Skip to main content
NCBI home page
Gut logoLink to Gut
. 1997 Jan;40(1):14–19. doi: 10.1136/gut.40.1.14

Effect of N alpha-methyl-histamine on acid secretion in isolated cultured rabbit parietal cells: implications for Helicobacter pylori associated gastritis and gastric physiology.

I L Beales 1, J Calam 1
PMCID: PMC1027001  PMID: 9155569

Abstract

BACKGROUND: Helicobacter pylori has been shown to produce the unusual metabolite N alpha-methyl-histamine. This compound is known to be a potent agonist at inhibitory histamine H3 receptors. There is increasing evidence implicating this receptor in the control of gastric acid secretion but the mechanism for this remains to be clarified. AIMS: To investigate the effect of N alpha-methyl-histamine on the acid secretory activity of parietal cells and to determine the mechanism for such effects, thus helping to determine the role of this compound in the pathophysiology of H pylori infection. METHODS: Rabbit parietal cells were isolated and enriched by collagenase-EDTA digestion and centrifugal elutriation. Following culture on Matrigel coated plates, acid secretion was assessed by 14C aminopyrine accumulation. RESULTS: N alpha-methyl-histamine (100 microM) was as potent as histamine (100 microM) in stimulating acid secretion. This effect was reversed by ranitidine indicating it was mediated via the H2 receptor. N alpha-methyl-histamine potentiated the effects of both carbachol (increased by 280%) and gastrin (by 350%) (p < 0.01). N alpha-methyl-histamine had no inhibitory actions on forskolin or carbachol stimulated acid secretion suggesting that there is not an inhibitory H3 receptor located directly on the parietal cell. CONCLUSIONS: Bacterially produced N alpha-methyl-histamine directly stimulates acid secretion by parietal cells and this may contribute to the increased acid secretion that contributes to duodenal ulceration.

Full text

PDF
14

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arrang J. M., Garbarg M., Schwartz J. C. Auto-inhibition of brain histamine release mediated by a novel class (H3) of histamine receptor. Nature. 1983 Apr 28;302(5911):832–837. doi: 10.1038/302832a0. [DOI] [PubMed] [Google Scholar]
  2. Berglindh T., Helander H. F., Obrink K. J. Effects of secretagogues on oxygen consumption, aminopyrine accumulation and morphology in isolated gastric glands. Acta Physiol Scand. 1976 Aug;97(4):401–414. doi: 10.1111/j.1748-1716.1976.tb10281.x. [DOI] [PubMed] [Google Scholar]
  3. Bertaccini G., Coruzzi G. An update on histamine H3 receptors and gastrointestinal functions. Dig Dis Sci. 1995 Sep;40(9):2052–2063. doi: 10.1007/BF02208678. [DOI] [PubMed] [Google Scholar]
  4. Black J. W., Duncan W. A., Durant C. J., Ganellin C. R., Parsons E. M. Definition and antagonism of histamine H 2 -receptors. Nature. 1972 Apr 21;236(5347):385–390. doi: 10.1038/236385a0. [DOI] [PubMed] [Google Scholar]
  5. Cabero J. L., Li Z. Q., Mårdh S. Gastrin potentiates histamine-stimulated aminopyrine accumulation in isolated rat parietal cells. Am J Physiol. 1991 Oct;261(4 Pt 1):G621–G627. doi: 10.1152/ajpgi.1991.261.4.G621. [DOI] [PubMed] [Google Scholar]
  6. Chew C. S., Ljungström M., Smolka A., Brown M. R. Primary culture of secretagogue-responsive parietal cells from rabbit gastric mucosa. Am J Physiol. 1989 Jan;256(1 Pt 1):G254–G263. doi: 10.1152/ajpgi.1989.256.1.G254. [DOI] [PubMed] [Google Scholar]
  7. Code C. F., Maslinski S. M., Mossini F., Navert H. Methyl histamines and gastric secretion. J Physiol. 1971 Sep;217(3):557–571. doi: 10.1113/jphysiol.1971.sp009586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Coruzzi G., Bertaccini G., Schwartz J. C. Evidence that histamine H3 receptors are involved in the control of gastric acid secretion in the conscious cat. Naunyn Schmiedebergs Arch Pharmacol. 1991 Feb;343(2):225–227. doi: 10.1007/BF00168615. [DOI] [PubMed] [Google Scholar]
  9. Kondo S., Shinomura Y., Kanayama S., Kawabata S., Miyazaki Y., Imamura I., Fukui H., Matsuzawa Y. Interleukin-1 beta inhibits gastric histamine secretion and synthesis in the rat. Am J Physiol. 1994 Dec;267(6 Pt 1):G966–G971. doi: 10.1152/ajpgi.1994.267.6.G966. [DOI] [PubMed] [Google Scholar]
  10. Kopin A. S., Lee Y. M., McBride E. W., Miller L. J., Lu M., Lin H. Y., Kolakowski L. F., Jr, Beinborn M. Expression cloning and characterization of the canine parietal cell gastrin receptor. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3605–3609. doi: 10.1073/pnas.89.8.3605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Korte A., Myers J., Shih N. Y., Egan R. W., Clark M. A. Characterization and tissue distribution of H3 histamine receptors in guinea pigs by N alpha-methylhistamine. Biochem Biophys Res Commun. 1990 May 16;168(3):979–986. doi: 10.1016/0006-291x(90)91125-c. [DOI] [PubMed] [Google Scholar]
  12. LIN T. M., ALPHIN R. S., HENDERSON F. G., BENSLAY D. N., CHEN K. K. The role of histamine in gastric hydrochloric acid secretion. Ann N Y Acad Sci. 1962 Feb 28;99:30–44. doi: 10.1111/j.1749-6632.1962.tb45284.x. [DOI] [PubMed] [Google Scholar]
  13. Lam S. K., Koo J. Gastrin sensitivity in duodenal ulcer. Gut. 1985 May;26(5):485–490. doi: 10.1136/gut.26.5.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lutton D. A., Bamford K. B., O'Loughlin B., Ennis M. Modulatory action of Helicobacter pylori on histamine release from mast cells and basophils in vitro. J Med Microbiol. 1995 Jun;42(6):386–393. doi: 10.1099/00222615-42-6-386. [DOI] [PubMed] [Google Scholar]
  15. Marshall B. J., Armstrong J. A., McGechie D. B., Glancy R. J. Attempt to fulfil Koch's postulates for pyloric Campylobacter. Med J Aust. 1985 Apr 15;142(8):436–439. doi: 10.5694/j.1326-5377.1985.tb113443.x. [DOI] [PubMed] [Google Scholar]
  16. Moss S. F., Calam J. Acid secretion and sensitivity to gastrin in patients with duodenal ulcer: effect of eradication of Helicobacter pylori. Gut. 1993 Jul;34(7):888–892. doi: 10.1136/gut.34.7.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Prinz C., Kajimura M., Scott D. R., Mercier F., Helander H. F., Sachs G. Histamine secretion from rat enterochromaffinlike cells. Gastroenterology. 1993 Aug;105(2):449–461. doi: 10.1016/0016-5085(93)90719-s. [DOI] [PubMed] [Google Scholar]
  18. Queiroz D. M., Mendes E. N., Rocha G. A., Barbosa A. J., Carvalho A. S., Cunha-Melo J. R. Histamine concentration of gastric mucosa in Helicobacter pylori positive and negative children. Gut. 1991 May;32(5):464–466. doi: 10.1136/gut.32.5.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Queiroz D. M., Mendes E. N., Rocha G. A., Cunha-Melo J. R., Barbosa A. J., Lima G. F., Jr, Oliveira C. A. Helicobacter pylori and gastric histamine concentrations. J Clin Pathol. 1991 Jul;44(7):612–613. doi: 10.1136/jcp.44.7.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Queiroz D. M., Mendes E. N., Rocha G. A., Moura S. B., Resende L. M., Cunha-Melo J. R., Coelho L. G., Passos M. C., Oliveira C. A., Lima Júnior G. F. Histamine content of the oxyntic mucosa from duodenal ulcer patients: effect of Helicobacter pylori eradication. Am J Gastroenterol. 1993 Aug;88(8):1228–1232. [PubMed] [Google Scholar]
  21. Sobala G. M., Crabtree J. E., Dixon M. F., Schorah C. J., Taylor J. D., Rathbone B. J., Heatley R. V., Axon A. T. Acute Helicobacter pylori infection: clinical features, local and systemic immune response, gastric mucosal histology, and gastric juice ascorbic acid concentrations. Gut. 1991 Nov;32(11):1415–1418. doi: 10.1136/gut.32.11.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Soldani G., Intorre L., Bertini S., Luchetti E., Coruzzi G., Bertaccini G. Regulation of gastric acid secretion by histamine H3 receptors in the dog: an investigation into the site of action. Naunyn Schmiedebergs Arch Pharmacol. 1994 Aug;350(2):218–223. doi: 10.1007/BF00241100. [DOI] [PubMed] [Google Scholar]
  23. Soldani G., Mengozzi G., Intorre L., De Giorgi G., Coruzzi G., Bertaccini G. Histamine H3 receptor-mediated inhibition of gastric acid secretion in conscious dogs. Naunyn Schmiedebergs Arch Pharmacol. 1993 Jan;347(1):61–65. doi: 10.1007/BF00168773. [DOI] [PubMed] [Google Scholar]
  24. Soll A. H. Potentiating interactions of gastric stimulants on [14 C] aminopyrine accumulation by isolated canine parietal cells. Gastroenterology. 1982 Jul;83(1 Pt 2):216–223. [PubMed] [Google Scholar]
  25. Soll A. H. Secretagogue stimulation of [14C]aminopyrine accumulation by isolated canine parietal cells. Am J Physiol. 1980 Apr;238(4):G366–G375. doi: 10.1152/ajpgi.1980.238.4.G366. [DOI] [PubMed] [Google Scholar]
  26. Soll A. H. The actions of secretagogues on oxygen uptake by isolated mammalian parietal cells. J Clin Invest. 1978 Feb;61(2):370–380. doi: 10.1172/JCI108947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vuyyuru L., Schubert M. L., Harrington L., Arimura A., Makhlouf G. M. Dual inhibitory pathways link antral somatostatin and histamine secretion in human, dog, and rat stomach. Gastroenterology. 1995 Nov;109(5):1566–1574. doi: 10.1016/0016-5085(95)90645-2. [DOI] [PubMed] [Google Scholar]
  28. Wang L., Lucey M. R., Fras A. M., Wilson E. J., Del Valle J. Epidermal growth factor and transforming growth factor-alpha directly inhibit parietal cell function through a similar mechanism. J Pharmacol Exp Ther. 1993 Apr;265(1):308–313. [PubMed] [Google Scholar]
  29. Warren G. Protein transport. Signals and salvage sequences. Nature. 1987 May 7;327(6117):17–18. doi: 10.1038/327017a0. [DOI] [PubMed] [Google Scholar]
  30. West R. E., Jr, Zweig A., Shih N. Y., Siegel M. I., Egan R. W., Clark M. A. Identification of two H3-histamine receptor subtypes. Mol Pharmacol. 1990 Nov;38(5):610–613. [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES