Table 2.

Summary of the findings and experimental interpretations of the five principal components determined using the principal component analysis technique.

Principal Component	Percentage of the Data Related to Component	Main Associated Components	Correlation to Clinical Signs of Disease?	Interpretation
1	26.5%	Th1-biased inflammation markers in the blood and spleen, varying considerably between time points Viral titres in blood and spleen, rapid increase with slow decline	No	Systemic disease, with rapid conventional host response
2	18.5%	Th1-biased inflammation markers in the brain, varying considerably between time points Viral titres in brain, rapid increase over time	Yes	A strong association with clinical signs of disease (typically neurological), likely representative of the encephalitis typical of lethal infection
3	12.6%	Th2- and Th17-biased inflammation markers in all sample types did not differ between time points	No	Inter-experimental variation in flow cytometry analysis
4	7.0%	Cell counts in the blood and spleen did not vary between time points	No	Inter-experimental variation in luminex analysis
5	5.3%	Leukocyte counts in the blood, transient decline at the midway point of sampling	No	Leukopenia