Photomicrographs of a rat testis of group II, showing: (a) Irregular outer boundaries (I), fused tubules (F), widely separated seminiferous tubules (IT), and dilated congested blood vessels (arrowheads). H&E, ×200. (b) Loss of outer boundaries of the tubules (arrows). H&E, ×200. (c) The separation between the basal layer and other spermatogenic cells (*), basal germ cells with very dark nuclei (arrows). H&E, ×400. (d) Deeply inserted spermatozoa in between spermatogenic cells (Sz), desquamated spermatogenic cells in the lumen (Sc), fused outer boundaries of two tubules (F). H&E, ×400. (e) Vacuolated spermatogonia (bifid arrows) and vacuolated primary spermatocytes (curved arrows). H&E, ×1000. (f) Vacuolated interstitial tissue (V) containing Leydig cells (L) with perinuclear vacuolation (N) and highly acidophilic cytoplasm (C). H&E, ×1000.