Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| E. coli: XL1-Blue | QB3 Macrolab, UC Berkeley | N/A |
| E. coli: Rosetta2 (DE3) pLysS | QB3 Macrolab, UC Berkeley | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| DDM: n-Dodecyl-β-D-Maltoside | Anatrace | Cat# D310 |
| CHS: Cholesteryl Hemisuccinate Tris Salt | Anatrace | Cat# CH210 |
| Protein C peptide: EDQVDPRLIDGK | Genscript | N/A |
| POPC: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine | Avanti Polar Lipids | Cat# 850457 |
| POPG: 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (sodium salt) | Avanti Polar Lipids | Cat# 840457 |
| Bio-Beads SM-2 Adsorbents | BioRad | Cat# 1523920 |
| HisPur Ni-NTA Resin | Thermo Fisher Scientific | Cat# 88223 |
| Ibogaine hydrochloride | NIDA Drug Supply Program | NIDA # 7260-001 |
| Fluoxetine hydrochloride | Millipore Sigma | F132 |
| N,N-dimethylacetamide | Millipore Sigma | ARK-2190 |
| 2-Hydroxypropoyl-β-cyclodextrin | Millipore Sigma/TCI | 778966/H0979 |
| Sterile water | Corning (MediaTech) | 46-000-Cl |
| Sucrose (Quick Dissolve Superfine Pure Cane) | Domino | 411096 |
| Critical commercial assays | ||
| Q5 High-Fidelity 2X Master Mix | New England Bio-Labs | Cat# M0492 |
| NucleoBond Xtra Midi Plus kit | Macherey-Nagel | Cat# 740412.50 |
| ExpiFectamine 293 Transfection Kit | Thermo Fisher Scientific | Cat# A14524 |
| Deposited data | ||
| Coordinates: SERT-Fab15B8 ‘8090-bound | This paper | PDB: 7TXT |
| Experimental models: Cell lines | ||
| Human: Expi293F Inducible Cells | Thermo Fisher Scientific | Cat# A39241 |
| Experimental models: Organisms/strains | ||
| Vesicular monoamine transporter 2 mice | Wang et al., 1997 | N/A |
| C57BL/6J mice | The Jackson Laboratories | JAX: 000664 |
| Oligonucleotides | ||
| Primer: SERT Forward: CTGGGTACCAGCTGCTAGCAAGCTTGCTAGCgccacc ATGGAATTGCATCAGGGCGAACG | This paper | N/A |
| Primer: SERT Reverse: CTGATCAGCGGGTTTAAACGGGCCCTCTCTCCTATC ATTTCCCGTCTATCAACCTGG | This paper | N/A |
| Recombinant DNA | ||
| Plasmid for expression of SERT wild type | This paper | Addgene Plasmid #190743 |
| Plasmid for expression of SERT C109A_Y107C mutant | Jacobs et al., 2007 | Addgene Plasmid #190174 |
| Plasmid for expression of SERT X5C_S277C mutant | Zhang and Rudnick, 2006 | Addgene Plasmid #190177 |
| Fab15B8 Heavy chain | Coleman et al., 2019 | N/A |
| Fab15B8 Light chain | Coleman et al., 2019 | N/A |
| SERT | UniProt: P31645 | N/A |
| MSPNW11 | Nasr et al., 2017 | Laboratory of Gerhard Wagner |
| Plasmid: pcDNA3.1-zeocin-TetO | This paper | N/A |
| Plasmid: pcDNA3.4 | Thermo Fisher Scientific | Cat# A14697 |
| Software and algorithms | ||
| Serial EM | Mastronarde, 2003 | https://structura.bio/ |
| DOCK 3.7 | UCSF | https://dock.compbio.ucsf.edu/DOCK3.7/ |
| DOCK Blaster | UCSF | https://blaster.docking.org/ |
| ZINC20 | UCSF | https://zinc20.docking.org/ |
| CryoSparc v3.2.0 | Structura Biotechnology | https://structura.bio/ |
| Relion v.3.0 | Zivanov et al., 2018 | https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page |
| Pyem v0.5 | Asarnow et al., 2019 | https://github.com/asarnow/pyem |
| dFSC | Dang et al., 2017 | N/A |
| UCSF ChimeraX v1.4 | Goddard et al., 2018 | |
| ISOLDE | Croll, 2018 | https://isolde.cimr.cam.ac.uk/ |
| Phenix v1.20.1 | Adams et al., 2010 | http://www.phenix-online.org/ |
| SPSS Statistics 27 | IBM | https://www.ibm.com/products/spss-statistic |
| Prism 9.5.1 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| GraphPad Prism v9 | GraphPad Software | https://www.graphpad.com |
| Origin 2022b | OriginLab Corp. | https://www.originlab.com/ |
| Other | ||
| Q5 High-Fidelity 2X Master Mix | New England Bio-Labs | Cat# M0492 |
| NucleoBond Xtra Midi Plus kit | Macherey-Nagel | Cat# 740412.50 |
| ExpiFectamine 293 Transfection Kit | Thermo Fisher Scientific | Cat# A14524 |
| Holey Gold Supports: UltrAuFoil (R 1.2/1.3) Au300 mesh | Quantifoil Micro Tools | N1-A14nAu30-01 |
| Titan Krios Gi3 | Thermo Fisher Scientific | N/A |
| BioQuantum Energy Filter | Gatan | N/A |
| K3 Direct Electron Detector | Gatan | N/A |
| Mouse Tail Suspension apparatus | Med Associates | MED-TSS-MS |
| Shuttle Box apparatus | Med Associates | MED-APA-D1M |
| Pulse Ultra Bright LED Strobe Light with 108 LEDs | Roxant or Amazon.com | |
| Holder and Bottles for anhedonia testing | Animal Care Systems | C79125A |
| Open Field | Omnitech Electronics | VersaMax Legacy System |
| Fear-Potentiated Startle apparatus (shock threshold) | Med Associates | MED-ASR-PRO1 MED-ASR-FPS |