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. 2023 Jun 5;12(11):2223. doi: 10.3390/plants12112223

Orchidaceae in Puglia (Italy): Consistency, Distribution, and Conservation

Alessio Turco 1, Antonella Albano 1, Pietro Medagli 1, Saverio D’Emerico 2, Robert Philipp Wagensommer 3,*
Editor: Jung Sung Kim
PMCID: PMC10255703  PMID: 37299203

Abstract

A study of the distribution of orchid species in Puglia, based on an analysis of 2084 bibliographic reports from 2000 to 2022, was carried out with the aim of revising and updating the information on the consistency of the Orchidaceae family in Puglia, with a special focus on assessing threatened species occurring inside and outside protected areas. The work presents a checklist of the Orchidaceae taxa (genera, species, and subspecies) found in the region, including observations on genera and species that present taxonomic challenges. A total of 113 taxa (i.e., species and subspecies), distributed across 16 genera, are listed in alphabetical order. The most representative genera were Ophrys (51 taxa), Serapias (15 taxa), and Epipactis (11 taxa). Additionally, 49 taxa (43.4%) were found to be endemic to Italy, with 21 of these, mostly belonging to the Ophrys genus, being exclusive to Puglia. Our study notes two different trends of distribution: a predominantly coastal distribution for orchid records located in southern Puglia (the Salento peninsula) and a more widespread distribution for the other provinces. Our study also shows that the greatest number of records locate orchids in protected areas with a positive correlation between their presence and habitats cited in Directive 92/43/EEC.

Keywords: flora, orchids, protected areas, taxonomical concerns

1. Introduction

The Orchidaceae family is a diverse group of plants and one of the most abundant among angiosperms, with over 28,000 accepted species and 763 genera. Many of these species are endemic to small areas and are considered rare and endangered [1,2]. Unfortunately, orchids are at a high risk of extinction, primarily due to the loss of their habitats and the effects of climate change [3]. However, due to their significant conservation value, Orchidaceae is a prominent plant family that receives attention from ecologists, biologists, and environmentalists worldwide [1]. In Europe, orchids are present in almost all habitats, and southern Europe, particularly the Mediterranean, has the highest species diversity. This region is considered the birthplace of certain orchid genera, such as Ophrys and Serapias, which boast remarkable diversity [3].

The Orchidaceae family is well represented in the native vascular flora of Italy, with 236 taxa (species and subspecies) within 27 genera. Among these, the Ophrys genus is particularly species-rich, with 103 taxa. When considering only Italian endemics, the Orchidaceae family is represented by 87 taxa, ranking below the Asteraceae, Plumbaginaceae, and Caryophyllaceae families. The Ophrys genus has 61 endemic taxa, with smaller numbers in the Hieracium, Limonium, and Centaurea genera [4]. The Puglia region has the highest orchid biodiversity, with 101 taxa out of 2552 native plants, followed by Tuscany (100 taxa) and Basilicata (97 taxa) [5]. In terms of Italian endemics, 31 species and subspecies of Orchidaceae occur in Puglia [4], many of which are related to eastern Mediterranean taxa, indicating its relative phytogeographical isolation with respect to other parts of southern peninsular Italy [6].

Puglia is an area of significant interest in terms of flora and vegetation due to its geological history and biogeographical location. The Orchidaceae family has been the focus of numerous botanical studies, including floristic surveys of specific areas and more in-depth investigations of the genetics and morphometry of certain taxa. These studies have often led to the discovery of new specific or subspecific taxa.

The earliest reports on the ancient local flora were conducted by researchers such as Baselice in 1812 for Gargano [7], Marinosci in 1870 [8], Groves in 1887 for Salento [9], and Palanza in 1900 for Bari area [10], all of which are considered pioneering. Later, more modern and systematic specialized studies of Mediterranean Orchidaceae were carried out, mainly by foreign authors. Nelson in 1968 [11]; the spouses Danesch (who described several new species and hybrids) in 1972 [12]; Baumann and Künkele in monographs on Ophrys and Serapias genera, respectively [13,14]; and Lorenz [15] for Serapias all made significant contributions. Additionally, cartographical studies were conducted by Gölz and Reinhard in 1982 for central-southern Puglia [16] and by Lorenz and Gembardt in 1987 for Gargano [17]. More recent research on orchids in Puglia has been published by Romolini and Souche [18], Delforge [19], and GIROS [20]. Souche in 2008 also provided a paper on the study of hybrid species [21].

The aim of this paper is to revise and update the information on the consistency of the Orchidaceae family in Puglia. This involves aligning the terminology with the latest taxonomy adopted by GIROS (Gruppo Italiano per la Ricerca sulle Orchidee Spontanee—Italian Group for Research on Spontaneous Orchids), examining the distribution in the region and recognizing the significance of conserving these species, which are frequently uncommon, endemic to restricted areas, and/or have their locus classicus in Puglia. These plants are employed as bioindicators in environmental management and the safeguarding of habitats.

2. Materials and Methods

2.1. Taxonomic References and Data Analysis

To evaluate the consistency and distribution of Orchidaceae in Puglia, an updated list of reports (Table S1) was created. Due to the high degree of nomenclature variation and the considerable number of new species, and in order to minimize attribution errors, records were sought only among new data published in the period 2000–2022, mainly, but not only, belonging to specialist papers (i.e., mainly from GIROS and GIROS Orch. Spont. Eur.). For each analysed paper, floristic list, occurring site, and geographic coordinates of the study site were noted. If the geographical coordinates were not given, they were subsequently obtained on the basis of other information on the occurring site cited. For all records, the geographic coordinates were converted into WGS 84 system coordinates.

Table S1 contains all the previously mentioned information except for the coordinates of records that were used for cartographic processing, which were not included for conservation purposes and to avoid misuse.

For each taxon, the corotype was also added, taken either from GIROS [20] or from recent publications regarding new species.

Overly generic records (e.g., Serapias without mentioning species attributes) were not considered in the dataset.

The checklist does not include the presence in Puglia of Dactylorhiza maculata (L.) Soó subsp. fuchsii (Druce) Hyl. [22,23,24] as, following Bartolucci et al. [4], it is considered an erroneous report. Himantoglossum adriaticum H.Baumann [25] is excluded for the same reason.

The reports of Ophrys tardans for Bosco Cuturi [26] and Bosco Difesa Grande [27] were considered to be Ophrys expansa (Lumare & Medagli) Biagioli, Kreutz, Lumare, Medagli & De Simoni, as stated by Lumare and Medagli [28].

All the reports for Ophrys holosericea subsp. holosericea (Burm. f.) Greuter were deleted from the checklist, in agreement with GIROS [20]. In addition, reports in which the identifications were recorded as “s.l.” (e.g., O. fusca s.l. and O. lutea s.l.) were deleted as they were considered too generic to be attributed to a species.

The reports of Anacamptis papilionacea subsp. aegaea (P.Delforge) L.Lewis & Kreutz for the Salento peninsula [29,30] were considered to be synonymous with Anacamptis papilionacea (L.) R.M.Bateman, Pridgeon & M.W.Chase due to the limited differences between these specimens and the type species.

The reports of Ophrys passionis Sennen for Puglia [31,32,33,34,35,36] were considered as Ophrys garganica E.Nelson ex O.Danesch et E.Danesch as O. passionis seems to be limited in its distribution to Spain and France [20].

The nomenclature of species follows Delforge [19] and the novelties in Biagioli et al. [37].

On the basis of geolocation data (and subsequent interpretation of satellite images) and other data reported in publications, the genera were correlated, applying our own expertise, with the habitats of Directive 92/43/EEC.

All maps were drawn using QGis ver. 3.22.16 LTR (www.qgis.org, accessed on 20 April 2023). Data analysis used all data from the database, including multiple reports from the same locality, assuming that the more times a species is reported, the greater its abundance in the investigated area.

2.2. Study Area

Puglia is an administrative region in southeastern Italy, covering an area of 19.541 km2. Its territory is mainly made up of plains (53.2%) and hills (45.3%), with mountains accounting for a very limited portion (1.5%) concentrated in the northern part of the region. Its coasts, washed by the Adriatic and Ionian seas, are partly sandy and partly rocky and have a total length of 840 km [38].

Puglia includes six provinces: Bari, BA (today, the metropolitan city of Bari); Foggia, FG; Barletta-Andria-Trani, BT; Taranto, TA; Brindisi, BR; and Lecce, LE. The largest province is Foggia (7008 km2), followed by Bari (3863 km2), Lecce (2799 km2), Taranto (2467 km2), Brindisi (1861 km2), and Barletta-Andria-Trani (1543 km2) [39].

3. Results

3.1. Floristic Data

Floristic data on the presence and distribution of orchids in Puglia were obtained by means of bibliographical research, which involved analysing 185 specialized papers containing 2084 reports (Table S1), with the aim of assessing the consistency and distribution of orchids in Puglia.

As listed in Table 1, orchids in Puglia are represented by 113 taxa (species and subspecies) within 16 genera. The genus with the most taxa is Ophrys (51), followed by Serapias (15) and Epipactis (11), while the genera with the least taxa are Himantoglossum and Platanthera (both 2), and Coeloglossum, Epipogium, Gymnadenia, Limodorum, Neottia, and Spiranthes (all one).

Table 1.

Genera and number of taxa (species and subspecies) per genus of orchids in Puglia derived from analysis of 2084 records.

Genus Number of Taxa Genus Number of Taxa
Anacamptis 8 Limodorum 1
Cephalanthera 3 Neotinea 4
Coeloglossum 1 Neottia 1
Dactylorhiza 3 Ophrys 51
Epipactis 11 Orchis 8
Epipogium 1 Platanthera 2
Gymnadenia 1 Serapias 15
Himantoglossum 2 Spiranthes 1
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On the basis of the geolocation of each report (or, in cases in which the geographical coordinates were not given, the description of the province/municipality or area of discovery), the distribution of genera by province is given in Table 2.

Table 2.

Orchid genera by province, with the percentage of the total orchid genera, derived from analysis of 2084 records.

Province Number of Genera %
BA 12 75
BR 4 25
BT 2 12.5
FG 15 94
LE 9 56
TA 13 81
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As expected, in accordance with the results of Table 1, taking into account the total number of reports, Ophrys is the most frequently reported genus, accounting for 52.93% of records, followed by Serapias (19.04%) and Anacamptis (14.1%), while Coeloglossum, Epipogium, Gymnadenia Spiranthes, and Neottia are cited less frequently. Analytical data are presented in Table 3.

Table 3.

Number of reports of Orchidaceae genera, with the percentage of the total orchid genera, based on the examined 2084 records.

Genus Number of Reports %
Anacamptis 294 14.1
Cephalanthera 15 0.72
Coeloglossum 1 0.05
Dactylorhiza 10 0.5
Epipactis 46 2.2
Epipogium 1 0.05
Gymnadenia 3 0.14
Himantoglossum 39 1.87
Limodorum 15 0.72
Neotinea 52 2.49
Neottia 8 0.38
Ophrys 1103 52.93
Orchis 77 3.69
Platanthera 16 0.77
Serapias 397 19.04
Spiranthes 7 0.33
Total 2084 100
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Considering the single reports obtained by bibliographical research, Table 4 lists the number of records for each genus by province. For the province of Bari, Ophrys, Anacamptis, and Serapias were the most frequently reported genera, as they were in other provinces excluding Barletta-Andria-Trani and Foggia. The latter, with the highest number of genera in the region, showed a similar trend but with the addition of the genera Epipactis and Orchis. In contrast, the province of Barletta-Andria-Trani had the lowest number of reports.

Table 4.

Reports for each genus by province, with the percentage of the provincial total, based on the examined 2084 records.

Genus BA BR BT FG LE TA
N % N % N % N % N % N %
Anacamptis 74 13.2 20 29.0 50 9.2 110 15.5 40 20.3
Cephalanthera 1 0.2 13 2.4 1 0.5
Coeloglossum 1 0.2
Dactylorhiza 3 0.5 5 0.9 2 1.0
Epipactis 2 0.4 39 7.2 3 0.4 2 1.0
Epipogium 1 0.2
Gymnadenia 3 0.6
Himantoglossum 21 3.7 11 2.0 2 0.3 5 2.5
Limodorum 4 0.7 6 1.1 2 0.3 3 1.5
Neotinea 22 3.9 1 1.4 16 2.9 7 1.0 6 3.0
Neottia 7 1.3 1 0.5
Ophrys 332 59.1 28 40.6 1 50.0 300 55.2 331 46.6 111 56.3
Orchis 27 4.8 1 50.0 44 8.1 1 0.1 4 2.0
Platanthera 7 1.2 6 1.1 3 1.5
Serapias 65 11.6 20 29.0 41 7.6 253 35.6 18 9.1
Spiranthes 4 0.7 2 0.3 1 0.5
Total 562 100 69 100 2 100 543 100 711 100 197 100
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Considering the corotypes of the analysed orchids, there are 49 taxa (species and subspecies) endemic to Italy, with 620 records, and 3 subendemic taxa (i.e., with a restricted distribution that includes Italy and a small area beyond its border), with 23 records. With 36 taxa, Ophrys is the richest genus in terms of endemism, followed by Serapias (8 taxa), Epipactis (4 taxa), and Anacamptis (1 taxon). Ophrys also accounts for the only three subendemics.

Table 5 shows the number of records for each genus by province for taxa (species and subspecies) endemic to Italy. In this case, the province of Lecce has the highest number of endemic taxa, followed by the provinces of Foggia and Bari. As previously mentioned, all endemics belong to the genera Ophrys and Serapias, except in the province of Foggia, which also has endemics belonging to Anacamptis and Epipactis.

Table 5.

Distribution of records of taxa (species and subspecies) endemic to Italy, by genus and province, based on 620 reports.

Genus BA BR BT FG LE TA
N % N % N % N % N % N %
Anacamptis 1
Epipactis 18
Ophrys 171 7 1 159 148 56
Serapias 4 5 5 44 1
Total 175 100 12 100 1 100 183 100 192 100 57 100
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Table 6 shows the correlation between genera and habitats cited in Directive 92/43 EEC. Taxa within the genus Ophrys are seen to occupy the highest number of habitats (10), ranging from humid environments (i.e., habitat 1410) to xeric Mediterranean grasslands and forests. This is obviously also related to the high species diversity of this genus. In contrast, some genera are associated with a specific habitat group, as in the case of Himantoglossum and Spiranthes, which grow in xeric Mediterranean grasslands, and Epipactis, Limodorum, Platanthera, Neottia, Cephalanthera, Coeloglossum, and Epipogium, which prefer woody habitats.

Table 6.

Presence of orchids in habitats cited in Directive 92/43/EEC (1410 Mediterranean salt meadows; 2270* wooded dunes with pines; 5420 Sarcopoterium spinosum phryganas; 6210*, 6220*, and 62A0 xeric Mediterranean grasslands; 91H0* and 91M0 Pannonian woods; 9210* Apennine beech forests; 9250 Quercus trojana woods; 9260 Castanea sativa woods; 9340 Quercus ilex and Quercus rotundifolia forests; 9540 Mediterranean pine forests with endemic Mesogean pines).

Natura 2000 Code 1410 2270* 5420 6210* 6220* 62A0 91H0* 91M0 9210* 9250 9260 9340 9540
Anacamptis • • • • • •
Cephalanthera •
Coeloglossum •
Dactylorhiza • • •
Epipactis • • •
Epipogium •
Gymnadenia •
Himantoglossum • • •
Limodorum • • •
Neotinea • • • • •
Neottia • •
Ophrys • • • • • • • • • •
Orchis • • • •
Platanthera • • •
Serapias • • • • •
Spiranthes • •
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3.2. Checklist of Orchids in Puglia

The updated checklist of Orchidaceae in Puglia includes 113 taxa (species and subspecies). Genera and species are alphabetically listed. “E” before the name of the species means endemic to Italy, while “SubE” means subendemic, i.e., with a restricted distribution that includes Italy and a small area beyond its border.

Genus Anacamptis Rich.
E Anacamptis berica Doro [40]
Anacamptis collina (Banks & Sol. ex Russell) R.M.Bateman, Pridgeon & M.W.Chase [22,27,31,32,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55]
Anacamptis fragrans (Pollini) R.M.Bateman [22,27,44,53,54,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74]
Anacamptis laxiflora (Lam.) R.M.Bateman, Pridgeon & M.W.Chase [27,59,64,65,75,76,77,78,79,80,81,82,83]
Anacamptis morio (L.) R.M.Bateman, Pridgeon & M.W.Chase [22,27,29,30,31,32,33,34,42,43,44,45,46,47,48,49,50,52,53,54,56,58,60,61,62,63,64,65,67,68,70,73,74,75,78,80,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102]
Anacamptis palustris (Jacq.) R.M.Bateman, Pridgeon & M.W.Chase [56,64,65,75,77,79,80,103,104,105,106,107]
Anacamptis papilionacea (L.) R.M.Bateman, Pridgeon & M.W.Chase [22,27,28,29,30,32,33,34,35,42,43,44,45,46,47,48,49,50,52,53,54,56,57,60,61,62,63,65,67,68,70,73,80,86,88,89,90,91,93,98,99,100,101,108,109,110,111,112,113]
Anacamptis pyramidalis (L.) Rich. [22,27,28,35,42,44,47,49,52,53,56,58,60,61,62,63,64,66,68,70,71,72,73,78,80,84,86,88,89,90,92,93,94,95,98,101,103,108,110,113,114,115,116,117,118,119]
Genus Cephalanthera Rich.
Cephalanthera damasonium (Mill.) Druce [23,24,27,84,117,120]
Cephalanthera longifolia (L.) Fritsch [24,120]
Cephalanthera rubra (L.) Rich. [24,121]
Genus Coeloglossum Hartm.
Coeloglossum viride (L.) Hartm. [22]
Genus Dactylorhiza Neck. ex Nevski
Dactylorhiza romana (Sebast.) Soó [22,27,46,61,111,122]
Dactylorhiza saccifera (Brongn.) Soó [48,121,122]
Dactylorhiza sambucina (L.) Soó [22]
Genus Epipactis Zinn
Epipactis exilis P.Delforge [123]
E Epipactis garganica S.Hertel [124]
Epipactis helleborine (L.) Crantz [24,27,117,121,125,126,127,128]
E Epipactis meridionalis H.Baumann & R.Lorenz [121,127]
Epipactis microphylla (Ehrh.) Sw. [24,27,84,115,117,121,125,129]
Epipactis muelleri Godfery [24,121]
Epipactis neglecta (Kümpel) Kümpel [125]
Epipactis palustris (L.) Crantz [103,130,131]
Epipactis purpurata Sm. [123,127]
E Epipactis schubertiorum Bartolo, Pulv., & Robatsch [24,121]
E Epipactis umbrae (Kreutz, A.Rossini, Quitadamo, Turco & Medagli) Biagioli, Kreutz, A.Rossini, Quitadamo, Turco, Medagli & De Simoni [127]
Genus Epipogium Borkh.
Epipogium aphyllum Sw. [121]
Genus Gymnadenia R.Br.
Gymnadenia conopsea (L.) R.Br. [22,31,132]
Genus Himantoglossum Spreng.
Himantoglossum hircinum (L.) Spreng. [22,27,42,46,90,102,111,117]
Himantoglossum robertianum (Loisel.) P.Delforge [22,27,31,32,33,41,42,44,46,48,49,50,51,52,54,60,61,70,88,90,93,94,111,133,134]
Genus Limodorum Boehm.
Limodorum abortivum (L.) Sw. [24,27,35,48,50,52,56,61,65,69,70,84,103,117,132]
Genus Neotinea Rchb.f.
Neotinea lactea (Poir.) R.M.Bateman, Pridgeon & M.W.Chase [27,28,42,44,52,53,56,58,68,78,92]
Neotinea maculata (Desf.) Stearn [93]
Neotinea tridentata (Scop.) R.M.Bateman, Pridgeon & M.W.Chase [22,27,31,42,48,49,50,58,61,67,68,70,74,86,101,116,119]
Neotinea ustulata (L.) R.M.Bateman, Pridgeon & M.W.Chase [22,27,42,49,67,74,91,101,113,114,116,119,135]
Genus Neottia Guett.
Neottia nidus-avis (L.) Rich. [22,23,24,48,117,121,136]
Genus Ophrys L.
Ophrys apifera Huds. [22,27,35,46,48,61,65,69,70,75,76,79,80,81,82,83,101,103,104,108,111,115,116,119,120,132,137,138,139]
E Ophrys apulica (O.Danesch & E.Danesch) O.Danesch & E.Danesch [27,28,31,32,33,35,36,41,42,44,46,47,49,50,52,53,56,57,60,61,62,63,64,65,67,68,69,70,71,72,73,75,76,80,81,82,83,89,90,91,92,93,100,101,107,109,111,112,113,115,117,119,139,140,141,142,143,144,145,146,147,148,149,150]
SubE Ophrys archipelagi Gölz & H.R.Reinhard [41,68]
Ophrys bertolonii Moretti [22,27,28,29,32,33,35,36,42,43,44,46,47,49,50,52,53,54,56,57,60,61,62,63,64,65,67,68,69,70,72,73,79,80,86,87,88,89,90,91,93,95,98,100,101,107,110,113,114,116,119,120,138,139,147,148,151,152,153]
E Ophrys bertoloniiformis O.Danesch & E.Danesch [22,31,54,68,70,99,140,154,155]
E Ophrys biscutella O.Danesch & E.Danesch [31,48,49,60,119,154,156,157]
Ophrys bombyliflora Link [27,28,29,32,33,34,35,42,43,44,46,48,49,50,52,53,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,73,75,76,78,79,80,81,82,83,87,88,89,90,91,98,100,101,109,110,111,112,114,119,138,139,142,143,147,150,152,154,158,159,160]
E Ophrys brutia P.Delforge [36,50,68,91,142,148,158,161,162]
SubE Ophrys calocaerina Devillers-Tersch. & Devillers [163]
Ophrys candica (E.Nelson ex Soó) H.Baumann & Künkele [44,47,52,53,56,62,63,65,72,92,98,107,115,143,147,159,164,165,166]
E Ophrys celiensis O.Danesch & E.Danesch [27,35,50,61,70,93,100,111,113,139,155,167]
E Ophrys cinnabarina Romolini & Soca [27,31,35,46,69,101,111,116,139,140,144]
E Ophrys classica Devillers-Tersch. & Devillers [31,42,48,53,56,68,70,74,86,90,103,107,112,114,117,140,142,144,145,148,154,158,160,162]
E Ophrys conradiae Melki & Deschatres [27,35,46,48,67,89,101,111,139,168,169,170]
SubE Ophrys corsica Soleirol ex G.Foelsche & W.Foelsche [27,33,36,48,49,53,70,91,98,101,110,147,154,171]
Ophrys eleonorae Devillers-Tersch. & Devillers [31,50,54,131,161,172]
E Ophrys exaltata Ten. [27]
E Ophrys expansa (Lumare & Medagli) Biagioli, Kreutz, Lumare, Medagli & De Simoni [26,28,52,53,112,152]
Ophrys funerea Viv. [22,27,60,137,172,173]
E Ophrys garganica E.Nelson ex O.Danesch & E.Danesch [22,27,28,29,31,32,33,34,35,36,41,42,44,46,48,50,53,54,56,57,59,64,65,66,67,68,70,71,75,76,78,80,81,87,88,89,90,91,93,94,99,100,101,109,111,112,113,114,117,119,131,138,139,142,143,144,145,148,150,152,155,158,160,161,163]
E Ophrys gracilis (Büel, O.Danesch & E.Danesch) Englmaier [27,31,35,48,89,101,119,132,140,157]
E Ophrys gravinensis D’Alonzo [5,49,67,91,114]
Ophrys incubacea Bianca [27,28,32,33,34,35,42,44,46,48,49,50,52,53,57,58,60,61,62,63,64,66,67,68,69,70,71,75,76,78,80,81,87,88,89,90,91,93,95,97,100,101,107,109,110,111,112,113,114,117,120,133,138,139,142,143,145,148,149,150,152,158]
E Ophrys ingrassiae (Dura, Turco, Gennaio & Medagli) Biagioli, Kreutz, Dura, Turco, Gennaio, Medagli, & De Simoni [5,61,72,93]
E Oprhys japigiae Turco, D’Emerico, Dura, Gennaio & Medagli [172]
Ophrys lacaitae Lojac. [48,69,89,101,116,119,132,138,140,155,157,174]
E Ophrys lojaconoi P.Delforge [27,31,48,50,54,140,144,161,172,173,175]
E Ophrys lucana P.Delforge, Devillers-Tersch. & Devillers [27,61,70,116,120,133,172,176]
E Ophrys lucifera Devillers-Tersch. & Devillers [27,41,109,118,158,173]
E Ophrys lupercalis Devillers-Tersch. & Devillers [65,109,118,141,161,172,175]
Ophrys lutea Cav. [27,28,29,32,33,34,35,42,43,44,48,50,53,57,58,60,61,63,64,65,68,69,70,71,73,80,84,85,86,88,91,94,95,97,98,110,112,119,133,138,139,142,147,152,154,158]
E Ophrys mateolana Medagli, D’Emerico, Bianco & Ruggiero [27,42,46,49,68,88]
E Ophrys mattinatae Medagli, A.Rossini, Quitadamo, D’Emerico & Turco [5,177]
E Ophrys minipassionis Romolini & Soca [27,31,48,70,140,154]
E Ophrys montis-gargani (Van de Vijver & W.Looken) Biagioli, Kreutz & De Simoni [17,144,178]
E Ophrys murgiana Cillo, Medagli & Margh. [5,33,87]
Ophrys neglecta Parl. [22,27,28,29,31,32,34,35,42,43,44,46,48,49,50,52,54,56,57,58,60,61,62,63,64,65,67,68,69,70,71,72,73,80,88,89,91,92,93,94,95,98,99,100,101,102,103,109,110,111,112,113,114,118,119,120,138,139,140,143,147,148,152,154,158,163,165,179]
E Ophrys panormitana (Tod.) Soó [180]
E Ophrys paolina (V.Liverani & Romolini) Biagioli, Kreutz, V.Liverani, Romolini & De Simoni [48,69,89,101]
E Ophrys parvimaculata (O.Danesch & E.Danesch) Paulus & Gack [27,36,42,46,48,49,52,67,68,69,70,88,89,91,93,98,109,113,115,141,142,147,148,149,157,158,159,161,163,181]
E Ophrys peucetiae Lozito, D’Emerico, Medagli & Turco [5,109,141]
E Ophrys pinguis Romolini & Soca [182]
E Ophrys pollinensis E.Nelson ex Devillers-Tersch. & Devillers [93]
E Ophrys promontorii O.Danesch & E.Danesch [22,27,89,155]
E Ophrys pseudomelena Turco, Medagli & D’Emerico [5,53,109,141]
Ophrys sicula Tineo [22,27,32,33,36,41,42,44,47,48,49,50,53,54,56,57,59,60,61,63,64,67,68,70,71,73,78,89,90,91,93,94,98,99,100,109,110,111,112,114,118,119,138,147,152,154]
E Ophrys sipontensis (Gumpr.) O.Danesch & E.Danesch [27,31,33,36,48,49,50,54,91,144,149]
Ophrys speculum Link [22,33,47,113,145,155,183]
E Ophrys tardans O.Danesch & E.Danesch [5,53,57,95,110,138,146,151,153,164,166,179,184,185]
E Ophrys tarentina Gölz & H.R.Reinhard [27,47,52,53,68,70,84,94,108,112,148,150,163]
E Ophrys tarquinia P.Delforge [186]
Genus Orchis L.
Orchis anthropophora (L.) All. [22,27,32,33,42,43,48,49,60,61,69,88,89,91,117,120]
Orchis italica Poir. [22,27,32,42,48,50,52,58,60,61,66,69,70,88,89,90,94,99,101,113,116,117,119,120]
Orchis mascula (L.) L. [120]
Orchis pauciflora Ten. [22,23,74]
Orchis provincialis Balb. ex Lam. & DC. [23,69]
Orchis purpurea Huds. [23,27,113,117,120]
Orchis quadripunctata Cirillo ex Ten. [22,48,101,116]
Orchis simia Lam. [27,46,111,116,187]
Genus Platanthera Rich.
Platanthera bifolia (L.) Rich. [188]
Platanthera chlorantha (Custer) Rchb. [22,27,35,46,48,50,61,69,70,84,111,121,132,189]
Genus Serapias L.
E Serapias apulica (H.Baumann & Künkele) P.Delforge [5,27,54,57,58,64,78,81,92,95,190,191,192,193,194,195,196,197,198,199,200,201,202]
E Serapias ausoniae Gennaio & Pellegrino [203]
Serapias bergonii E.G.Camus [27,34,52,53,57,60,61,63,64,68,70,73,81,82,92,95,97,142,158,159,190,192,193,194,196,197,198,199,200,201]
E Serapias brundisina (Lumare & Medagli) Biagioli, Kreutz, Lumare, Medagli & De Simoni [95,97,202]
Serapias cordigera L. [27,34,35,46,48,50,53,64,69,70,72,73,78,79,92,101,111,116,119,142,190,192,193,194,196,198,199,200,201,204]
E Serapias guadinae Lumare, Medagli & Biagioli [201]
Serapias lingua L. [22,27,31,32,35,46,48,49,50,53,56,60,61,62,63,64,65,68,69,70,72,73,78,80,83,88,89,91,92,95,96,97,100,111,114,158,192,193,194,196,197,199,200]
E Serapias messapica (Lumare & Medagli) Biagioli, Kreutz, Lumare, Medagli & De Simoni [95,202]
E Serapias neretina (Lumare & Medagli) Biagioli, Kreutz, Lumare, Medagli & De Simoni [52,95,202]
Serapias parviflora Parl. [22,27,32,33,35,46,47,49,52,53,56,57,60,61,63,64,65,68,69,70,72,73,75,79,80,81,82,83,90,91,92,95,96,97,98,109,111,113,133,159,192,193,194,196,198,199,200,203,205]
Serapias politisii Renz [52,53,57,62,63,64,72,73,75,76,92,95,96,98,142,158,159,190,192,193,194,196,199,200,201,204,205]
E Serapias sallentina (Lumare & Medagli) Biagioli, Kreutz, Lumare, Medagli & De Simoni [92,194,198,204]
E Serapias uxentina Gennaio [81,82,83]
Serapias vomeracea (Burm.f.) Briq. subsp. vomeracea [22,27,32,33,35,42,43,46,48,49,50,53,61,63,64,65,67,69,70,71,73,74,76,79,81,83,85,89,90,91,92,95,96,100,101,102,103,111,113,114,115,116,118,119,120,142,158,190,192,193,194,196,198,199,200,201,204,206,207]
Serapias vomeracea (Burm.f.) Briq. subsp. longipetala (Ten.) Baumann & Künkele [53,57,62,63,64,81,82,92,95,96,97,142,192,193,194,196,198,199,200,201,204,208]
Genus Spiranthes Rich.
Spiranthes spiralis (L.) Chevall. [27,42,44,53,61,70]
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4. Discussion

Our study presents the findings of bibliographical research on orchids in Puglia covering the years from 2000 to 2022. We opted to focus on data from the last two decades, mainly from specialized journals, because they provided more precise determinations, particularly for taxonomically critical genera such as Ophrys, Serapias, and Epipactis. Additionally, there have been ongoing nomenclatural changes, and other taxa have been added in recent years. In this study, we considered not only the presence of orchids but also their location, distribution, and preferred habitats.

Thanks to this research project, the orchidological biodiversity of Puglia has been updated to include 113 taxa (species and subspecies), of which 49 (43.4%) are endemic to Italy. These numbers exceed those reported in previous studies. For instance, the authors of [4] reported only 31 endemic taxa, and those of [209] reported 42 taxa, while our research found 52 endemic/subendemic taxa. The same trend was observed in the overall number of species. Among the endemics, 21 taxa (species and subspecies), mostly belonging to the genus Ophrys, are exclusive to Puglia. This growth is also consistent with what was recently asserted in [210].

The distribution of records was used to draw up a map of orchids across Puglia using geolocation data extracted from publications and other information that helped us to geolocate the study areas (Figure 1, Table S1). As can be seen, most of the records for the Salento peninsula are situated near the coastline, indicating that a significant portion of the biodiversity of this area in terms of environments and habitats is concentrated there. Indeed, the Salento peninsula is characterized mainly by urban areas and agroecosystems, which have eroded almost all the biodiversity located inland. In contrast, the records are more dispersed in the rest of Puglia, where woody environments are more extensive and abundant in addition to coastal and transitional environments.

Figure 1.

Figure 1

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Orchid genera across Puglia based on the examined 2084 records.

It is worth noting that a significant majority of orchid records are found within protected areas such as parks, SCIs, and SACs (Figure 2). This reinforces the significance of these areas not only for conservation purposes but also as areas that can be used for research activities by naturalists and scientists.

Figure 2.

Figure 2

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Distribution of orchid genera (based on the examined 2084 records) and protected areas across Puglia.

Based on our data, the province with the highest number of records is Lecce, followed by Bari, Foggia, Taranto, Brindisi, and Barletta-Andria-Trani (Table 4). The limited availability of data for the BT province can not only be attributed to its recent establishment in 2009 but also to its small size and because it has clearly been poorly investigated by botanists and naturalists.

Referring to Table 2 and considering Lorenz and Gembardt’s [17] published report on Spiranthes spiralis in the province of Foggia, the distribution of genera by province is presented in Figure 3.

Figure 3.

Figure 3

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Percentage (%) of orchid genera in Puglia present in each province.

The trend observed in the distribution of species and reports by province seems to differ from the distribution of genera depicted in Figure 3. Despite having a lower number of genera, the provinces of Bari and Lecce exhibit a higher richness of taxa (species and subspecies) than the province of Taranto. Meanwhile, Foggia maintains its status as the province with the highest species richness (Figure 4).

Figure 4.

Figure 4

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Percentage (%) of orchid taxa (species and subspecies) in Puglia by province.

In terms of species reports, the province of Bari has the highest number of reports for the genera Ophrys and Himantoglossum, while the province of Lecce has the majority of reports for the genera Serapias and Anacamptis, and the province of Foggia has the highest number of reports for the genera Orchis and Epipactis.

Puglia serves as the southernmost boundary for certain Italian species such as Anacamptis palustris, Ophrys funerea, O. lucifera, O. parvimaculata, and O. minipassionis. However, it also marks the northernmost boundary for other taxa such as Epipactis aspromontana, E. schubertiorum, Ophrys celiensis, O. sipontensis, and O. tarentina, as stated by Pezzetta [209].

5. Conclusions

In conclusion, our study provides an updated and comprehensive checklist of orchid species and subspecies found in Puglia, including taxonomic observations and assessments of threatened species within and outside of protected areas. Our study, based on a thorough analysis of bibliographic reports spanning over two decades, contributes significantly to the understanding of Orchidaceae diversity in the region.

The checklist of 113 taxa (species and subspecies) across 16 genera also provides a comprehensive overview, highlighting taxonomic challenges and the prevalence of certain genera such as Ophrys, Serapias, and Epipactis compared to other genera. The presence of 49 taxa endemic to Italy, with a substantial number exclusive to Puglia, underscores the region’s significance for orchid biodiversity and conservation. In this context, it must be highlighted that all new species novelties were added in the checklist, including that of Ophrys panormitana [180], for which further investigations should be made to exclude the hybridogenic origin of the plants, as only one plant was found with several tufts of vegetative origin, which would suggest a morphological convergence rather than the presence of a new species, in this case, endemic to Sicily.

The distribution patterns reveal distinct trends, with a strong coastal preference in the south of Puglia and a more widespread distribution in other provinces. Importantly, this study underscores the importance of protected areas as critical habitats for orchid populations. Overall, this research enhances our knowledge of Puglia’s orchid flora and emphasizes the need for conservation efforts to safeguard these valuable plant species.

Supplementary Materials

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/plants12112223/s1. Table S1: Examined reports of Orchidaceae in Puglia (Italy).

Click here for additional data file. (126.1KB, zip)

Author Contributions

Conceptualization, A.T., A.A., P.M., S.D. and R.P.W.; methodology, A.T., A.A. and R.P.W.; formal analysis, A.T., A.A. and R.P.W.; investigation, A.T., A.A. and P.M.; resources, A.T. and P.M.; data curation, A.T. and A.A.; writing—original draft preparation, A.T. and A.A.; writing—review and editing, A.T., A.A., S.D. and R.P.W.; visualization, S.D.; supervision, R.P.W.; funding acquisition, R.P.W. All authors have read and agreed to the published version of the manuscript.

Data Availability Statement

Data are contained in the article, including the supplementary material.

Conflicts of Interest

The authors declare no conflict of interest.

Funding Statement

This research received no external funding.

Footnotes

Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

References

  • 1.Khapugin A.A. A global systematic review on orchid data in protected areas. Nat. Conser. Res. 2020;5((Suppl. S1)):19–33. doi: 10.24189/ncr.2020.019. [DOI] [Google Scholar]
  • 2.Bazzicalupo M., Calevo J., Smeriglio A., Cornara L. Traditional, Therapeutic Uses and Phytochemistry of Terrestrial European Orchids and Implications for Conservation. Plants. 2023;12:257. doi: 10.3390/plants12020257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Štípková Z., Kindlmann P. Factors determining the distribution of Orchids—A review with examples from the Czech Republic. Eur. J. Environ. Sci. 2021;11:21–30. doi: 10.14712/23361964.2021.3. [DOI] [Google Scholar]
  • 4.Bartolucci F., Peruzzi L., Galasso G., Albano A., Alessandrini A., Ardenghi N.M.G., Astuti G., Bacchetta G., Ballelli S., Banfi E., et al. An updated checklist of the vascular flora native to Italy. Plant Biosyst. 2018;152:179–303. doi: 10.1080/11263504.2017.1419996. [DOI] [Google Scholar]
  • 5.Wagensommer R.P., Medagli P., Turco A., Perrino E.V. IUCN Red List evaluation of the Orchidaceae endemic to Apulia (Italy) and considerations on the application of the IUCN protocol to rare species. Nat. Conserv. Res. 2020;5((Suppl. S1)):90–101. doi: 10.24189/ncr.2020.033. [DOI] [Google Scholar]
  • 6.Peruzzi L., Conti F., Bartolucci F. An inventory of vascular plants endemic to Italy. Phytotaxa. 2014;168:1–75. doi: 10.11646/phytotaxa.168.1.1. [DOI] [Google Scholar]
  • 7.Baselice G., Rapporto fatto dal Signor D. Michele Tenore Direttore del Real Giardino delle Piante, da Gaetano Baselice corrispondente al detto Real Giardino ec., sulla peregrinazione botanica, eseguita da lui in una parte del Monte Gargano. Viaggio botanico eseguito ne’ circondarj di Manfredonia, Monte S. Angiolo, e S. Marco in Lamis. Resto della Flora de’ suddetti circondarj con qualche osservazione fatta su di essa. Saggio sugl’insetti rinvenuti in questa parte del Gargano. Giorn. Enc. di Napoli. 1812;6:16–70. [Google Scholar]
  • 8.Marinosci M. La Flora Salentina. Editrice Salentina; Lecce, Italy: 1870. 2 voll. [Google Scholar]
  • 9.Groves E. Flora della costa meridionale della Terra d’Otranto. Nuovo Giorn. Bot. Ital. 1887;19:119–219. [Google Scholar]
  • 10.Palanza A. Flora della Terra di Bari. In: Jatta A., editor. La Terra di Bari Sotto L’aspetto Storico, Economico e Naturale. Tipografia dell’editore V. Vecchi; Trani, Italy: 1900. pp. 153–244. [Google Scholar]
  • 11.Nelson E. Serapias, Aceras, Loroglossum, Barlia. Chernez-Montreux; Montreux, Switzerland: 1968. Monographie und Ikonographie der Orchidaceen-Gattungen. [Google Scholar]
  • 12.Danesch O., Danesch E. Orchideen Europas, Ophrys-Hybriden. Hallwag, Bern und Stuttgart; Switzerland, Germany: 1972. [Google Scholar]
  • 13.Baumann H., Künkele S. Die Gattung Ophrys L. Eine taxonomische Übersicht. Mitt. Bl. Arbeitskr. Heim. Orch. Baden-Württ. 1986;18:306–688. [Google Scholar]
  • 14.Baumann H., Künkele S. Die Gattung Serapias L. Eine taxonomische Übersicht. Mitt. Bl. Arbeitskr. Heim. Orch. Baden-Württ. 1989;21:355–700. [Google Scholar]
  • 15.Lorenz R. Die Gattung Serapias in Italien: Arten und Verbreitung. J. Eur. Orch. 2001;33:235–368. [Google Scholar]
  • 16.Gölz P., Reinhard H.R. Orchideen in Süditalien. Mitt. Bl. Arbeitskr. Heim. Orch. Baden-Württ. 1989;14:1–124. [Google Scholar]
  • 17.Lorenz R., Gembardt C. Die Orchideenflora des Gargano (Italien). Ein Beitrag zum OPTIMA—Project “Kartierung der mediterranen Orchideen”. Mitt. Bl. Arbeitskr. Heim. Orch. Baden-Württ. 1987;19:385–756. [Google Scholar]
  • 18.Romolini R., Souche R. Ophrys D’Italia. Sococor; Saint Martin de Londres, France: 2012. [Google Scholar]
  • 19.Delforge P. Guide des Orchidées D’Europe, D’Afrique du Nord et du Proche-Orient. Delachaux et Niestlé; Lonay, France: 2016. [Google Scholar]
  • 20.GIROS . Orchidee D’Italia. 2nd ed. Il Castello; Milano, Italy: 2016. [Google Scholar]
  • 21.Souche R. Hybrides D’Ophrys Duasinn Méditerranèen Occidental. Sococor; Saint Martin de Londres, France: 2008. [Google Scholar]
  • 22.Rossini A., Quitadamo G. Segnalazioni di orchidee rare nel Gargano. GIROS Not. 2002;21:25–27. [Google Scholar]
  • 23.Biondi E., Casavecchia S., Biscotti N. Forest biodiversity of the Gargano Peninsula and a critical revision of the syntaxonomy of the mesophilus woods of southern Italy. Fitosociologia. 2008;45:93–127. [Google Scholar]
  • 24.Rossini A., Quitadamo Q. Epipactis schubertiorum Bartolo, Pulv. & Robatsch prima segnalazione nel Gargano e in Puglia. GIROS Not. 2003;22:25–26. [Google Scholar]
  • 25.Bianco P.M. Le orchidee come indicatori di qualità degli habitat. Biol. Ital. Giugno. 2012;2012:35–48. [Google Scholar]
  • 26.Dura T., Mascia G., Pisconti A., Biagioli M. Prima segnalazione di Ophrys tardans nella provincia di Taranto. GIROS Orch. Spont. Eur. 2017;60:447–451. [Google Scholar]
  • 27.D’Alonzo F. Le orchidee della Murgia barese e materana. Parco Nazionale dell’Alta Murgia barese—Parco Regionale della Murgia materana—Bosco Difesa Grande. GIROS Not. 2013;54:24–38. [Google Scholar]
  • 28.Lumare F., Medagli P. Una nuova entità salentina, Ophrys tenthredinifera subsp. neglecta var. expansa, a confronto con Ophrys tardans. GIROS Orch. Spont. Eur. 2018;61:28–40. [Google Scholar]
  • 29.Lumare F., Medagli P., Biagioli M. Primo ritrovamento in Italia (Salento, Puglia) di Anacamptis papilionacea subsp. aegaea. GIROS Orch. Spont. Eur. 2017;60:83–89. [Google Scholar]
  • 30.Lumare F., Medagli P. Studio morfometrico su Anacamptis papilionacea subsp. aegaea in Salento (Puglia) e suo confronto con A. × gennarii (A. morio × A. papilionacea subsp. papilionacea) GIROS Orch. Spont. Eur. 2018;61:103–113. [Google Scholar]
  • 31.Perilli M. Ritrovamenti nel Gargano interno di Orchidaceae a fiori apo e ipocromatici e di un’intera popolazione di Serapias lingua con lusus fiorali. GIROS Orch. Spont. Eur. 2015;58:338–342. [Google Scholar]
  • 32.D’Alonzo F., Perilli M., Biagioli M. Un nuovo ibrido di Ophrys (O. apulica × O. sipontensis) nella Murgia barese (Puglia centrale) GIROS Orch. Spont. Eur. 2022;65:231–237. [Google Scholar]
  • 33.D’Alonzo F., Biagioli M. Segnalazioni di due entità rarissime nella Puglia centrale (Anacamptis × murginaa e Ophrys speculum) GIROS Orch. Spont. Eur. 2022;65:238–246. [Google Scholar]
  • 34.Gennaio R. Prima segnalazione italiana di xSerapicamptis myrtoa (Anacamptis papilionacea × Serapias bergonii) nel Salento (Puglia meridionale) GIROS Orch. Spont. Eur. 2022;65:247–251. [Google Scholar]
  • 35.D’Alonzo F. Descrizione di un nuovo ibrido di Ophrys in Puglia: Ophrys × difesagrandeana D’Alonzo [O. apulica (O. Danesch & E. Danesch) O. Danesch & E. Danesch × O. conradiae Melki & Deschâtres] GIROS Not. 2014;57:32–34. [Google Scholar]
  • 36.Romolini R., Soca R. Descrizione di dieci nuovi ibridi di Ophrys italiane. GIROS Not. 2014;55:48–68. [Google Scholar]
  • 37.Biagioli M., Kreutz K., De Simoni M.G. Novità tassonomiche per alcuni taxa di Orchidaceae italiane. GIROS Orch. Spont. Eur. 2022;65:149–152. [Google Scholar]
  • 38.Biondi E., Casavecchia S., Beccarisi L., Marchiori S., Medagli P., Zuccarello V. Le serie di vegetazione della Regione Puglia. In: Blasi C., editor. La Vegetazione D’Italia. Palombi Editori; Rome, Italy: 2010. pp. 391–410. [Google Scholar]
  • 39.ISTAT Superfici Delle Unità Amministrative a Fini Statistici. [(accessed on 5 April 2023)]. Available online: https://www.istat.it/it/archivio/82599.
  • 40.Doro D. Anacamptis berica, nuova specie allotetraploide. Giros Orch. Spont. Eur. 2021;64:265–277. [Google Scholar]
  • 41.Rossini A., Quitadamo G. Ophrys lucifera Devilers-Tersch. & Devillers. GIROS Not. 2002;20:12–13. [Google Scholar]
  • 42.Perrino P., Laghetti G., Tersi M. Modern concepts for the sustainable use of Plant Genetic Resources in the Mediterranean natural protected areas: The case study of the Alta Murgia Park (Italy) Genet. Resour. Crop. Evol. 2006;53:695–710. doi: 10.1007/s10722-004-3942-x. [DOI] [Google Scholar]
  • 43.Perrino E.V., Cavallaro V. Flora delle praterie di Monte Castiglione (Alta Murgia-Puglia) Interdipendenze. 2011;3:31–45. doi: 10.4447/int.3110011. [DOI] [Google Scholar]
  • 44.Inguscio S., Rossi E., Scarpina L., Medagli P. Guida Alle Orchidee del Parco Naturale Regionale Porto Selvaggio e Palude del Capitano. Editrice Salentina; Galatina, Italy: 2012. pp. 1–77. [Google Scholar]
  • 45.Lumare F., Medagli P., Biagioli M. Studio nomenclaturale e morfometrico su un raro ibrido del Salento (Puglia): Anacamptis × dafnii nothosubsp. camparonensis (A. collina × A. papilionacea subsp. papilionacea) GIROS Orch. Spont. Eur. 2016;59:427–440. [Google Scholar]
  • 46.D’Alonzo F., Perilli M., Biagioli M. Tre nuovi ibridi naturali di Ophrys nella Murgia materana (Basilicata) e barese (Puglia) GIROS Orch. Spont. Eur. 2017;60:404–420. [Google Scholar]
  • 47.Dura T., Baldacconi R. Seconda segnalazione di Ophrys speculum nella provincia di Taranto (Puglia) GIROS Orch. Spont. Eur. 2018;61:435–438. [Google Scholar]
  • 48.D’Alonzo F., Perilli M., Biagioli M. Quattro nuovi ibridi naturali di Ophrys nel Parco Nazionale del Gargano (Foggia, Puglia settentrionale) GIROS Orch. Spont. Eur. 2018;61:60–78. [Google Scholar]
  • 49.D’Alonzo F., Perilli M., Biagioli M. Sei nuovi ibridi di Ophrys, cinque nella Murgia barese (Puglia) e uno in Basilicata. GIROS Orch. Spont. Eur. 2019;62:320–349. [Google Scholar]
  • 50.D’Alonzo F., Perilli M., Biagioli M. Nuovi ibridi di Ophrys in Puglia e Basilicata. GIROS Orch. Spont. Eur. 2019;62:161–178. [Google Scholar]
  • 51.Lumare F., Medagli P., Anelli S. Presenza di Barlia robertiana nel Salento leccese e Tarantino. GIROS Orch. Spont. Eur. 2020;63:464–471. [Google Scholar]
  • 52.Lumare F., Medagli P., Turco A., Dura T., Anelli S. Le Orchidacee del Bosco Cuturi. GIROS Orch. Spont. Eur. 2020;63:199–206. [Google Scholar]
  • 53.Lumare F., Medagli P., Anelli S. Le Orchidacee delle Macchie di S. Cataldo (Lecce) GIROS Orch. Spont. Eur. 2020;63:207–217. [Google Scholar]
  • 54.D’Alonzo F., Perilli M., Biagioli M. Due nuovi ibridi garganici di Ophrys sipontensis (Puglia) GIROS Orch. Spont Eur. 2020;63:25–36. [Google Scholar]
  • 55.Lumare F., Medagli P., Anelli S. Osservazioni su una popolazione di Anacamptis collina alle macchie di San Cataldo (Lecce, Salento, Puglia) GIROS Orch. Spont. Eur. 2021;64:87–98. [Google Scholar]
  • 56.Mele C., Caforio F., Marchiori S. Remarkable species occurring in the Alimini Lakes (Apulia South Italy), an area proposed as Site of Community Interest. Bocconea. 2006;19:253–260. [Google Scholar]
  • 57.Turco A., Medagli P. Serapias × marchiorii Turco & Medagli (Serapias bergonii E.G. Camus × Serapias politisii Renz) ibrido naturale nuovo del Salento. Thalass. Salentina. 2009;32:145–150. doi: 10.1285/i15910725v32p145. [DOI] [Google Scholar]
  • 58.Perrino E.V., Signorile G. Costa di Monopoli (Puglia): Checklist della flora vascolare. Inform. Bot. Ital. 2009;41:263–279. [Google Scholar]
  • 59.Tomaselli V., Urbano M., Sciandrello S., Wagensommer R.P., Costanzo E., Albano A., Medagli P., Mele C., Di Pietro R. Cartografia tematica ed analisi del paesaggio vegetale ed agricolo del Parco Naturale Regionale “Saline di Punta della Contessa” (Brindisi—Puglia) Quad. Bot. Amb. Appl. 2010;21:53–76. [Google Scholar]
  • 60.Medagli P., Turco A. Ophrys × rossiniae e Ophrys × quitadamoi, due ibridi naturali nuovi del Gargano. GIROS Not. 2011;47:54–57. [Google Scholar]
  • 61.Dura T., Turco A., Gennaio R., Medagli P. Una nuova entità della Puglia: Ophrys oxyrrhynchos Tod. Subsp. ingrassiae Dura, Turco, Gennaio & Medagli. GIROS Not. 2011;46:37–39. [Google Scholar]
  • 62.Medagli P., Turco A. Serapias × ruggieroi e Serapias × d’emericoi ibridi naturali nuovi del Salento. Thalass. Salentina. 2011;33:75–82. doi: 10.1285/i15910725v33p75. [DOI] [Google Scholar]
  • 63.Turco A., Ruggiero L., Gennaio R., D’Emerico S. Ophrys × medaglii un nuovo ibrido naturale del Salento. Thalass. Salentina. 2012;34:25–32. [Google Scholar]
  • 64.Lumare F., Medagli P. Le orchidee spontanee dello “Stagno di Acquatina” (Lecce) GIROS Not. 2013;52:94–96. [Google Scholar]
  • 65.Medagli P., Sciandrello S., Mele C., Di Pietro R., Wagensommer R.P., Urbano M., Tomaselli V. Analisi della biodiversità vegetale e cartografia della vegetazione, degli Habitat e dell’uso del suolo della Riserva Naturale Statale “Le Cesine” (Lecce-Puglia) Quad. Bot. Amb. Appl. 2013;24:55–76. [Google Scholar]
  • 66.Gennaio R. Nuova segnalazione nel Salento di Orchis italica Poir. GIROS Not. 2014;57:59–61. [Google Scholar]
  • 67.D’Alonzo F. Nuove stazioni di Ophrys gravinensis in Puglia e descrizione di due suoi ibridi. GIROS Orch. Spont. Eur. 2015;58:247–261. [Google Scholar]
  • 68.Dura T., Biagioli M. La presenza di Ophrys exaltata s.l. in provincia di Taranto e in Puglia. GIROS Orch. Spont. Eur. 2016;59:277–285. [Google Scholar]
  • 69.D’Alonzo F., Perilli M. Due nuovi ibridi naturali di Ophrys del Gargano (Puglia) GIROS Orch. Spont. Eur. 2016;59:60–69. [Google Scholar]
  • 70.Gennaio R., Lavarra P. Note preliminari sulla presenza di Orchidaceae nel territorio del Barsento (Bari, Puglia) GIROS Orch. Spont. Eur. 2017;60:71–82. [Google Scholar]
  • 71.Gennaio R. Prima descrizione dell’ibrido naturale Ophrys × barbarae (O. lutea × O. sicula) GIROS Orch. Spont. Eur. 2017;60:421–426. [Google Scholar]
  • 72.Mascia G. Ophrys oxyrrhynchos subsp. ingrassiae, prima segnalazione per la provincia di Brindisi. GIROS Orch. Spont. Eur. 2018;61:87–90. [Google Scholar]
  • 73.Chetta F.S., Gennaio R., Medagli P., Gargiulo M. Prima segnalazione in Italia del raro ibrido intergenerico Anacamptis morio × Serapias cordigera (x Serapicamptis monfortensis comb. nov.) GIROS Orch. Spont. Eur. 2018;61:474–480. [Google Scholar]
  • 74.Fanelli G., Lucchese F., Paura B. Le praterie a Stipa austroitalica di due settori adriatici meridionali (Molise e Gargano) Fitosociologia. 2001;38:25–36. [Google Scholar]
  • 75.Gennaio R. Osservazioni su alcune anomalie fiorali in esemplari di Ophrys apifera Huds. Presso Torre San Giovanni, Ugento (Le) GIROS Not. 2003;22:29–30. [Google Scholar]
  • 76.Gennaio R. Ophrys × ozantina R. Gennaio [Ophrys bobyliflora Link × Ophrys parvimaculata (O. Danesch. & E. Danesch) H.F. Paulus & Gack)], un nuovo ibrido naturale rinvenuto nel Parco Regionale “Litorale di Ugento” (Salento, Puglia) GIROS Not. 2009;41:28–29. [Google Scholar]
  • 77.Tomaselli V., Sciandrello S., Dibitonto P., Wagensommer R.P., Urbano M., Calabrese I.T., Garziano G., Cimmarusti G., Di Pietro R. Analisi del paesaggio vegetale ed agricolo della Riserva Naturale Statale di “Torre Guaceto” (Brindisi Puglia). Cartografia della vegetazione, degli habitat e dell’uso del suolo. Quad. Bot. Amb. Appl. 2010;21:33–49. [Google Scholar]
  • 78.Turco A., Medagli P. Serapias × gennaioi (Serapias cordigera × Serapias orientalis subsp. apulica), ibrido naturale nuovo del Salento. Giros Not. 2012;49:68–70. [Google Scholar]
  • 79.Gennaio R., Lumare F., Medagli P. Nuova segnalazione in Italia dell’ibrido intergenerico × Serapicamptis nouletii (Anacamptis laxiflora × Serapias cordigera) GIROS Orch. Spont. Eur. 2015;58:334–337. [Google Scholar]
  • 80.Beccarisi L., Marinò F., Medagli P., Zizzi T., Minonne F. Inventario della flora vascolare della Riserva Naturale di Torre Guaceto (Puglia) Thalass. Salentina. 2015;37:11–56. doi: 10.1285/i15910725v37p11. [DOI] [Google Scholar]
  • 81.Gennaio R. Serapias apulica subsp. uxentina, un nuovo taxon salentino (Puglia) GIROS Orch. Spont. Eur. 2017;60:347–360. [Google Scholar]
  • 82.Gennaio R., Gargiulo M., Chetta F.S. Serapias × fallax Soó nothosubsp. piccinnoi, nuovo ibrido del Salento tra S. bergonii e S. vomeracea subsp. longipetala. GIROS Orch. Spont. Eur. 2018;61:481–489. [Google Scholar]
  • 83.Gennaio R. Prima segnalazione in Salento dell’ibrido Serapias × broeckii (S. parviflora × S. vomeracea) GIROS Orch. Spont. Eur. 2022;65:36–43. [Google Scholar]
  • 84.Dura T. Il bosco delle Pianelle in provincia di Taranto. GIROS Not. 2004;27:9–13. [Google Scholar]
  • 85.Forte L., Perrino E.V., Terzi M. Le praterie a Stipa austroitalica Martinovsky ssp. austroitalica dell’Alta Murgia (Puglia) e della Murgia Materana (Basilicata) Fitosociologia. 2005;42:83–103. [Google Scholar]
  • 86.Biondi E., Guerra V. Vegetazione e paesaggio vegetale delle gravine dell’Arco jonico. Fitosociologia. 2008;45((Suppl. S1)):57–125. [Google Scholar]
  • 87.Medagli P., Cillo N. Ophrys murgiana Cillo, Medagli & Margherita, specie nuova delle Murge (Puglia, Italia meridionale) GIROS Not. 2009;41:23–25. [Google Scholar]
  • 88.Cillo N. Ophrys mateolana Medagli, D’Emerico, Bianco & Ruggiero, nuove segnalazioni in provincia di Bari. GIROS Not. 2009;40:38–40. [Google Scholar]
  • 89.Liverani V., Romolini R. Ophrys holosericea subsp. paolina, nuova sottospecie del nord del Gargano (Puglia, Italia) J. Eur. Orch. 2010;42:467–476. [Google Scholar]
  • 90.De Leo V. Le Orchidaceae del Parco Naturale di Lama Balice (Bari, Puglia) GIROS Orch. Spont. Eur. 2015;58:129–135. [Google Scholar]
  • 91.D’Alonzo F., Perilli M. Quattro nuovi ibridi naturali di Ophrys nel Parco Nazionale dell’Alta Murgia (Bari, Puglia) GIROS Orch. Spont. Eur. 2016;59:291–310. [Google Scholar]
  • 92.Lumare F., Medagli P. Un nuovo ibrido intergenerico in Salento (Puglia): X Serapicamptis portiferi (Anacamptis morio × Serapias parviflora) GIROS Orch. Spont. Eur. 2016;59:98–106. [Google Scholar]
  • 93.De Leo V. Due importanti ritrovamenti di Ophrys nella Murgia barese: O. crabronifera subsp. pollinensis (prima segnalazione in Puglia) e O. oxyrrhynchos subsp. ingrassiae (prima segnalazione nella provincia di Bari) GIROS Orch. Spont. Eur. 2016;59:329–333. [Google Scholar]
  • 94.Dura T. Ritrovamento presso Taranto (Puglia) del rarissimo ibrido Ophrys × circlarium nothosubsp. circlarium (O. lutea subsp. lutea × O. tarentina) GIROS Orch. Spont. Eur. 2016;59:381–388. [Google Scholar]
  • 95.Lumare F., Medagli P. Studio morfometrico e tassonomico su alcune popolazioni di Serapias apulica (S. orientalis subsp. apulica) in Salento (Puglia) GIROS Orch. Spont. Eur. 2017;60:26–54. [Google Scholar]
  • 96.Gennaio R., Gargiulo M., Chetta F.S. Serapias × watersii nothosubsp. watersii (S. politisii × S. vomeracea subsp. longipetala), nuovo ibrido naturale del Salento (Puglia) GIROS Orch. Spont. Eur. 2017;60:452–460. [Google Scholar]
  • 97.Lumare F., Medagli P., Biagioli M. Serapias × frigolae nothosubsp. nelsonii, ibrido naturale nuovo tra S. apulica subsp. brundisina e S. bergonii. GIROS Orch. Spont. Eur. 2017;60:463–480. [Google Scholar]
  • 98.Lumare F., Medagli P., Dura T., Anelli S. Ophrys × boscocuturiensis, nuovo ibrido triplo del Salento (O. bertolonii subsp. bertolonii × O. holosericea subsp. parvimaculata × O. tenthredinifera subsp. neglecta) GIROS Orch. Spont. Eur. 2018;61:374–386. [Google Scholar]
  • 99.D’Alonzo F., Perilli M., Biagioli M. Un nuovo ibrido naturale di Ophrys nel Parco Nazionale del Gargano (Foggia, Puglia settentrionale) GIROS Orch. Spont. Eur. 2018;61:399–406. [Google Scholar]
  • 100.D’Alonzo F., Perilli M., Biagioli M. Un nuovo ibrido di Ophrys nella Murgia barese (Puglia centrale) tra O. bombyliflora e O. oxyrrhynchos subsp. celiensis. GIROS Orch. Spont. Eur. 2018;61:407–415. [Google Scholar]
  • 101.D’Alonzo F., Perilli M., Biagioli M. Tre nuovi ibridi naturali di Ophrys nel Gargano (Puglia) GIROS Orch. Spont. Eur. 2021;64:66–82. [Google Scholar]
  • 102.Turco A., Albano A., Medagli P., Wagensommer R.P., D’Emerico S. Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae) Comp. Cytogenet. 2021;15:507–525. doi: 10.3897/compcytogen.v15.i4.75990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 103.Forte L., Cavallaro V., Pantaleo F., D’Amico F.S., Macchia F. The vascular Flora of the “Bosco Isola” at Lesina (Foggia—Apulia) Fl. Medit. 2002;12:33–92. [Google Scholar]
  • 104.Beccarisi L., Medagli P., Minonne F., Zuccarello V., Marchiori S. La conservazione della vegetazione nella Riserva di Torre Guaceto. Thalass. Salentina. 2004;27:117–129. [Google Scholar]
  • 105.Musacchio A., Pellegrino G., Cafasso D., Widmer F., Cozzolino S. A unique A. palustris lineage across the Otranto strait: Botanical evidence for a past land-bridge? Plant Syst. Evol. 2006;262:103–111. doi: 10.1007/s00606-006-0469-y. [DOI] [Google Scholar]
  • 106.Di Pietro R., Dibitonto P., Garziano G., Sciandrello S., Wagensommer R.P., Medagli P., Tomaselli V. Preliminary results of floristic and vegetation surveys in three coastal humid areas in the Puglia region (southern Italy) Lazaroa. 2009;30:99–107. [Google Scholar]
  • 107.Serafino S., Chetta F.S., Mascia G. Ophrys × turris-veneris (Ophrys candica × Ophrys classica), un nuovo ibrido del litorale leccese (Salento, Puglia meridionale) GIROS Orch. Spont. Eur. 2022;65:300–311. [Google Scholar]
  • 108.Terzi M., Di Pietro R., D’Amico F.S. Analisi delle Specie Indicatrici applicata alle comunità a Stipa austroitalica Martinovský e relative problematiche sintassonomiche. Fitosociologia. 2010;47:3–28. [Google Scholar]
  • 109.Turco A., D’Emerico S., Lozito L., Medagli P. Ophrys peucetiae e Ophrys pseudomelena, due nuovi taxa per la Puglia. Giros Not. 2012;50:4–11. [Google Scholar]
  • 110.Gennaio R., Medagli P. Ophrys × sulphurea Gennaio & Medagli, nuovo ibrido naturale del Salento (Puglia) GIROS Orch. Spont. Eur. 2015;58:110–113. [Google Scholar]
  • 111.D’Alonzo F. Un nuovo ibrido di Ophrys in Puglia: Ophrys × michelae D’Alonzo (O. oxyrrhynchos subsp. celiensis × O. tenthredinifera subsp. neglecta) GIROS Orch. Spont. Eur. 2015;58:279–285. [Google Scholar]
  • 112.Lumare F., Medagli P., Dura T., Serafino S., Anelli S., Biagioli M. Presenza sulla costa adriatica leccese di Ophrys tarentina e del suo ibrido con O. passionis subsp. garganica (Salento, Puglia meridionale) GIROS Orch. Spont. Eur. 2018;61:490–497. [Google Scholar]
  • 113.D’Alonzo F., Leone F., Biagioli M. Ophrys speculum nel Parco Nazionale dell’Alta Murgia (Bari, Puglia) GIROS Orch. Spont. Eur. 2021;64:278–281. [Google Scholar]
  • 114.D’Alonzo F. Ophrys gravinensis, nuova specie endemica della Puglia. GIROS Orch. Spont. Eur. 2015;58:98–106. [Google Scholar]
  • 115.Gennaio R., Gargiulo M., Medagli P., Chetta F.S. Ophrys × sivana nothosubsp. renatafontae (O. candica × O. holosericea subsp. parvimaculata), nuovo ibrido naturale del Salento (Puglia) GIROS Orch. Spont. Eur. 2016;59:415–420. [Google Scholar]
  • 116.Perilli M. Prima segnalazione di Orchis simia per il Gargano e la provincia di Foggia (Puglia) GIROS Orch. Spont. Eur. 2017;60:117–118. [Google Scholar]
  • 117.Santoro G. Nuove segnalazioni di Orchidaceae sui monti Dauni (Foggia) GIROS Orch. Spont. Eur. 2017;60:147–150. [Google Scholar]
  • 118.Gennaio R., Gargiulo M., Medagli P., Chetta F.S. Ophrys × costae (O. fusca subsp. lupercalis × O. sicula), nuovo ibrido naturale del Salento (Puglia) GIROS Orch. Spont. Eur. 2018;61:53–59. [Google Scholar]
  • 119.Perilli M., Biagioli M. Tre nuovi ibridi di Ophrys nel Gargano (Puglia) GIROS Orch.Spont. Eur. 2020;63:500–515. [Google Scholar]
  • 120.Wagensommer R.P., Marrese M., Perrino E.V., Bartolucci F., Cancellieri L., Carruggio F., Conti F., Di Pietro R., Fortini P., Galasso G., et al. Contributo alla conoscenza floristica della Puglia: Resoconto dell’escursione del Gruppo di Floristica (S.B.I.) nel 2011 nel settore meridionale dei Monti della Daunia. Inform. Bot. Ital. 2014;46:175–208. [Google Scholar]
  • 121.Palladino L., Russo G. Primo ritrovamento in Puglia di Epipogium aphyllum Sw. GIROS Orch. Spont. Eur. 2017;60:302–307. [Google Scholar]
  • 122.D’Emerico S., Cozzolino S., Pellegrino G., Pignone D., Scrugli A. Karyotype structure, supernumerary chromosomes and heterochromatin distribution suggest a pathway of karyotype evolution in Dactylorhiza (Orchidaceae) Bot. J. Linn. Soc. 2002;138:85–91. doi: 10.1046/j.1095-8339.2002.00008.x. [DOI] [Google Scholar]
  • 123.Rossini A., Quitadamo G., Turco A., Medagli P. Epipactis persica subsp. gracilis (B. Baumann & H. Baumann) W. Rossi ed Epipactis purpurata Sm., specie nuove per il Gargano e per la Puglia. GIROS Not. 2013;52:107–108. [Google Scholar]
  • 124.Hertel S., Presser H. Weitere Erkenntnisse zur Vielfalt der Epipactis-Arten im südlichen Italien. Ber. Aus Den Arb. Heim. Orchid. 2019;36:5–61. [Google Scholar]
  • 125.Rossini A., Quitadamo G. Epipactis leptochila (Godfery) Godfery var. neglecta (H. Kumpel) A. Gevaudan. GIROS Not. 2008;39:26–27. [Google Scholar]
  • 126.Dura T. La Gravina di Alezza nel Parco Naturale Regionale “Terra delle Gravine”, sito del primo ritrovamento di Epipactis helleborine in provincia di Taranto (Puglia) GIROS Not. 2010;43:21–27. [Google Scholar]
  • 127.Kreutz K., Rossini A., Quitadamo G., Turco A., Medagli P. Epipactis leptochila (Godfery) subsp. umbrae (Fam. Orchidaceae) nuovo taxon della Puglia. Thalass. Salentina. 2014;36:79–90. doi: 10.1285/i15910725v36p79. [DOI] [Google Scholar]
  • 128.Gennaio R., Bongiorni L. Prima segnalazione di Epipactis helleborine nel Salento leccese all’Arboreto Francigeno di Tricase (Puglia) GIROS Orch. Spont. Eur. 2018;61:318–325. [Google Scholar]
  • 129.De Matteis G., Gennaio R., Leopizzi A. Segnalazione di una nuova stazione di Epipactis microphylla (Ehrh.), specie rara per il Salento, rinvenuta nella Riserva Naturale dello Stato “Le Cesine” (Vernole, Lecce) GIROS Not. 2009;42:14–16. [Google Scholar]
  • 130.Del Fuoco C., Scirocco T. Epipactis palustris (L.) Cranz: Nuova segnalazione nel Gargano. GIROS Not. 2002;20:8. [Google Scholar]
  • 131.Rambelli A., Ciccarone C., Tempesta S., Venturella G. Dematiaceous Hyphomycetes from Bosco Isola (S Italy) Mediterranean maquis litter. Fl. Medit. 2010;20:239–258. [Google Scholar]
  • 132.Perilli M. Ophrys holosericea subsp. gracilis (Buel, O. Danesch & E. Danesch) Buel, O. Danesch & E. Danesch, prima segnalazione per la Puglia. GIROS Not. 2009;42:19. [Google Scholar]
  • 133.Santoro G. Nuove segnalazioni di Orchidaceae nei Monti Dauni Meridionali (Bovino, Foggia) GIROS Notizie. 2010;45:34–35. [Google Scholar]
  • 134.Biondi E., Casavecchia S., Guerra V., Medagli P., Beccarisi L., Zuccarello V. A contribution towards the knowledge of semideciduous and evergreen woods of Apulia (south-eastern Italy) Fitosociologia. 2004;41:3–28. [Google Scholar]
  • 135.Dura T. Orchis ustulata in provincia di Taranto. GIROS Not. 2002;21:24–25. [Google Scholar]
  • 136.Dura T. Neottia nidus-avis (L.) Rich. Prima segnalazione per la provincia di Taranto. GIROS Not. 2004;27:26–27. [Google Scholar]
  • 137.Del Fuoco C. Ophrys funerea Viviani. Nuova Stazione nel Gargano. GIROS Not. 2002;21:20–21. [Google Scholar]
  • 138.D’Emerico S., Pignone D., Bartolo G., Pulvirenti S., Terrasi C., Stuto S., Scrugli A. Karyomorphology, heterochromatin patterns and evolution in the genus Ophrys (Orchidaceae) Bot. J. Linn. Soc. 2005;148:87–99. doi: 10.1111/j.1095-8339.2005.00393.x. [DOI] [Google Scholar]
  • 139.D’Alonzo F., Perilli M., Biagioli M. Nuovi ibridi di Ophrys nella Murgia barese (Puglia meridionale) GIROS Orch. Spont. Eur. 2020;63:322–337. [Google Scholar]
  • 140.Perilli M. Cinque nuovi ibridi naturali di Ophrys del Gargano (Puglia) GIROS Orch. Spont. Eur. 2015;58:309–316. [Google Scholar]
  • 141.Turco A., Albano A., Medagli P., D’Emerico S. A cytological study on Ophrys (Orchidaceae) in Italy: New evidence and the importance of polyploidy. Plant Biosyst. 2015;149:24–30. doi: 10.1080/11263504.2013.809028. [DOI] [Google Scholar]
  • 142.Gennaio R., Gargiulo M., Medagli P., Chetta F.S. Ophrys × montalciniae (O. incubacea subsp. incubacea × O. sphegodes subsp. classica), nuovo ibrido naturale del Salento (Puglia) GIROS Orch. Spont. Eur. 2016;59:339–346. [Google Scholar]
  • 143.Serafino S., Gennaio R., Chetta F.S., Gargiulo M., Medagli P. Ophrys × salapiensis (O. apulica × O. bombyliflora × O. candica), un nuovo ibrido triplo del Salento. GIROS Orch. Spont. Eur. 2017;60:438–446. [Google Scholar]
  • 144.D’Alonzo F., Perilli F. Quattro nuovi ibridi naturali di Ophrys del Gargano (Foggia, Puglia) GIROS Orch. Spont. Eur. 2017;60:5–25. [Google Scholar]
  • 145.Chetta F.S., Gennaio R., Gargiulo M., Serafino S., Medagli P. Ritrovamento nel Salento leccese di Ophrys speculum dopo 131 anni dalla prima segnalazione di Henry Groves. GIROS Orch. Spont. Eur. 2018;61:429–433. [Google Scholar]
  • 146.Lumare F., Medagli P., Biagioli M. Nuovo rinvenimento e studio morfologico del raro ibrido salentino Ophrys × hydruntensis (O. apulica × O. tardans) GIROS Orch. Spont. Eur. 2018;61:41–52. [Google Scholar]
  • 147.Lumare F., Medagli P., Dura T., Anelli S. Ophrys holosericea subsp. parvimaculata: Una nuova forma (f. chloranta) e un nuovo ibrido con Ophrys lutea (O. × pulcherrima) GIROS Orch. Spont. Eur. 2019;62:90–100. [Google Scholar]
  • 148.Chetta F.S., Gennaio R., Gargiulo M., Serafino S. Presenza di Ophrys tarentina lungo la costa ionica leccese (Salento, Puglia meridionale) GIROS Orch. Spont. Eur. 2020;63:316–321. [Google Scholar]
  • 149.Gennaio R. Prima segnalazione di Ophrys sipontensis nel Salento meridionale (Lecce, Puglia) GIROS Orch. Spont. Eur. 2022;65:252–255. [Google Scholar]
  • 150.Serafino S., Mascia G., Chetta F.S. Una popolazione di Ophrys tarentina nel Salento leccese (Puglia meridionale) GIROS Orch. Spont Eur. 2022;65:312–317. [Google Scholar]
  • 151.Gennaio R., Gargiulo M., Medagli P., Chetta F.S. Ophrys × angelarum (O. bertolonii × O. tardans), nuovo ibrido naturale del Salento (Puglia) GIROS Orch. Spont. Eur. 2016;59:334–338. [Google Scholar]
  • 152.Lumare F., Medagli P., Dura T., Anelli S. Studio morfologico su una popolazione di Ophrys × salentina (O. holosericea subsp. apulica × O. tenthredinifera subsp. neglecta) GIROS Orch. Spont. Eur. 2019;62:123–132. [Google Scholar]
  • 153.Lumare F., Medagli P., Anelli S. Un nuovo morfotipo dell’ibrido Ophrys × angelarum (O. bertolonii × O. tardans) GIROS Orch. Spont. Eur. 2020;63:163–170. [Google Scholar]
  • 154.Perilli M. Un nuovo ibrido di Ophrys del Gargano (Puglia) GIROS Orch. Spont. Eur. 2016;59:170–174. [Google Scholar]
  • 155.D’Alonzo F., Perilli M., Biagioli M. Tre nuovi ibridi naturali di Ophrys nel Parco Nazionale del Gargano (Foggia, Puglia settentrionale) GIROS Orch.Spont. Eur. 2017;60:308–321. [Google Scholar]
  • 156.Devey D.S., Bateman R.M., Fay F.M., Hawkins A.J. Friends or relatives? Phylogenetics and species delimitation in the controversial European orchid genus Ophrys. Ann. Bot. 2008;101:385–402. doi: 10.1093/aob/mcm299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 157.Perilli M. Tre nuovi ibridi naturali di Ophrys del Gargano. Giros Notizie. 2012;51:108–110. [Google Scholar]
  • 158.Gennaio R., Gargiulo M., Medagli P., Chetta F.S. Due nuovi ibridi di Ophrys incubacea subsp. brutia nel Salento. GIROS Orch. Spont. Eur. 2016;59:389–395. [Google Scholar]
  • 159.Lumare F., Medagli P. Ophrys × biagiolii, nuovo ibrido triplo del Salento (O. bombyliflora × O. candica × O. holosericea subsp. parvimaculata) GIROS Orch. Spont. Eur. 2018;61:363–373. [Google Scholar]
  • 160.Gennaio R., Medagli P. Un nuovo triplo ibrido di Ophrys nel Salento (Puglia): O. bombyliflora × O. passionis subsp. garganica × O. sphegodes subsp. classica. GIROS Orch. Spont. Eur. 2019;62:364–370. [Google Scholar]
  • 161.Gennaio R. Ophrys iricolor subspp. eleonorae e lojaconoi, prime segnalazioni per il Salento (Puglia) GIROS Orch. Spont. Eur. 2016;59:311–315. [Google Scholar]
  • 162.Gennaio R., Gargiulo M., Medagli P., Chetta F.S. Ophrys × montalciniae nothosubsp. cristoforettiae (O. incubacea subsp. brutia × O. sphegodes subsp. classica), nuovo ibrido naturale del Salento (Puglia) GIROS Orch. Spont. Eur. 2017;60:427–431. [Google Scholar]
  • 163.Mascia G., Labadessa R. La presenza in Puglia di Ophrys fusca subsp. calocaerina. GIROS Orch. Spont. Eur. 2019;62:36–40. [Google Scholar]
  • 164.Lumare F., Medagli P. Ritrovamento di un nuovo ibrido naturale nel Salento: Ophrys × silvanae (O. candica × O. tardans) GIROS Orch. Spont. Eur. 2017;60:481–494. [Google Scholar]
  • 165.Lumare F., Medagli P. Identificazione dell’ibrido occasionale tra Ophrys candica e O. tenthredinifera subsp. neglecta in Salento e suo confronto con O. tardans. GIROS Orch. Spont. Eur. 2018;61:20–27. [Google Scholar]
  • 166.Lumare F., Medagli P., Anelli S. Variabilità dei caratteri fiorali in una popolazione di O. candica. GIROS Orch. Spont. Eur. 2020;63:265–283. [Google Scholar]
  • 167.Gennaio R. Ophrys celiensis (O. Danesch & E. Danesch) P. Delforge, prima segnalazione per il Salento. GIROS Not. 2008;38:44–45. [Google Scholar]
  • 168.Rossini A., Quitadamo G. Ophrys conradiae Melki & Deschatres, prima segnalazione per il Gargano e la Puglia. Giros Not. 2007;36:37–38. [Google Scholar]
  • 169.D’Alonzo F. Ophrys conradiae Melki & Deschatres, nuova segnalazione per la Puglia. GIROS Not. 2009;42:11–12. [Google Scholar]
  • 170.Campochiaro M., Dura T. Ophrys scolopax subsp. conradiae (Melki & Deschatres) H Baumann, Giotta, Künkele, R. Lorenz & Piccitto, prima segnalazione per la provincia di Taranto. GIROS Not. 2011;48:42–43. [Google Scholar]
  • 171.Del Fuoco C. Ophrys lutea subsp. phryganae (Devillers-Tersch. & Devillers) Melki. Nuova Stazione nel Gargano. GIROS Not. 2002;21:21. [Google Scholar]
  • 172.Turco A., Medagli P., Wagensommer R.P., D’Emerico S., Gennaio R., Albano A. A morphometric study on Ophrys sect. Pseudophrys in Apulia (Italy) and discovery of Ophrys japigiae sp. nov. (Orchidaceae) Plant Biosyst. 2021;156:560–571. doi: 10.1080/11263504.2021.1897702. [DOI] [Google Scholar]
  • 173.Turco A., Albano A., Medagli P., Pulvirenti S., D’Emerico S. New cytological data in Ophrys sect. Pseudophrys Godfery and comparative karyomorphological studies in Ophrys L. (Orchidaceae) Plant Biosyst. 2017;152:901–910. doi: 10.1080/11263504.2017.1362058. [DOI] [Google Scholar]
  • 174.Perilli M. Alcune considerazioni sugli individui di Ophrys lacaitae con fiori a labello scuro. GIROS Orch. Spont. Eur. 2015;58:343–348. [Google Scholar]
  • 175.Gennaio R., Gargiulo M., Chetta F.S., Medagli P. Ophrys × pratesii nothosubsp. pratesii (O. fusca subsp. lupercalis × O. iricolor subsp. lojaconoi), nuovo ibrido naturale del Salento (Puglia meridionale) GIROS Orch. Spont. Eur. 2017;60:432–437. [Google Scholar]
  • 176.Perilli M. Entità del gruppo Oprhys fusca nel Gargano, con la prima segnalazione di Ophrys fusca subsp. lucana. GIROS Not. 2010;45:31–32. [Google Scholar]
  • 177.Medagli P., Rossini A., Quitadamo G., D’Emerico S., Turco A. Ophrys mattinatae, specie nuova del Gargano. Giros Not. 2012;51:102–103. [Google Scholar]
  • 178.Van de Vijver B., Van Looken W., Thiers G., Cuypers A. Ophrys oestrifera subsp. montis-gargani, a new subspecies from the Gargano (Italy) J. Eur. Orch. 2010;42:167–180. [Google Scholar]
  • 179.Serafino S. Ophrys xsancti-ligorii (Ophrys incubacea × Ophrys tardans) un nuovo ibrido presso Lecce (Salento, Puglia merdionale) GIROS Orch. Spont. Eur. 2022;65:290–299. [Google Scholar]
  • 180.Gennaio R., Gargiulo M., Biagioli M. Prima segnalazione per l’Italia peninsulare (Puglia) di Ophrys panormitana. GIROS Orch. Spont. Eur. 2022;65:29–35. [Google Scholar]
  • 181.Lumare F., Medagli P., Wagensommer R.P., Dura T., Anelli S. La variabilità dei caratteri fiorali in una popolazione di Ophrys holosericea subsp. parvimaculata. GIROS Orch. Spont. Eur. 2019;62:270–284. [Google Scholar]
  • 182.Perilli M. Ophrys pinguis Romolini & Soca al Gargano, prima segnalazione per la Puglia. Orch. Spont. Eur. 2015;58:153. [Google Scholar]
  • 183.Gennaio R., Manni Q. Nuova segnalazione di Ophrys speculum nel Salento leccese (Puglia meridionale) GIROS Orch. Spont. Eur. 2022;65:44–47. [Google Scholar]
  • 184.Lumare F., Medagli P. Su alcune caratteristiche morfologiche di importanza tassonomica in Ophrys tardans. GIROS Orch. Spont. Eur. 2018;61:11–19. [Google Scholar]
  • 185.Lumare F., Medagli P., Anelli S. Il nuovo ibrido Ophrys × lizzanensis (O. bombyliflora × O. tardans) a confronto con O. × sommieri (O. bombyliflora × O. tenthredinifera) GIROS Orch. Spont. Eur. 2019;62:300–315. [Google Scholar]
  • 186.Del Fuoco C. Nuove segnalazioni di Ophrys nel Gargano: Ophrys tarquinia P. Delforge. Tre ibridi e un lusus. GIROS Not. 2008;38:36–39. [Google Scholar]
  • 187.D’Agostino V., Liuzzi C. Orchis simia prima segnalazione per la Puglia. GIROS Not. 2010;43:52–53. [Google Scholar]
  • 188.Del Fuoco C., Pantaleo F. Platanthera bifolia (L.) Rich.: Prime segnalazioni documentate nel Gargano. GIROS Not. 2002;20:14–15. [Google Scholar]
  • 189.Turco A., Albano A., Medagli P., D’Emerico S. Contribution to the study of wild Orchidaceae, genus Platanthera L.C.M. Richard. Karyotype and C-banding analysis of two species from Italy. Caryologia. 2020;73:11–16. doi: 10.13128/caryologia-811. [DOI] [Google Scholar]
  • 190.Gennaio R., Medagli P. Prima segnalazione per il Salento (Puglia) di Serapias × kelleri Camus (Serapias cordigera L. × S. vomeracea (Burm. F.) Briq. subsp. vomeracea) GIROS Not. 2010;45:14–16. [Google Scholar]
  • 191.Perrino E.V., Signorile G., Marvulli M. A first checklist of the vascular flora of the Polignano a Mare coast (Apulia, southern Italy) Natura Croatica. 2013;22:295–318. [Google Scholar]
  • 192.Lumare F., Medagli P. Serapias × barsellae, nuovo ibrido naturale tra S. bergonii e S. parviflora. GIROS Orch. Spont. Eur. 2015;58:40–49. [Google Scholar]
  • 193.Lumare F., Medagli P. Primo rinvenimento in Italia (Puglia) dell’ibrido Serapias × demadesii (S. bergonii × S. lingua) GIROS Orch. Spont. Eur. 2015;58:50–57. [Google Scholar]
  • 194.Lumare F., Medagli P. Rinvenimento di una nuova popolazione ibridogena stabilizzata di Serapias (Serapias ambigua subsp. sallentina, subsp. nova) in Salento (Puglia) GIROS Orch. Spont. Eur. 2015;58:290–308. [Google Scholar]
  • 195.Lumare F., Medagli P., Zupo V. Studio morfometrico su una popolazione di Serapias orientalis subsp. apulica presso Lecce (Salento, Puglia) GIROS Orch. Spont. Eur. 2016;59:107–119. [Google Scholar]
  • 196.Lumare F., Medagli P. Studio morfometrico su una popolazione di Serapias × intermedia (S. lingua × S. vomeracea subsp. vomeracea) presso Lecce (Salento, Puglia) GIROS Orch. Spont. Eur. 2016;59:120–131. [Google Scholar]
  • 197.Lumare F., Medagli P. Due nuovi ibridi di Serapias orientalis subsp. apulica in Salento (Puglia), uno con S. lingua e uno con S. bergonii. GIROS Orch. Spont. Eur. 2016;59:150–164. [Google Scholar]
  • 198.Lumare F., Medagli P. Prima segnalazione per il Salento (Puglia) dell’ibrido naturale Serapias × halacsyana (S. bergonii × S. cordigera) GIROS Orch. Spont. Eur. 2016;59:185–195. [Google Scholar]
  • 199.Lumare F., Medagli P. Segnalazione dell’ibrido Serapias × garganica nothosubsp. garganica (S. orientalis susbp. apulica × S. vomeracea subsp. longipetala) presso Lecce (Salento, Puglia) GIROS Orch. Spont. Eur. 2016;59:196–204. [Google Scholar]
  • 200.Lumare F., Medagli P. Ritrovamento del raro ibrido Serapias × rainei (S. cordigera × S. parviflora) in Salento (Puglia) GIROS Orch. Spont. Eur. 2017;60:123–138. [Google Scholar]
  • 201.Lumare F., Medagli P., Biagioli M. Studio morfologico e morfometrico su una popolazione stabilizzata di origine ibridogena da Serapias cordigera e S. vomeracea subsp. longipetala presso Lecce (Salento, Puglia), con descrizione di una nuova microspecie (Serapias guadinae) GIROS Orch. Spont. Eur. 2017;60:193–213. [Google Scholar]
  • 202.Lumare F., Medagli P., Rossini A., Quitadamo G. Studio di approfondimento su alcune sottospecie pugliesi di Serapias apulica. GIROS Orch. Spont. Eur. 2018;61:444–459. [Google Scholar]
  • 203.Gennaio R., Pellegrino G. Serapias ausoniae (Orchidaceae; Orchideae): A new species from southern Italy confirmed by morphological, cytological and molecular analyses. Phytotaxa. 2021;516:159–168. doi: 10.11646/phytotaxa.516.2.3. [DOI] [Google Scholar]
  • 204.Lumare F., Medagli P. Serapias × carluccioi, nuovo ibrido salentino tra S. ambigua subsp. sallentina e S. politisii. GIROS Orch. Spont. Eur. 2018;61:121–135. [Google Scholar]
  • 205.Bellusci F., Musacchio A., Palermo A.M., Pellegrino G. Exploring the history of Serapias politisii (Orchidaceae), a narrow endemic on the opposite coasts of the Otranto channel: Insights from molecular investigations. Bot. J. Linn. Soc. 2010;162:572–580. doi: 10.1111/j.1095-8339.2010.01035.x. [DOI] [Google Scholar]
  • 206.Lumare F., Rossini A., Quitadamo G., Gennaio R., Medagli P. La presenza in Puglia di Serapias vomeracea subsp. vomeracea. GIROS Orch.Spont. Eur. 2015;58:25–39. [Google Scholar]
  • 207.Lumare F., Rossini A., Quitadamo G., Gennaio R., Medagli P., Zupo V. Variabilità nelle popolazioni di Serapias vomeracea subsp. vomeracea in Puglia. GIROS Orch. Spont. Eur. 2016;59:35–44. [Google Scholar]
  • 208.Lumare F., Rossini A., Quitadamo G., Gennaio R., Medagli P., Zupo V. Caratteri morfologici distintivi di Serapias vomeracea subsp. longipetala (Orchidaceae) in Puglia. GIROS Orch. Spont. Eur. 2015;58:317–333. [Google Scholar]
  • 209.Pezzetta A. Le orchidee della fora italiana: Distribuzione geografica e origini. GIROS Orch. Spont. Eur. 2018;61:218–248. [Google Scholar]
  • 210.Bateman R.M., Rudall P.J. Morphological Continua Make Poor Species: Genus-Wide Morphometric Survey of the European Bee Orchids (Ophrys L.) Biology. 2023;12:136. doi: 10.3390/biology12010136. [DOI] [PMC free article] [PubMed] [Google Scholar]

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