Table 2. LF fluxes following DMSO and BAY treatment.
| Type | Pathway | ID | Reaction | DMSO* | BAY† | Ratio | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Flux | LB | UB | Flux | LB | UB | |||||
| NET | Transport | GLUT | GLC.x → GLC | 6.12E+02 | 6.12E+02 | 6.12E+02 | 8.80E+02 | 8.80E+02 | 8.80E+02 | 1.44 |
| PYRR | PYR.x → PYR.c | 9.98E+01 | 9.95E+01 | 1.01E+02 | 6.06E+01 | 6.06E+01 | 6.06E+01 | 0.61 | ||
| MCT | LAC ↔ LAC.x | 8.19E+02 | 8.17E+02 | 8.20E+02 | 1.33E+03 | 1.33E+03 | 1.33E+03 | 1.62 | ||
| ALAR | ALA → ALA.x | 2.67E+00 | 2.36E+00 | 3.29E+00 | 5.98E+00 | 5.88E+00 | 6.24E+00 | 2.24 | ||
| GLNR | GLN.x → GLN | 3.78E+01 | 3.77E+01 | 3.79E+01 | 2.06E+01 | 2.06E+01 | 2.06E+01 | 0.54 | ||
| GLUR | GLU ↔ GLU.x | 1.61E+01 | 1.56E+01 | 1.62E+01 | 1.68E+01 | 1.68E+01 | 1.68E+01 | 1.05 | ||
| ASPR | ASP → ASP.x | 2.36E+00 | 2.32E+00 | 2.49E+00 | 1.80E+00 | 1.80E+00 | 1.81E+00 | 0.76 | ||
| SERR | SER.x → SER | 1.03E+01 | 1.03E+01 | 1.06E+01 | 2.50E+00 | 2.50E+00 | 2.50E+00 | 0.24 | ||
| CYSR | CYX.x → CYS+CYS | 2.79E+00 | 2.79E+00 | 2.95E+00 | 3.07E-01 | 3.06E-01 | 3.07E-01 | 0.11 | ||
| GLYR | GLY → GLY.x | 2.52E+00 | 2.30E+00 | 2.73E+00 | 5.52E-01 | 4.30E-01 | 7.45E-01 | 0.22 | ||
| Glycolysis | HK | GLC → G6P | 6.12E+02 | 6.12E+02 | 6.12E+02 | 8.80E+02 | 8.80E+02 | 8.80E+02 | 1.44 | |
| PGI | G6P ↔ F6P | 6.09E+02 | 6.08E+02 | 6.09E+02 | 8.42E+02 | 8.42E+02 | 8.42E+02 | 1.38 | ||
| PFK | F6P → FBP | 6.07E+02 | 6.07E+02 | 6.07E+02 | 8.65E+02 | 8.65E+02 | 8.65E+02 | 1.43 | ||
| ALDO | FBP ↔ DHAP+GAP | 6.07E+02 | 6.07E+02 | 6.07E+02 | 8.65E+02 | 8.65E+02 | 8.65E+02 | 1.43 | ||
| TPI | DHAP ↔ GAP | 6.06E+02 | 6.06E+02 | 6.06E+02 | 8.65E+02 | 8.65E+02 | 8.65E+02 | 1.43 | ||
| GAPDH | GAP ↔ 3PG | 1.21E+03 | 1.21E+03 | 1.21E+03 | 1.74E+03 | 1.74E+03 | 1.74E+03 | 1.44 | ||
| ENO | 3PG → PEP | 1.21E+03 | 1.21E+03 | 1.21E+03 | 1.57E+03 | 1.57E+03 | 1.57E+03 | 1.3 | ||
| PK | PEP → PYR.c | 1.23E+03 | 1.19E+03 | 1.23E+03 | 1.65E+03 | 1.65E+03 | 1.65E+03 | 1.34 | ||
| LDH | PYR.c ↔ LAC | 8.19E+02 | 8.17E+02 | 8.20E+02 | 1.33E+03 | 1.33E+03 | 1.33E+03 | 1.62 | ||
| GPT1 | PYR.c ↔ ALA | 9.62E+00 | 9.44E+00 | 9.62E+00 | 9.36E+00 | 9.32E+00 | 9.42E+00 | 0.97 | ||
| GPT2 | PYR.m ↔ ALA | 1.14E-01 | 2.28E-07 | –1.22E-05 | 6.41E-04 | |||||
| Pentose phosphate pathway | G6PD | G6P → P5P+CO2 | 2.02E-02 | 0.00E+00 | 1.08E+00 | 3.64E+01 | 3.64E+01 | 3.64E+01 | 1801.98 | |
| TK1 | P5P+P5P ↔ S7P+GAP | –9.06E-01 | –9.28E-01 | –9.06E-01 | 1.17E+01 | 1.17E+01 | 1.17E+01 | –12.89 | ||
| TA | S7P+GAP ↔ F6P+E4P | –9.06E-01 | –9.28E-01 | –9.06E-01 | 1.17E+01 | 1.17E+01 | 1.17E+01 | –12.89 | ||
| TK2 | P5P+E4P ↔ F6P+GAP | –9.06E-01 | –9.28E-01 | –9.06E-01 | 1.17E+01 | 1.17E+01 | 1.17E+01 | –12.89 | ||
| Anaplerosis | PYRT | PYR.c → PYR.m | 4.99E+02 | 4.97E+02 | 4.99E+02 | 5.50E+02 | 5.50E+02 | 5.50E+02 | 1.1 | |
| PC | PYR.m+CO2 → OAC | 2.11E+01 | 2.07E+01 | 2.17E+01 | 9.05E+01 | 9.05E+01 | 9.05E+01 | 4.28 | ||
| PEPCK | OAC → PEP+CO2 | 1.36E+01 | 1.36E+01 | 1.37E+01 | 8.58E+01 | 8.58E+01 | 8.58E+01 | 6.31 | ||
| ME2 | MAL → PYR.m+CO2 | 1.30E+01 | 1.28E+01 | 1.37E+01 | 1.00E-07 | 0.00E+00 | 9.49E-06 | 0 | ||
| ME1 | MAL → PYR.c+CO2 | 3.20E-03 | 0.00E+00 | 1.73E+00 | 1.00E-07 | 0.00E+00 | 2.15E-05 | |||
| FAO | FAO → AcCoA.m | 1.00E-07 | 0.00E+00 | 3.48E+00 | 1.09E-04 | 8.34E-06 | 4.14E-02 | |||
| GLDH | GLU ↔ AKG | 1.33E+01 | 1.31E+01 | 1.35E+01 | –2.46E-01 | –2.47E-01 | –2.46E-01 | –0.02 | ||
| GLS | GLN ↔ GLU | 3.40E+01 | 3.35E+01 | 3.42E+01 | 1.88E+01 | 1.88E+01 | 1.88E+01 | 0.55 | ||
| Tricarboxylic acid cycle | PDH | PYR.m → AcCoA.m+CO2 | 4.90E+02 | 4.90E+02 | 4.92E+02 | 4.60E+02 | 4.60E+02 | 4.60E+02 | 0.94 | |
| CS | AcCoA.m+OAC → CIT | 4.90E+02 | 4.84E+02 | 4.91E+02 | 4.60E+02 | 4.60E+02 | 4.60E+02 | 0.94 | ||
| IDH | CIT ↔ AKG+CO2 | 2.70E+01 | 2.70E+01 | 2.76E+01 | 1.45E+01 | 1.45E+01 | 1.45E+01 | 0.54 | ||
| OGDH | AKG → SUC+CO2 | 4.03E+01 | 3.99E+01 | 4.04E+01 | 1.43E+01 | 1.43E+01 | 1.43E+01 | 0.35 | ||
| SDH | SUC ↔ FUM | 4.03E+01 | 3.99E+01 | 4.04E+01 | 1.43E+01 | 1.43E+01 | 1.43E+01 | 0.35 | ||
| FH | FUM ↔ MAL | 4.03E+01 | 3.99E+01 | 4.04E+01 | 1.43E+01 | 1.43E+01 | 1.43E+01 | 0.35 | ||
| MDH | MAL ↔ OAC | 4.91E+02 | 4.91E+02 | 4.92E+02 | 4.60E+02 | 4.60E+02 | 4.60E+02 | 0.94 | ||
| GOT | OAC ↔ ASP | 7.91E+00 | 7.76E+00 | 7.98E+00 | 4.46E+00 | 4.46E+00 | 4.46E+00 | 0.56 | ||
| Amino acid metabolism | PST | 3PG → SER | 4.03E-01 | 3.74E-01 | 5.04E-01 | 1.73E+02 | 1.73E+02 | 1.73E+02 | 429.83 | |
| SHT | SER ↔ GLY+MEETHF | 6.63E+00 | 6.59E+00 | 6.65E+00 | 2.85E+00 | 2.79E+00 | 2.93E+00 | 0.43 | ||
| CYST | SER ↔ CYS | –3.88E+00 | –3.91E+00 | –3.87E+00 | 2.03E-01 | 2.02E-01 | 2.03E-01 | –0.05 | ||
| SD | SER → PYR.c | 2.80E+00 | 2.80E+00 | 2.80E+00 | 1.70E+02 | 1.70E+02 | 1.70E+02 | 60.81 | ||
| GLYS | CO2+MEETHF → GLY | 3.63E+00 | 3.50E+00 | 3.65E+00 | 1.41E+00 | 1.30E+00 | 1.46E+00 | 0.39 | ||
| Biomass | BIOMASS | 1216*AcCoA.c+295.6 *ALA +232.4*ASP+114.7*CO2+71.43*CYS+57.14*DHAP+142.4*G6P+ 158.6*GLN+190.1*GLU +324.2*GLY+125.6*MEETHF+114.7*P5P+217.2 *SER → biomass |
2.39E-02 | 2.39E-02 | 2.50E-02 | 1.14E-02 | 1.14E-02 | 1.14E-02 | 0.48 | |
| ACL | CIT → AcCoA.c+MAL | 4.63E+02 | 4.63E+02 | 4.66E+02 | 4.45E+02 | 4.45E+02 | 4.45E+02 | 0.96 | ||
| LIPS | AcCoA.c → lipid | 4.34E+02 | 4.29E+02 | 4.34E+02 | 4.32E+02 | 4.32E+02 | 4.32E+02 | |||
| Mixing | cPYR | 0*PYR.c → PYR.ms | 1.00E+00 | 9.99E-01 | 1.00E+00 | 1.00E-07 | 0.00E+00 | 1.00E+00 | ||
| mPYR | 0*PYR.m → PYR.ms | 1.00E-07 | 0.00E+00 | 9.83E-04 | 1.00E+00 | 0.00E+00 | 1.00E+00 | |||
| sPYR | PYR.ms → PYR.fix | 1.00E+00 | 1.00E+00 | 1.00E+00 | 1.00E+00 | 1.00E+00 | 1.00E+00 | |||
| EXCH | Transport | MCT | LAC ↔ LAC.x | 6.24E-04 | 0.00E+00 | 3.56E+00 | 7.11E+02 | 7.11E+02 | 7.11E+02 | 1139423.08 |
| GLUR | GLU ↔ GLU.x | 5.06E+00 | 4.82E+00 | 5.75E+00 | 3.48E+00 | 3.48E+00 | 3.48E+00 | 0.69 | ||
| Glycolysis | PGI | G6P ↔ F6P | 1.40E+06 | 1.39E+06 | Inf | 4.31E+06 | 4.31E+06 | 4.31E+06 | ||
| ALDO | FBP ↔ DHAP+GAP | 2.38E+02 | 2.38E+02 | 2.38E+02 | 1.02E+03 | 1.02E+03 | 1.02E+03 | 4.28 | ||
| TPI | DHAP ↔ GAP | 9.99E+06 | Inf | 7.57E+03 | 7.57E+03 | 7.57E+03 | ||||
| GAPDH | GAP ↔ 3PG | 5.81E+02 | 5.81E+02 | 7.25E+02 | 1.09E+02 | 1.07E+02 | 1.09E+02 | 0.19 | ||
| LDH | PYR.c ↔ LAC | 2.65E+03 | 2.58E+03 | 2.65E+03 | 4.92E+01 | 4.91E+01 | 4.94E+01 | 0.02 | ||
| GPT1 | PYR.c ↔ ALA | 1.00E-07 | 0.00E+00 | 5.60E-02 | 2.45E+03 | 2.45E+03 | 2.45E+03 | 24500000000 | ||
| GPT2 | PYR.m ↔ ALA | 1.00E-07 | 0.00E+00 | 5.65E-02 | 1.00E-07 | 0.00E+00 | 1.20E-05 | |||
| Pentose phosphate pathway | TK1 | P5P+P5P ↔ S7P+GAP | 1.28E+06 | 9.01E+03 | Inf | 1.00E+07 | -Inf | Inf | ||
| TA | S7P+GAP ↔ F6P+E4P | 8.89E+00 | 8.88E+00 | 9.53E+00 | 5.10E+01 | 5.10E+01 | 5.10E+01 | 5.74 | ||
| TK2 | P5P+E4P ↔ F6P+GAP | 6.93E+00 | 5.12E+00 | 6.98E+00 | 1.00E-07 | 0.00E+00 | 1.56E-04 | 0 | ||
| Anaplerosis | GLDH | GLU ↔ AKG | 5.63E+03 | 4.43E+03 | 5.66E+03 | 1.42E+03 | 1.42E+03 | 1.42E+03 | 0.25 | |
| GLS | GLN ↔ GLU | 1.27E+00 | 1.20E+00 | 1.50E+00 | 5.52E-01 | 5.51E-01 | 5.55E-01 | 0.43 | ||
| Tricarboxylic acid cycle | IDH | CIT ↔ AKG+CO2 | 3.36E+00 | 3.24E+00 | 3.92E+00 | 4.66E+00 | 4.66E+00 | 4.66E+00 | 1.39 | |
| SDH | SUC ↔ FUM | 4.30E+02 | 4.30E+02 | 1.46E+06 | 1.04E+04 | 1.04E+04 | 1.04E+04 | |||
| FH | FUM ↔ MAL | 7.29E+06 | -Inf | Inf | 4.56E+06 | 4.56E+06 | 4.56E+06 | |||
| MDH | MAL ↔ OAC | 5.49E+02 | 5.47E+02 | 5.49E+02 | 1.00E-07 | 0.00E+00 | 6.30E-03 | 0 | ||
| GOT | OAC ↔ ASP | 1.04E+02 | 1.04E+02 | 1.04E+02 | 4.76E+05 | 4.76E+05 | 4.76E+05 | 4576.92 | ||
| Amino acid metabolism | SHT | SER ↔ GLY+MEETHF | 1.39E+00 | 1.37E+00 | 1.41E+00 | 1.86E+03 | 1.86E+03 | 1.86E+03 | 1338.13 | |
| CYST | SER ↔ CYS | 1.25E-07 | 0.00E+00 | 4.22E-02 | 1.33E-01 | 1.33E-01 | 1.33E-01 | 1064000 | ||
*
SSR 393.5 [311.2–416.6] (95% CI, 362 DOF).
†
SSR 392.4 [308.4–413.4] (95% CI, 359 DOF).