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. 2023 Jun 9;12:e72681. doi: 10.7554/eLife.72681

Figure 2. Xin actin binding repeat containing 2 (XIRP2) is enriched at gaps in stereocilia F-actin cores.

Figure 2.

(A) XIRP2 immunostaining is enriched at noise-induced gaps in phalloidin staining in inner hair cells (IHCs) (yellow arrows). (B) XIRP2 immunostaining is enriched at naturally occurring phalloidin-negative gaps in utricle hair cells (yellow arrows). (C) Cartoon and representative image (1×5 μm) of XIRP2 enrichment throughout length of gap in phalloidin signal. (D) Line scan of fluorescence intensity in phalloidin (green) and XIRP2 (magenta) channels along the length of a gap in which XIRP2 is enriched throughout the gap. (E) Cartoon and representative image (1×5 μm) of XIRP2 enrichment at the edges of a gap. (F) Line scan of fluorescence intensity in phalloidin (green) and XIRP2 (magenta) channels in which XIRP2 is only enriched at the edges of the gap. Images are representative of >10 experiments.