Erratum - Transcription factor nuclear factor erythroid 2 p45-related factor 2 (NRF2) ameliorates sepsis-associated acute kidney injury by maintaining mitochondrial homeostasis and improving the mitochondrial function

ERRATUM

This corrects the article published in European Journal of Histochemistry 2022;66:3412, in which we discovered some errors in Figure 1 and Figure 3.

To better report the results, Figure 1 has been replaced by an immunofluorescence diagram, and the sorting in Figure 1 has been adjusted.

The wrong Nucleus NRF2 Western blot band is used in Figure 3B, which needs to be corrected. The correct Figure 1 and Figure 3 and relevant legends are shown below.

Figure 1.

Effect of LPS on NRF-2 activation in NRK-52e cells. A) Cell viability after addition of 6-50 μg/mL LPS for 24 h (n=5). B) Cell viability after addition of 50 μg/mL LPS for 6-24 h (n=5). C) Western blots showing NRF2 expression in cells at 6, 12, and 24 h after addition of LPS (50 μg/mL). D) Quantitative analysis of the Western blot data, with expression relative to β-actin (n=3). E) Immunofluorescence detection of the expression and distribution of NRF2 following LPS treatment. Data are presented as means ± SD; *p<0.05 vs control.

Figure 3.

Effect of NRF2 on the inflammatory response and oxidative stress in LPS-induced injury of NRK-52e cells. A) Immunoblotting of NRF2 following transfection with LV-NRF2. B) Western blotting of NRF2, Nucleus NRF2, p-p65, IKB-α, p-p38 and erk. C) Quantitative analysis of the Western blotting data, with expression relative to β-actin, p65, p38 or Lamin B1 (n=3). D, E) ELISA of TNF-α and IL-6 in cell culture supernatants (n=5). F) SOD activity (n=5). G) MDA level (n=5). H) Representative fluorescence microscopy images of ROS production (DCFH-DA probe). I) Quantitative analysis of the ROS data (n=5). Data are presented as means ± SD; *p<0.05 vs control, #p<0.05 vs LPS.