Monoclonal (mAb) F6.30C5.11A6 does not react with recombinant Pfs47. A) Schematic of the three Pfs47 6-cys domains included in the 7 E. coli-produced recombinant proteins tested for antibody recognition using ELISA. B) The reactivity of the 6 antibodies, JH11 (▪), mAb1 (▪), mAb5 (▪), IB2 (▪), mIgG (▪), 11A6 (▪) against the indicated recombinant Pfs47 proteins (1–7) is plotted. Alkaline phosphatase-labeled anti-mouse secondary antibody and p-nitrophenyl disodium phosphate substrate were used for detection.