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. 2023 May 31;122(12):2605–2606. doi: 10.1016/j.bpj.2023.05.031

Measuring flow-mediated protein drift across stationary supported lipid bilayers

Amanda M Ratajczak, Sreeja Sasidharan, Xaymara I Rivera Gonzalez, Ethan J Miller, Larissa Socrier, Autumn A Anthony, Aurelia R Honerkamp-Smith
PMCID: PMC10323009  PMID: 37263274

Main text

(Biophysical Journal 122, 1720–1731; May 2, 2023)

In Figure 2F, the scale bar was incorrectly labeled as “100 μm”; it should read “20 μm“ instead. A corrected version of the figure appears below.

graphic file with name gr2c.jpg

Figure 2 (A and B) Rectangular microfluidic channels 300 μm in width and 100 μm in height were plasma-bonded to a glass coverslip. (C) Fluorescent membrane patches (red) overlaid with a bright-field image showing the edges of the microfluidic channel. (D–F) A single membrane patch labeled with both TXRed-DPPE (red, top row) and with fluorescent neutravidin (green, bottom row) shown before flow (left), during flow (middle), and 21 min after flow stopped (right). In all images, flow direction was from right to left. To see this figure in color, go online (corrected).

graphic file with name gr2o.jpg

Figure 2 (A and B) Rectangular microfluidic channels 300 μm in width and 100 μm in height were plasma-bonded to a glass coverslip. (C) Fluorescent membrane patches (red) overlaid with a bright-field image showing the edges of the microfluidic channel. (D–F) A single membrane patch labeled with both TXRed-DPPE (red, top row) and with fluorescent neutravidin (green, bottom row) shown before flow (left), during flow (middle), and 21 min after flow stopped (right). In all images, flow direction was from right to left. To see this figure in color, go online (original).


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