In the original publication [1], there was a mistake in Figure 1 and Figure 2 as published.
In Figure 1, there is a loss of colour in most of the dots representing the farms on the map. The corrected Figure 1 appears below.
Figure 1.
A map of the lower region of Lake Volta in Ghana, West Africa, showing the date and location of the farms where the outbreaks of mortality occurred; locations retrieved from [11]. This map was constructed using ArcGIS (GIS software). Version 10.0. Redlands, CA, USA: Environmental Systems Research Institute, Inc., 2010.
In Figure 2, there is a loss of all dots. The corrected Figure 2 appears below.
Figure 2.
Successful recovery of >50% of the ISKNV genome required 482 template strands per µL (2410 viral templates per 5 µL sequencing reaction), with a minimum of 0.2 copies per µL to recover >0% of the genome with at least 20-fold coverage for error correction. Number of viral templates was measured using ddPCR from a serially diluted ISKNV template, which was subsequently sequenced and processed as described in the text.
The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Footnotes
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Reference
- 1.Alathari S., Chaput D.L., Bolaños L.M., Joseph A., Jackson V.L.N., Verner-Jeffreys D., Paley R., Tyler C.R., Temperton B. A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia. Viruses. 2023;15:965. doi: 10.3390/v15040965. [DOI] [PMC free article] [PubMed] [Google Scholar]