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. 1987 Jan;50(1):57–65. doi: 10.1136/jnnp.50.1.57

Immunolocalisation of cytokeratins in the normal and neoplastic human pituitary gland.

J W Ironside, J A Royds, A A Jefferson, W R Timperley
PMCID: PMC1033250  PMID: 2434621

Abstract

Cytokeratins were studied by immunocytochemical techniques at light and electron microscopy on 12 normal pituitary glands, 30 pituitary adenomas and three craniopharyngiomas. The results are presented in relation to clinical and biochemical features and new information on the subcellular localisation of cytokeratins in pituitary cells is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asa S. L., Kovacs K. Functional morphology of the human fetal pituitary. Pathol Annu. 1984;19(Pt 1):275–315. [PubMed] [Google Scholar]
  2. Bendayan M., Roth J., Perrelet A., Orci L. Quantitative immunocytochemical localization of pancreatic secretory proteins in subcellular compartments of the rat acinar cell. J Histochem Cytochem. 1980 Feb;28(2):149–160. doi: 10.1177/28.2.7354212. [DOI] [PubMed] [Google Scholar]
  3. Berger G., Berger F., Bejui F., Bouvier R., Rochet M., Feroldi J. Bronchial carcinoid with fibrillary inclusions related to cytokeratins: an immunohistochemical and ultrastructural study with subsequent investigation of 12 foregut APUDomas. Histopathology. 1984 Mar;8(2):245–257. doi: 10.1111/j.1365-2559.1984.tb02339.x. [DOI] [PubMed] [Google Scholar]
  4. Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem. 1984 Jan;136(1):175–179. doi: 10.1016/0003-2697(84)90320-8. [DOI] [PubMed] [Google Scholar]
  5. Gabbiani G., Kapanci Y., Barazzone P., Franke W. W. Immunochemical identification of intermediate-sized filaments in human neoplastic cells. A diagnostic aid for the surgical pathologist. Am J Pathol. 1981 Sep;104(3):206–216. [PMC free article] [PubMed] [Google Scholar]
  6. Holthöfer H., Miettinen A., Paasivuo R., Lehto V. P., Linder E., Alfthan O., Virtanen I. Cellular origin and differentiation of renal carcinomas. A fluorescence microscopic study with kidney-specific antibodies, antiintermediate filament antibodies, and lectins. Lab Invest. 1983 Sep;49(3):317–326. [PubMed] [Google Scholar]
  7. Horvath E., Kovacs K. Morphogenesis and significance of fibrous bodies in human pituitary adenomas. Virchows Arch B Cell Pathol. 1978 Mar 2;27(1):69–78. doi: 10.1007/BF02888984. [DOI] [PubMed] [Google Scholar]
  8. Höfler H., Denk H., Walter G. F. Immunohistochemical demonstration of cytokeratins in endocrine cells of the human pituitary gland and in pituitary adenomas. Virchows Arch A Pathol Anat Histopathol. 1984;404(4):359–368. doi: 10.1007/BF00695220. [DOI] [PubMed] [Google Scholar]
  9. Ironside J. W., Royds J. A., Taylor C. B., Timperley W. R. Paraganglioma of the cauda equina: a histological, ultrastructural and immunocytochemical study of two cases with a review of the literature. J Pathol. 1985 Feb;145(2):195–201. doi: 10.1002/path.1711450207. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Makin C. A., Bobrow L. G., Bodmer W. F. Monoclonal antibody to cytokeratin for use in routine histopathology. J Clin Pathol. 1984 Sep;37(9):975–983. doi: 10.1136/jcp.37.9.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miettinen M., Lehto V. P., Virtanen I. Antibodies to intermediate filament proteins in the diagnosis and classification of human tumors. Ultrastruct Pathol. 1984;7(2-3):83–107. doi: 10.3109/01913128409141467. [DOI] [PubMed] [Google Scholar]
  13. Moll R., Franke W. W., Schiller D. L., Geiger B., Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11–24. doi: 10.1016/0092-8674(82)90400-7. [DOI] [PubMed] [Google Scholar]
  14. Neumann P. E., Goldman J. E., Horoupian D. S., Hess M. A. Fibrous bodies in growth hormone-secreting adenomas contain cytokeratin filaments. Arch Pathol Lab Med. 1985 Jun;109(6):505–508. [PubMed] [Google Scholar]
  15. Neumann P. E., Horoupian D. S., Goldman J. E., Hess M. A. Cytoplasmic filaments of Crooke's hyaline change belong to the cytokeratin class. An immunocytochemical and ultrastructural study. Am J Pathol. 1984 Aug;116(2):214–222. [PMC free article] [PubMed] [Google Scholar]
  16. Osborn M., Weber K. Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest. 1983 Apr;48(4):372–394. [PubMed] [Google Scholar]
  17. Ramaekers F., Huysmans A., Moesker O., Kant A., Jap P., Herman C., Vooijs P. Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology. Lab Invest. 1983 Sep;49(3):353–361. [PubMed] [Google Scholar]
  18. Schlegel R., Banks-Schlegel S., Pinkus G. S. Immunohistochemical localization of keratin in normal human tissues. Lab Invest. 1980 Jan;42(1):91–96. [PubMed] [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Traub P., Nelson W. J., Kühn S., Vorgias C. E. The interaction in vitro of the intermediate filament protein vimentin with naturally occurring RNAs and DNAs. J Biol Chem. 1983 Feb 10;258(3):1456–1466. [PubMed] [Google Scholar]
  21. Velasco M. E., Roessmann U., Gambetti P. The presence of glial fibrillary acidic protein in the human pituitary gland. J Neuropathol Exp Neurol. 1982 Mar;41(2):150–163. doi: 10.1097/00005072-198203000-00005. [DOI] [PubMed] [Google Scholar]
  22. Ward W. S., Schmidt W. N., Schmidt C. A., Hnilica L. S. Association of cytokeratin p39 with DNA in intact Novikoff hepatoma cells. Proc Natl Acad Sci U S A. 1984 Jan;81(2):419–423. doi: 10.1073/pnas.81.2.419. [DOI] [PMC free article] [PubMed] [Google Scholar]

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