Table 1. Primers and their sequences used in this study.
Genes | Primer sequences | Product (bp) | Annealing temperature (°C) |
mcr-1 | F: CACTTATGGCACGGTCTATGA | 956 bp | 59 |
R: CCCAAACCAATGATACGCAT | |||
mcr-2 | F: TGGTACAGCCCCTTTATT | 1617 bp | 57 |
R: GCTTGAGATTGGGTTATGA | |||
mcr-3 | F: TTGGCACTGTATTTTGCATTT | 542 bp | 50 |
R: TAACGAAATTGGCTGGAACA |