Extended Data Fig. 3. Selective increase of wild-type cell proliferation in the acute phase of injury-repair of mosaic skin.
a, b) Representative two-photon images of mitotic figures (white circles). c) Quantification of mitotic figures in tdTomato+ and tdTomato− areas in injured (I; n = 4 mice) and uninjured (U; n = 3 mice) wild-type and HrasG12V/+-mosaic. d) Representative two-photon images of apoptotic bodies (white circles) and quantification in tdTomato+ and tdTomato− areas in injured (I) and uninjured (U) wild-type and HrasG12V/+-mosaic. n = 4 mice. e) Representative two-photon images of the epidermal preparation immunofluorescence for active-Caspase-3 (white circle) and quantification of active-Caspase3+ cells in tdTomato+ and tdTomato− areas in injured (I) and uninjured (U) wild-type and HrasG12V/+-mosaic. n = 3 mice. f) Quantification of apoptotic bodies over time in tdTomato+ and tdTomato− areas in uninjured ears. Statistic between wild-type-mosaic and HrasG12V/+-mosaic in tdTomato− or tdTomato+ areas are represented with green or red dotted lines. g) Same quantification as in f) in injured ears. (f, g) n = 4 mice. h) β-galactosidase activity assay of epidermal preparations of uninjured and injured wild-type and HrasG12V/+-mosaic and positive controls: pancreas, kidney and provided by the kit. n = 3 mice for epidermal preparations. i) Quantification of cells expressing Keratin10 in tdTomato+ and tdTomato− areas in injured (I) wild-type and HrasG12V/+-mosaic. n = 3. Statistics: Paired, two-tailed t-test, comparing tdTomato+ and tdTomato− areas in the same group of mice. Unpaired, two-tailed t-test comparing tdTomato+ and tdTomato− populations in different groups of mice. Exact p-value reported on the figure. ns indicates not statistically significant. At least three independent areas of approximately 300 μm2 were analysed for each mouse (see Methods). Data are represented as means and standard deviations. Scale bar, 20 μm.