987 Sep-Oct;82(5):486–492. doi: 10.1007/BF01907096. [DOI] [PubMed] [Google Scholar]
  19. Opie L. H., Owen P., Riemersma R. A. Relative rates of oxidation of glucose and free fatty acids by ischaemic and non-ischaemic myocardium after coronary artery ligation in the dog. Eur J Clin Invest. 1973 Sep;3(5):419–435. doi: 10.1111/j.1365-2362.1973.tb02210.x. [DOI] [PubMed] [Google Scholar]
  20. Parker J. O., West R. O., Case R. B., Chiong M. A. Temporal relationships of myocardial lactate metabolism, left ventricular function, and S-T segment depression during angina precipitate by exercise. Circulation. 1969 Jul;40(1):97–111. doi: 10.1161/01.cir.40.1.97. [DOI] [PubMed] [Google Scholar]
  21. Reimer K. A., Jennings R. B. Failure of the xanthine oxidase inhibitor allopurinol to limit infarct size after ischemia and reperfusion in dogs. Circulation. 1985 May;71(5):1069–1075. doi: 10.1161/01.cir.71.5.1069. [DOI] [PubMed] [Google Scholar]
  22. Rossen R. D., Swain J. L., Michael L. H., Weakley S., Giannini E., Entman M. L. Selective accumulation of the first component of complement and leukocytes in ischemic canine heart muscle. A possible initiator of an extra myocardial mechanism of ischemic injury. Circ Res. 1985 Jul;57(1):119–130. doi: 10.1161/01.res.57.1.119. [DOI] [PubMed] [Google Scholar]
  23. Santos M. T., Valles J., Aznar J., Vilches J. Determination of plasma malondialdehyde-like material and its clinical application in stroke patients. J Clin Pathol. 1980 Oct;33(10):973–976. doi: 10.1136/jcp.33.10.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sato Y., Hotta N., Sakamoto N., Matsuoka S., Ohishi N., Yagi K. Lipid peroxide level in plasma of diabetic patients. Biochem Med. 1979 Feb;21(1):104–107. doi: 10.1016/0006-2944(79)90061-9. [DOI] [PubMed] [Google Scholar]
  25. Simpson P. J., Lucchesi B. R. Free radicals and myocardial ischemia and reperfusion injury. J Lab Clin Med. 1987 Jul;110(1):13–30. [PubMed] [Google Scholar]
  26. Slater T. F. Free-radical mechanisms in tissue injury. Biochem J. 1984 Aug 15;222(1):1–15. doi: 10.1042/bj2220001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stringer M. D., Görög P. G., Freeman A., Kakkar V. V. Lipid peroxides and atherosclerosis. BMJ. 1989 Feb 4;298(6669):281–284. doi: 10.1136/bmj.298.6669.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Uraizee A., Reimer K. A., Murry C. E., Jennings R. B. Failure of superoxide dismutase to limit size of myocardial infarction after 40 minutes of ischemia and 4 days of reperfusion in dogs. Circulation. 1987 Jun;75(6):1237–1248. doi: 10.1161/01.cir.75.6.1237. [DOI] [PubMed] [Google Scholar]
  29. Weiner J. M., Astein C. S., Arthur J. H., Pirzada F. A., Hood W. B., Jr Persistence of myocardial injury following brief periods of coronary occlusion. Cardiovasc Res. 1976 Nov;10(6):678–686. doi: 10.1093/cvr/10.6.678. [DOI] [PubMed] [Google Scholar]
  30. Werns S. W., Shea M. J., Mitsos S. E., Dysko R. C., Fantone J. C., Schork M. A., Abrams G. D., Pitt B., Lucchesi B. R. Reduction of the size of infarction by allopurinol in the ischemic-reperfused canine heart. Circulation. 1986 Mar;73(3):518–524. doi: 10.1161/01.cir.73.3.518. [DOI] [PubMed] [Google Scholar]

Articles from British Heart Journal are provided here courtesy of BMJ Publishing Group

RESOURCES