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. 1983 Jun;46(6):500–504. doi: 10.1136/jnnp.46.6.500

An evaluation of cerebrospinal fluid oligoclonal banding confirmed by immunofixation on agarose gel.

P M George, M A Lorier, I M Donaldson
PMCID: PMC1027438  PMID: 6875583

Abstract

The cerebrospinal fluid (CSF) from 115 consecutive patients undergoing diagnostic lumbar puncture or myelography was examined to determine the usefulness of immunofixation, following agarose gel electrophoresis, in the detection of oligoclonal IgG. All electrophoretic patterns were evaluated with and without immunofixation, and the interpretation of 9% of specimens was altered by immunofixation. The demonstration of oligoclonal IgG was shown to be more reliable in the diagnosis of multiple sclerosis than other indices of intrathecal synthesis of IgG. It is concluded that immunofixation should be used routinely when examining CSF for oligoclonal banding.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloomer L. C., Bray P. F. Relative value of three laboratory methods in the diagnosis of multiple sclerosis. Clin Chem. 1981 Dec;27(12):2011–2013. [PubMed] [Google Scholar]
  2. Gerson B., Cohen S. R., Gerson I. M., Guest G. H. Myelin basic protein, oligoclonal bands, and IgG in cerebrospinal fluid as indicators of multiple sclerosis. Clin Chem. 1981 Dec;27(12):1974–1977. [PubMed] [Google Scholar]
  3. Gerson B., Krolikowski F. J., Gerson I. M. Two agarose electrophoretic systems for demonstration of oligoclonal bands in cerebrospinal fluid compared. Clin Chem. 1980 Feb;26(2):343–345. [PubMed] [Google Scholar]
  4. Gerson B., Orr J. M. Oligoclonal bands and quantitation of IgG in cerebrospinal fluid as indicators of multiple sclerosis. Am J Clin Pathol. 1980 Jan;73(1):87–91. doi: 10.1093/ajcp/73.1.87. [DOI] [PubMed] [Google Scholar]
  5. Jeppsson J. O., Laurell C. B., Franzén B. Agarose gel electrophoresis. Clin Chem. 1979 Apr;25(4):629–638. [PubMed] [Google Scholar]
  6. Johnson K. P. Cerebrospinal fluid and blood assays of diagnostic usefulness in multiple sclerosis. Neurology. 1980 Jul;30(7 Pt 2):106–109. doi: 10.1212/wnl.30.7_part_2.106. [DOI] [PubMed] [Google Scholar]
  7. Keshgegian A. A., Coblentz J., Lisak R. P. (University of Pennsylvania Case Conference): oligoclonal immunoglobulins in cerebrospinal fluid in multiple sclerosis. Clin Chem. 1980 Aug;26(9):1340–1345. [PubMed] [Google Scholar]
  8. Laurell C. B. Electroimmuno assay. Scand J Clin Lab Invest Suppl. 1972;124:21–37. doi: 10.3109/00365517209102748. [DOI] [PubMed] [Google Scholar]
  9. Laurenzi M. A., Link H. Comparison between agarose gel electrophoresis and isoelectric focusing of CSF for demonstration of oligoclonal immunoglobulin bands in neurological disorders. Acta Neurol Scand. 1978 Sep;58(3):148–156. doi: 10.1111/j.1600-0404.1978.tb02874.x. [DOI] [PubMed] [Google Scholar]
  10. Link H. Comparison of electrophoresis on agar gel and agarose gel in the evaluation of gamma-globulin abnormalities in cerebrospinal fluid and serum in multiple sclerosis. Clin Chim Acta. 1973 Jul 27;46(4):383–390. doi: 10.1016/0009-8981(73)90251-9. [DOI] [PubMed] [Google Scholar]
  11. Link H., Laurenzi M. A. Immunoglobulin class and light chain type of oligoclonal bands in CSF in multiple sclerosis determined by agarose gel electrophoresis and immunofixation. Ann Neurol. 1979 Aug;6(2):107–110. doi: 10.1002/ana.410060205. [DOI] [PubMed] [Google Scholar]
  12. Link H., Tibbling G. Principles of albumin and IgG analyses in neurological disorders. II. Relation of the concentration of the proteins in serum and cerebrospinal fluid. Scand J Clin Lab Invest. 1977 Sep;37(5):391–396. doi: 10.1080/00365517709091497. [DOI] [PubMed] [Google Scholar]
  13. Link H., Tibbling G. Principles of albumin and IgG analyses in neurological disorders. III. Evaluation of IgG synthesis within the central nervous system in multiple sclerosis. Scand J Clin Lab Invest. 1977 Sep;37(5):397–401. doi: 10.1080/00365517709091498. [DOI] [PubMed] [Google Scholar]
  14. MCALPINE D. The benign form of multiple sclerosis. A study based on 241 cases seen within three years of onset and followed up until the tenth year or more of the disease. Brain. 1961 Jun;84:186–203. doi: 10.1093/brain/84.2.186. [DOI] [PubMed] [Google Scholar]
  15. McDonald W. I., Halliday A. M. Diagnosis and classification of multiple sclerosis. Br Med Bull. 1977 Jan;33(1):4–9. [PubMed] [Google Scholar]
  16. Ritchie R. F., Smith R. Immunofixation. I. General principles and application to agarose gel electrophoresis. Clin Chem. 1976 Apr;22(4):497–499. [PubMed] [Google Scholar]
  17. Sidén A., Kjellin K. G. CSF protein examinations with thin-layer isoelectric focusing in multiple sclerosis. J Neurol Sci. 1978 Nov;39(1):131–146. doi: 10.1016/0022-510x(78)90194-6. [DOI] [PubMed] [Google Scholar]
  18. Sidén A., Kjellin K. G. Isoelectric focusing of CSF proteins in known or probable infectious neurological diseases and the Guillain-Barré syndrome. J Neurol Sci. 1979 Jun;42(1):139–153. doi: 10.1016/0022-510x(79)90158-8. [DOI] [PubMed] [Google Scholar]
  19. Strony L. P., Wagner K., Keshgegian A. A. Demonstration of cerebrospinal fluid oligoclonal banding in neurologic diseases by agarose gel electrophoresis and immunofixation. Clin Chim Acta. 1982 Jul 1;122(2):203–212. doi: 10.1016/0009-8981(82)90279-0. [DOI] [PubMed] [Google Scholar]
  20. Thompson E. J., Kaufmann P., Shortman R. C., Rudge P., McDonald W. I. Oligoclonal immunoglobulins and plasma cells in spinal fluid of patients with multiple sclerosis. Br Med J. 1979 Jan 6;1(6155):16–17. doi: 10.1136/bmj.1.6155.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tibbling G., Link H., Ohman S. Principles of albumin and IgG analyses in neurological disorders. I. Establishment of reference values. Scand J Clin Lab Invest. 1977 Sep;37(5):385–390. doi: 10.1080/00365517709091496. [DOI] [PubMed] [Google Scholar]
  22. Tourtellotte W. On cerebrospinal fluid immunoglobulin-G (IgG) quotients in multiple sclerosis and other diseases. A review and a new formula to estimate the amount of IgG synthesized per day by the central nervous system. J Neurol Sci. 1970 Mar;10(3):279–304. doi: 10.1016/0022-510x(70)90156-5. [DOI] [PubMed] [Google Scholar]

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