Figure 2. CG6178 shares 41% amino-acid identity with firefly luciferase.
Photinus pyralis firefly luciferase sequence (UniProt accession number: Q27758) aligned with the CG6178 sequence (UniProt accession number: Q9VCC6) using Clustal under MUSCLE settings.13 Amino acid residues within 5Å of the active site selected for mutagenesis are highlighted in yellow.