XIRP2constructs | Sequence | |
---|---|---|
NTD (incl. LIM) | Forward primer | GGATCCACCGGATCTAGATAACTGATCATAATCAGCCATACC |
Reverse primer | AGACTTAAAAAGTTGTTTAAAATGAGGCTTGCAGTATATTCGTCC | |
Resulting peptide sequence | MADNLEPSTFPIQKGSLSRLRQKWESSDCQRNESYPGGSRCKLFQL KESNLLEPEGEALLTLDPPEAPSLPCCKKEEVLCGEPEDGVPEDQND KLKDCGQPEVLKEDSLTGRRRIERFSIALDELRSIFESPKSSINSAGPV EYVQKEVEIGRSLCSPTFRSLPGSHADDFMRDSDLKSEEVPLDKMSP KSGQSPSMEVTFNLTKPGDVSTAGSEDQSDLLEALSLKERMARYQAA VSRGDTRSFSANVMEESDVCTVPGGLAKMKRQFEKDKMTSTCNAFSE YQYRHESRAEQEAIHSSQEIIRRNEQEVSKGHGTDVFKAEMMSHLEK HTEETKQASQFHQYVQETVIDSPEEEELPKVSTKILKEQFEKSAQENF LRSDKETSPPAKCMKKLLVQDKEICIICQKTVYPMECLIADKQNFHKS CFRCHHCSSKLSLGNYASLHGRIYCKPHFKQLFKS |
|
CTD | Forward primer | TTTGGACACAAGCAACATAAAGATCGATGGAATTGCAAAAAC |
Reverse primer | AGCTCGAGATCTGAGTCCGGACTTGTACAGCTCGT | |
Resulting peptide sequence | KGNYDEGFGHKQHKDRWNCKNQSSLVDFIPSGEPDAHENPT ADTLLLGDLTTHPDACNSKRQDNDLRKWGDRGKLKIVWPPC QEMPKKNSPPEEEFKVNKAKWPPEVTIPVPSEFKRESLTEHVK TLESQGQEQDSVPDLQPCKHVCQKEDITGIKEIKGYEERKDEK EAKDTLKDAEGLRSKRKSGMEFNDHNAHAQSDGKEKNALVN EADSADVLQVANTDDEGGPENHRENFNNNNNNNSVAVSSLN NGRRKISISERPRLLQAVSEANYYTSEYQIKNFNNASKISELLGI FESQKLSSKKVLALALERTADRGTAGSPLQLVLEPGLQQGFSVK GENLAASPDVSPLHIKGNHENNKNVHLFFSNTVKITSFSKKHNI LGCDVMDSVDQLKNMSCLYLRELGKNVKCWHGETAGAARHG GKMCFDAQSQGSAAKPVFPSMQCQTQHLTVEEQIKRDRCYSDSEAD* |
|
LIM only (generated from NTD) | Forward primer | GAAATTTGCATAATTTGTCAAAAGACAGTTTATCCAATGGAGTGC |
Reverse primer | AGCTCGAGATCTGAGTCCGGACTTGTACAGCTCGT | |
Resulting peptide sequence | EICIICQKTVYPMECLIADKQNFHKSCFRCHHCSSKLSLGNYASLHGRIYCKPHFKQLFKS |