Figure 4. Cortical microvascular density in aged mice.

(a) Representative maximum intensity projection (MIP) images of the three-dimensional angiograms of three mice in the young sedentary group, three mice in the aged sedentary group, and three mice in the aged exercise group, over the cortical depth range from 50 to 400 μm, and 200 × 200 μm² field of view (FOV). Scale bars: 50 μm. (b) Vessel segment density and (c) vessel length density of cortical capillaries from young sedentary (n = 5 mice), aged sedentary (n = 9 mice), and aged exercise (n = 8 mice) groups. Student’s t-test. **p < 0.01; ***p < 0.001. Please see Supplementary document for exclusions.