(A, C) Airyscan images of WT and GS28 KO cells stained for SNAP29, GM130 and STX5 show redistribution of SNAP29 to the Golgi region in GS28 KO cells. There is a significant increase in the colocalization between SNAP29 and GM130 in GS28 KO compared to WT. (B, C) Retro2-induced displacement of STX5 from the Golgi fails to recruit SNAP29 to the Golgi in GS28 KO cells. Airyscan images of WT and GS28 KO cells treated with Retro2 and stained for SNAP29, GM130 and STX5. Scale bars are 10μm, **p<0.001