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. Author manuscript; available in PMC: 2024 Apr 28.
Published in final edited form as: Science. 2023 Apr 27;380(6643):382–387. doi: 10.1126/science.add7328

Fig. 3. RAD51 is not required for fork reversal when CMG is disassembled from the stalled replication fork.

Fig. 3.

(A-B) Fork protection assays were completed in MCM2-AID2 degron cells after transfection with siRNAs. 2μM 5-ph-IAA was added to induce MCM2 degradation. (C-D) Immunoblots of MCM3-AID2 and MCM4-AID2 degron cells. (E-F) Fork protection assays in the MCM3-AID2 and MCM4-AID2 degron cells. (G) Immunoblot of GINS4 degron cells. (H) Fork protection assay in the GINS4-AID2 degron cells. (I) MCM7 integrated intensity in the nucleus of GINS4-AID2 degron cells was measured by immunofluorescence. All graphs are representative of at least three experiments. P values were calculated using a Kruskal-Wallis test. (J) Percentage of reversed replication forks in MCM2-AID2 cells transfected with the indicated siRNA and treated 72 hours later with DMSO or 2μM 5-ph-IAA together with 4mM HU, Mirin, and C5 for 5 hours. The number of replication intermediates analyzed for each condition is indicated in parentheses.