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(A) Schematic representation of TMB oxidation by H2O2 and Fe3O4 NPs as peroxidase enzyme and (B) UV–Visible spectra of the synthesized Fe3O4 NPs in PBS buffer (50 mM) pH 7.4 with 0.00208 M TMB (in acetate buffer pH 4.5), and 0.1 M H2O2.