Skip to main content
NCBI home page
Archives of Disease in Childhood logoLink to Archives of Disease in Childhood
. 1993 Feb;68(2):198–201. doi: 10.1136/adc.68.2.198

Poliovirus antibody titres, relative affinity, and neutralising capacity in maternal milk.

S Zaman 1, B Carlsson 1, A Morikawa 1, S Jeansson 1, I Narayanan 1, K Thiringer 1, F Jalil 1, L A Hanson 1
PMCID: PMC1029234  PMID: 8386918

Abstract

Varying titres of secretory IgA antibodies to poliovirus type 1 were found previously in the milk of unvaccinated, lactating Pakistani mothers during two different years, reflecting the antigenic exposure on mucosal membranes. To study further the changes in the extent and the form of antigenic exposure reflected in the human milk, human milk samples from Pakistani, Indian, Japanese, and Swedish mothers were collected. The quality and the neutralising capacity of the antibodies was also studied. Secretory IgA, IgG, and IgM antibodies to poliovirus type 1 were determined using enzyme linked immunosorbent assay (ELISA) and relative affinity was measured in ELISA by elution with potassium thiocyanide. Microneutralisation tests were also performed. The higher secretory IgA antibody titres to poliovirus type 1 in the unvaccinated, naturally exposed Pakistani and Indian mothers' milk, compared with the Swedish and Japanese mothers, presumably reflect the epidemiological situation in these countries. Neutralising capacity and the relative antibody affinity seemed to be higher both in the Pakistani mothers and the group without natural exposure but only given inactivated poliovirus vaccine, that is the Swedish mothers, than the group meeting only live vaccine strains, that is the Japanese mothers.

Full text

PDF
198

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlsson B., Ahlstedt S., Hanson L. A., Lidin-Janson G., Lindblad B. S., Sultana R. Escherichia coli O antibody content in milk from healthy Swedish mothers and mothers from a very low socio-economic group of a developing country. Acta Paediatr Scand. 1976 Jul;65(4):417–423. doi: 10.1111/j.1651-2227.1976.tb04908.x. [DOI] [PubMed] [Google Scholar]
  2. Carlsson B., Zaman S., Mellander L., Jalil F., Hanson L. A. Secretory and serum immunoglobulin class-specific antibodies to poliovirus after vaccination. J Infect Dis. 1985 Dec;152(6):1238–1244. doi: 10.1093/infdis/152.6.1238. [DOI] [PubMed] [Google Scholar]
  3. Cruz J. R., Carlsson B. V., Hofvander Y., Holme D. T., Hanson L. A. Studies of human milk. II. Concentration of antibodies against Salmonella and Shigella in milk of women from different populations and the daily intake by their breast-fed infants. Acta Paediatr Scand. 1985 May;74(3):338–341. doi: 10.1111/j.1651-2227.1985.tb10980.x. [DOI] [PubMed] [Google Scholar]
  4. Cruz J. R., Carlsson B., García B., Gebre-Medhin M., Hofvander Y., Urrutia J. J., Hanson L. A. Studies on human milk III. Secretory IgA quantity and antibody levels against Escherichia coli in colostrum and milk from underprivileged and privileged mothers. Pediatr Res. 1982 Apr;16(4 Pt 1):272–276. doi: 10.1203/00006450-198204000-00004. [DOI] [PubMed] [Google Scholar]
  5. Czerkinsky C., Prince S. J., Michalek S. M., Jackson S., Russell M. W., Moldoveanu Z., McGhee J. R., Mestecky J. IgA antibody-producing cells in peripheral blood after antigen ingestion: evidence for a common mucosal immune system in humans. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2449–2453. doi: 10.1073/pnas.84.8.2449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dimmock N. J. Mechanisms of neutralization of animal viruses. J Gen Virol. 1984 Jun;65(Pt 6):1015–1022. doi: 10.1099/0022-1317-65-6-1015. [DOI] [PubMed] [Google Scholar]
  7. Glass R. I., Svennerholm A. M., Stoll B. J., Khan M. R., Hossain K. M., Huq M. I., Holmgren J. Protection against cholera in breast-fed children by antibodies in breast milk. N Engl J Med. 1983 Jun 9;308(23):1389–1392. doi: 10.1056/NEJM198306093082304. [DOI] [PubMed] [Google Scholar]
  8. Grillner L., Blomberg J. Hemolysis-in-gel and neutralization tests for determination of antibodies to mumps virus. J Clin Microbiol. 1976 Jul;4(1):11–15. doi: 10.1128/jcm.4.1.11-15.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haliburton G., Becker M. E. The use of HEPES buffer in micro-tissue culture plates for routine enterovirus diagnosis. Health Lab Sci. 1971 Jul;8(3):155–159. [PubMed] [Google Scholar]
  10. Hjelt K., Grauballe P. C., Nielsen O. H., Schiøtz P. O., Krasilnikoff P. A. Rotavirus antibodies in the mother and her breast-fed infant. J Pediatr Gastroenterol Nutr. 1985 Jun;4(3):414–420. doi: 10.1097/00005176-198506000-00016. [DOI] [PubMed] [Google Scholar]
  11. Isaacs C. E., Thormar H., Pessolano T. Membrane-disruptive effect of human milk: inactivation of enveloped viruses. J Infect Dis. 1986 Dec;154(6):966–971. doi: 10.1093/infdis/154.6.966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mestecky J. The common mucosal immune system and current strategies for induction of immune responses in external secretions. J Clin Immunol. 1987 Jul;7(4):265–276. doi: 10.1007/BF00915547. [DOI] [PubMed] [Google Scholar]
  13. Passwell J. H., Steward M. W., Soothill J. F. The effects of protein malnutrition on macrophage function and the amount and affinity of antibody response. Clin Exp Immunol. 1974 Jul;17(3):491–495. [PMC free article] [PubMed] [Google Scholar]
  14. Plotkin S. A., Katz M., Brown R. E., Pagano J. S. Oral poliovirus vaccination in newborn African infants. The inhibitory effect of breast feeding. Am J Dis Child. 1966 Jan;111(1):27–30. doi: 10.1001/archpedi.1966.02090040063004. [DOI] [PubMed] [Google Scholar]
  15. Roberton D. M., Carlsson B., Coffman K., Hahn-Zoric M., Jalil F., Jones C., Hanson L. A. Avidity of IgA antibody to Escherichia coli polysaccharide and diphtheria toxin in breast milk from Swedish and Pakistani mothers. Scand J Immunol. 1988 Dec;28(6):783–789. doi: 10.1111/j.1365-3083.1988.tb01512.x. [DOI] [PubMed] [Google Scholar]
  16. Snodgrass D. R., Fahey K. J., Wells P. W., Campbell I., Whitelaw A. Passive immunity in calf rotavirus infections: maternal vaccination increases and prolongs immunoglobulin G1 antibody secretion in milk. Infect Immun. 1980 May;28(2):344–349. doi: 10.1128/iai.28.2.344-349.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Steward M. W., Petty R. E. Evidence for the genetic control of antibody affinity from breeding studies with inbred mouse strains producing high and low affinity antibody. Immunology. 1976 Jun;30(6):789–797. [PMC free article] [PubMed] [Google Scholar]
  18. Svennerholm A. M., Hanson L. A., Holmgren J., Jalil F., Lindblad B. S., Khan S. R., Nilsson A., Svennerholm B. Antibody responses to live and killed poliovirus vaccines in the milk of Pakistani and Swedish women. J Infect Dis. 1981 May;143(5):707–711. doi: 10.1093/infdis/143.5.707. [DOI] [PubMed] [Google Scholar]
  19. Svennerholm A. M., Hanson L. A., Holmgren J., Lindblad B. S., Nilsson B., Quereshi F. Different secretory immunoglobulin A antibody responses to cholera vaccination in Swedish and Pakistani women. Infect Immun. 1980 Nov;30(2):427–430. doi: 10.1128/iai.30.2.427-430.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WARREN R. J., LEPOW M. L., BARTSCH G. E., ROBBINS F. C. THE RELATIONSHIP OF MATERNAL ANTIBODY, BREAST FEEDING, AND AGE TO THE SUSCEPTIBILITY OF NEWBORN INFANTS TO INFECTION WITH ATTENUATED POLIOVIRUSES. Pediatrics. 1964 Jul;34:4–13. [PubMed] [Google Scholar]
  21. Zaman S., Carlsson B., Jalil F., Jeansson S., Mellander L., Hanson L. A. Specific antibodies to poliovirus type I in breastmilk of unvaccinated mothers before and seven years after start of community-wide vaccination of their infants with live, oral poliovirus vaccine. Acta Paediatr Scand. 1991 Dec;80(12):1174–1182. doi: 10.1111/j.1651-2227.1991.tb11806.x. [DOI] [PubMed] [Google Scholar]

Articles from Archives of Disease in Childhood are provided here courtesy of BMJ Publishing Group

RESOURCES