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. 2023 Jun 17;17(5):220541. doi: 10.20964/2022.05.13

Electrochemical biosensors for the detection of SARS-CoV-2 pathogen and protein biomarkers

Yintang Zhang 1,2, Fang Chen 1,2, Hao Xie 3, Binbin Zhou 3
PMCID: PMC10276346  PMID: 37360860

Abstract

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV CoV-2) pathogen and protein biomarkers can improve the diagnosis accuracy for Coronavirus disease 2019 (COVID-19). Electrochemical biosensors have attracted extensive attention in the scientific community because of their simple design, fast response, good portability, high sensitivity and high selectivity. In this review, we summarized the progress in the electrochemical detection of COVID-19 pathogen and SARS-CoV-2 biomarkers, including SARS-CoV-2 spike protein and nucleocapsid protein and their antibodies.

Keywords: Coronavirus disease 2019, electrochemical biosensors, SARS-CoV-2 spike protein, SARS-CoV-2 nucleocapsid protein, antibody

References

  • 1.Chauhan D.S., Prasad R., Srivastava R., Jaggi M., Chauhan S.C., Yallapu M.M. Bioconjug. Chem. 2020;31:2021. doi: 10.1021/acs.bioconjchem.0c00323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Song Q., Sun X., Dai Z., Gao Y., Gong X., Zhou B., Wu J., Wen W. Lab Chip. 2021;21:1634. doi: 10.1039/d0lc01156h. [DOI] [PubMed] [Google Scholar]
  • 3.Xi H., Jiang H., Juhas M., Zhang Y. ACS Omega. 2021;6:25846. doi: 10.1021/acsomega.1c04024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Xi H., Juhas M., Zhang Y. Biosens. Bioelectron. 2020;167 doi: 10.1016/j.bios.2020.112494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Aziz A., Asif M., Ashraf G., Farooq U., Yang Q., Wang S. Curr. Opin. Colloid Interface Sci. 2021;52 doi: 10.1016/j.cocis.2021.101418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Garg M., Sharma A.L., Singh S. Biosens. Bioelectron. 2021;171 doi: 10.1016/j.bios.2020.112703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Cui F., Zhou H.S. Biosens. Bioelectron. 2020;165 doi: 10.1016/j.bios.2020.112349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Lim W.Y., Lan B.L., Ramakrishnan N. Biosensors. 2021;11:434. doi: 10.3390/bios11110434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Parihar A., Ranjan P., Sanghi S.K., Srivastava A.K., Khan R. ACS Appl. Bio. Mater. 2020;3:7326. doi: 10.1021/acsabm.0c01083. [DOI] [PubMed] [Google Scholar]
  • 10.Chang Y., Ma X., Sun T., Liu L., Hao Y. Talanta. 2021;234 doi: 10.1016/j.talanta.2021.122649. [DOI] [PubMed] [Google Scholar]
  • 11.Xia N., Sun T., Liu L., Tia L., Sun Z. Talanta. 2022;237 doi: 10.1016/j.talanta.2021.122949. [DOI] [PubMed] [Google Scholar]
  • 12.Imran S., Ahmadi S., Kerman K. Micromachines. 2021;12:174. doi: 10.3390/mi12020174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Liu L., Deng D., Wu D., Hou W., Wang L., Li N., Sun Z. Anal. Chim. Acta. 2021;1149 doi: 10.1016/j.aca.2021.338199. [DOI] [PubMed] [Google Scholar]
  • 14.Sun T., Guo Y., Zhao F. Int. J. Electrochem. Sci. 2021;16 [Google Scholar]
  • 15.Kaushik A.K., Dhau J.S., Gohel H., Mishra Y.K., Kateb B., Kim N.Y., Goswami D.Y. ACS Appl. Bio. Mater. 2020;3:7306. doi: 10.1021/acsabm.0c01004. [DOI] [PubMed] [Google Scholar]
  • 16.Ranjan P., Singhal A., Yadav S., Kumar N., Murali S., Sanghi S.K., Khan R. Int. Rev. Immunol. 2021;40:126. doi: 10.1080/08830185.2021.1872566. [DOI] [PubMed] [Google Scholar]
  • 17.Kudr J., Michalek P., Ilieva L., Adam V., Zitka O. TrAC-Trend. Anal. Chem. 2021;136 doi: 10.1016/j.trac.2021.116192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Kumar N., Shetti N.P., Jagannath S., Aminabhavi T.M. Chem. Eng. J. 2022;430 doi: 10.1016/j.cej.2021.132966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Mahshid S.S., Flynn S.E., Mahshid S. Biosens. Bioelectron. 2021;176 doi: 10.1016/j.bios.2020.112905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Campos-Ferreira D., Visani V., Cordula C., Nascimento G.A., Montenegro L. M.L., Schindler H.C., Cavalcanti I. M.F. Biochem. Eng. J. 2021;176 doi: 10.1016/j.bej.2021.108200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Jiang C., Mu X., Du B., Tong Z. Micro Nano Lett. 2021 doi: 10.1049/mna2.12101. [DOI] [Google Scholar]
  • 22.Eissa S., Zourob M. Anal. Chem. 2021;93:1826. doi: 10.1021/acs.analchem.0c04719. [DOI] [PubMed] [Google Scholar]
  • 23.Vezza V.J., Butterworth A., Lasserre P., Blair E.O., MacDonald A., Hannah S., Rinaldi C., Hoskisson P.A., Ward A.C., Longmuir A., Setford S., Farmer E. C.W., Murphy M.E., Corrigan D.K. Chem. Commun. 2021;57:3704. doi: 10.1039/d1cc00936b. [DOI] [PubMed] [Google Scholar]
  • 24.de Lima L.F., Ferreira A.L., Torres M. D.T., de Araujo W.R., de la Fuente-Nunez C. Proc. Natl. Acad. Sci. USA. 2021;118 doi: 10.1073/pnas.2106724118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Fabiani L., Saroglia M., Galata G., De Santis R., Fillo S., Luca V., Faggioni G., D'Amore N., Regalbuto E., Salvatori P., Terova G., Moscone D., Lista F., Arduini F. Biosens. Bioelectron. 2021;171 doi: 10.1016/j.bios.2020.112686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Stefano J.S., Guterres E. S. L.R., Rocha R.G., Brazaca L.C., Richter E.M., Abarza Munoz R.A., Janegitz B.C. Anal. Chim. Acta. 2022;1191 doi: 10.1016/j.aca.2021.339372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Yousefi H., Mahmud A., Chang D., Das J., Gomis S., Chen J.B., Wang H., Been T., Yip L., Coomes E., Li Z., Mubareka S., McGeer A., Christie N., Gray-Owen S., Cochrane A., Rini J.M., Sargent E.H., Kelley S.O. J. Am. Chem. Soc. 2021;143:1722. doi: 10.1021/jacs.0c10810. [DOI] [PubMed] [Google Scholar]
  • 28.Li J., Lin R., Yang Y., Zhao R., Song S., Zhou Y., Shi J., Wang L., Song H., Hao R. ACS Appl. Mater. Interfaces. 2021;13:22262. doi: 10.1021/acsami.1c05770. [DOI] [PubMed] [Google Scholar]
  • 29.Rashed M.Z., Kopechek J.A., Priddy M.C., Hamorsky K.T., Palmer K.E., Mittal N., Valdez J., Flynn J., Williams S.J. Biosens. Bioelectron. 2021;171 doi: 10.1016/j.bios.2020.112709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Nandeshwar R., Kumar M.S., Kondabagil K., Tallur S. IEEE Access. 2021;9 [Google Scholar]
  • 31.Liv L., Yener M., Coban G., Can S.A. Anal. Bioanal. Chem. 2022;414:1313. doi: 10.1007/s00216-021-03752-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Tabrizi M.A., Fernandez-Blazquez J.P., Medina D.M., Acedo P. Biosens. Bioelectron. 2022;196 doi: 10.1016/j.bios.2021.113729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Raziq A., Kidakova A., Boroznjak R., Reut J., Opik A., Syritski V. Biosens. Bioelectron. 2021;178 doi: 10.1016/j.bios.2021.113029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Zhang T., Sun L., Zhang Y. Anal. Methods. 2021;13:5772. doi: 10.1039/d1ay01478a. [DOI] [PubMed] [Google Scholar]
  • 35.Torres M. D.T., de Araujo W.R., de Lima L.F., Ferreira A.L., de la Fuente-Nunez C. Matter. 2021;4:2403–2416. doi: 10.1016/j.matt.2021.05.003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Ali M.A., Hu C., Jahan S., Yuan B., Saleh M.S., Ju E., Gao S.J., Panat R. Adv. Mater. 2021;33 doi: 10.1002/adma.202006647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Rahmati Z., Roushani M., Hosseini H., Choobin H. Microchim. Acta. 2021;188:105. doi: 10.1007/s00604-021-04762-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Yakoh A., Pimpitak U., Rengpipat S., Hirankarn N., Chailapakul O., Chaiyo S. Biosens. Bioelectron. 2021;176 doi: 10.1016/j.bios.2020.112912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 39.Eissa S., Alhadrami H.A., Al-Mozaini M., Hassan A.M., Zourob M. Microchim. Acta. 2021;188:199. doi: 10.1007/s00604-021-04867-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Liv L., Coban G., Nakiboglu N., Kocagoz T. Biosens. Bioelectron. 2021;192 doi: 10.1016/j.bios.2021.113497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Tan D., Li F., Zhou B. Int. J. Electrochem. Sci. 2020;15:9446. [Google Scholar]
  • 42.Singhal A., Parihar A., Kumar N., Khan R. Mater. Lett. 2022;306 doi: 10.1016/j.matlet.2021.130898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Tran V.V., Tran N. H.T., Hwang H.S., Chang M. Biosens. Bioelectron. 2021;182 doi: 10.1016/j.bios.2021.113192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 44.Bisht A., Mishra A., Bisht H., Tripathi R.M. J. Anal. Test. 2021;5:327. doi: 10.1007/s41664-021-00200-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Balkourani G., Brouzgou A., Archonti M., Papandrianos N., Song S., Tsiakaras P. J. Electroanal. Chem. 2021;893 doi: 10.1016/j.jelechem.2021.115289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 46.Gowri A., Ashwin Kumar N., Suresh Anand B.S. TrAC-Trend. Anal. Chem. 2021;137 doi: 10.1016/j.trac.2021.116205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 47.Xia N., Wang X., Yu J., Wu Y., Cheng S., Xing Y., Liu L. Sens. Actuat. B: Chem. 2017;239:834. [Google Scholar]
  • 48.Deng D., Hao Y., Yang S., Han Q., Liu L., Xiang Y., Tu F., Xia N. Sens. Actuat. B: Chem. 2019;286:415. [Google Scholar]
  • 49.Rahmati Z., Roushani M., Hosseini H., Choobin H. Microchem. J. 2021;170 doi: 10.1016/j.microc.2021.106718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 50.Torrente-Rodríguez R.M., Lukas H., Tu J., Min J., Yang Y., Xu C., Rossiter H.B., Gao W. Matter. 2020;3:1981. doi: 10.1016/j.matt.2020.09.027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 51.Zhou L., Hao P., Li H., Zhang Z. ACS Sens. 2021;6:1613. doi: 10.1021/acssensors.1c00022. [DOI] [PubMed] [Google Scholar]
  • 52.Karaman C., Yola B.B., Karaman O., Atar N., Polat I., Yola M.L. Microchim. Acta. 2021;188:425. doi: 10.1007/s00604-021-05092-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 53.Li J., Lillehoj P.B. ACS Sens. 2021;6:1270. doi: 10.1021/acssensors.0c02561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 54.Peng R., Pan Y., Li Z., Qin Z., Rini J.M., Liu X. Biosens. Bioelectron. 2022;197 doi: 10.1016/j.bios.2021.113762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 55.Chiang S.H., Tu M., Cheng J., Wei F., Li F., Chia D., Garner O., Chandrasekaran S., Bender R., Strom C.M., Wong D. T.W. PLoS One. 2021;16 doi: 10.1371/journal.pone.0251342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 56.Idili A., Parolo C., Alvarez-Diduk R., Merkoci A. ACS Sens. 2021;6:3093. doi: 10.1021/acssensors.1c01222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 57.Zhang Z., Pandey R., Li J., Gu J., White D., Stacey H.D., Ang J.C., Steinberg C.J., Capretta A., Filipe C. D.M., Mossman K., Balion C., Miller M.S., Salena B.J., Yamamura D., Soleymani L., Brennan J.D., Li Y. Angew. Chem. Int. Ed. 2021;60:24266. doi: 10.1002/anie.202110819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 58.Abrego-Martinez J.C., Jafari M., Chergui S., Pavel C., Che D., Siaj M. Biosens. Bioelectron. 2022;195 doi: 10.1016/j.bios.2021.113595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 59.Ramanathan S., Gopinath S. C.B., Ismail Z.H., Md Arshad M.K., Poopalan P. Biosens. Bioelectron. 2022;197 doi: 10.1016/j.bios.2021.113735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 60.Zhai Q., Yap L.W., Wang R., Gong S., Guo Z., Liu Y., Lyu Q., Wang J., Simon G.P., Cheng W. Anal. Chem. 2020;92:4647. doi: 10.1021/acs.analchem.0c00274. [DOI] [PubMed] [Google Scholar]

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