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. 2023 May 3;42(24):2007–2016. doi: 10.1038/s41388-023-02706-6

Fig. 3. Everolimus induces innate immune cell infiltration in the polyps.

Fig. 3

APCMin/+ mice with established polyps were treated with Everolimus for 3 days or 14 days (Fig. 1A). The intestinal polyps were analyzed. A Representative CD3ε and CD8 IF staining in the polyps. Bar = 100 µm. B Quantitation of cells per 400× field from (A). C Representative Ly-6B.2 and CD68 IF staining. Higher magnification images are shown in insets (A, C). D Quantitation of cells per 400× field from (D). E qRT-PCR analysis of the indicated immune genes. cDNA was made from pooled polyps, 3–5 polyps/mouse, and 3 mice/group. B, D, E, n = 3 mice/group. *P < 0.05, ***P < 0.001 (Student’s T-Test, two-tailed). +++P < 0.001 (one-way ANOVA and Tukey Post-Hoc test).