FIG 5.

Increased sterol content in the absence of ORP1 in macrophages. (A) Representative images of wild-type and ORP1-null MH-S macrophages infected with mCherry-expressing C. burnetii for 6 days and stained with filipin to visualize cholesterol. Filipin intensity scale images show strong filipin signal on the CCV in ORP1-null macrophages. White arrows indicate CCVs. Scale bars, 10 μm. (B) Filipin intensity in CCVs of WT and ORP1-null macrophages, where intensity values were normalized to area and background intensity was subtracted from CCV intensity. Data shown are means ± SEM of at least 20 cells per condition in each of three independent experiments as determined by a nonparametric Mann-Whitney test. ****, P < 0.0001.