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. 2023 Jun 9;12:e72681. doi: 10.7554/eLife.72681
XIRP2constructs Sequence
NTD (incl. LIM) Forward primer GGATCCACCGGATCTAGATAACTGATCATAATCAGCCATACC
Reverse primer AGACTTAAAAAGTTGTTTAAAATGAGGCTTGCAGTATATTCGTCC
Resulting peptide sequence MADNLEPSTFPIQKGSLSRLRQKWESSDCQRNESYPGGSRCKLFQL
KESNLLEPEGEALLTLDPPEAPSLPCCKKEEVLCGEPEDGVPEDQND
KLKDCGQPEVLKEDSLTGRRRIERFSIALDELRSIFESPKSSINSAGPV
EYVQKEVEIGRSLCSPTFRSLPGSHADDFMRDSDLKSEEVPLDKMSP
KSGQSPSMEVTFNLTKPGDVSTAGSEDQSDLLEALSLKERMARYQAA
VSRGDTRSFSANVMEESDVCTVPGGLAKMKRQFEKDKMTSTCNAFSE
YQYRHESRAEQEAIHSSQEIIRRNEQEVSKGHGTDVFKAEMMSHLEK
HTEETKQASQFHQYVQETVIDSPEEEELPKVSTKILKEQFEKSAQENF
LRSDKETSPPAKCMKKLLVQDKEICIICQKTVYPMECLIADKQNFHKS
CFRCHHCSSKLSLGNYASLHGRIYCKPHFKQLFKS
CTD Forward primer TTTGGACACAAGCAACATAAAGATCGATGGAATTGCAAAAAC
Reverse primer AGCTCGAGATCTGAGTCCGGACTTGTACAGCTCGT
Resulting peptide sequence KGNYDEGFGHKQHKDRWNCKNQSSLVDFIPSGEPDAHENPT
ADTLLLGDLTTHPDACNSKRQDNDLRKWGDRGKLKIVWPPC
QEMPKKNSPPEEEFKVNKAKWPPEVTIPVPSEFKRESLTEHVK
TLESQGQEQDSVPDLQPCKHVCQKEDITGIKEIKGYEERKDEK
EAKDTLKDAEGLRSKRKSGMEFNDHNAHAQSDGKEKNALVN
EADSADVLQVANTDDEGGPENHRENFNNNNNNNSVAVSSLN
NGRRKISISERPRLLQAVSEANYYTSEYQIKNFNNASKISELLGI
FESQKLSSKKVLALALERTADRGTAGSPLQLVLEPGLQQGFSVK
GENLAASPDVSPLHIKGNHENNKNVHLFFSNTVKITSFSKKHNI
LGCDVMDSVDQLKNMSCLYLRELGKNVKCWHGETAGAARHG
GKMCFDAQSQGSAAKPVFPSMQCQTQHLTVEEQIKRDRCYSDSEAD*
LIM only (generated from NTD) Forward primer GAAATTTGCATAATTTGTCAAAAGACAGTTTATCCAATGGAGTGC
Reverse primer AGCTCGAGATCTGAGTCCGGACTTGTACAGCTCGT
Resulting peptide sequence EICIICQKTVYPMECLIADKQNFHKSCFRCHHCSSKLSLGNYASLHGRIYCKPHFKQLFKS