Table 1:
Standard membrane feed using serum collected 1 week after the 3rd injection (Day 50)
| Day 50 | 1:20 dilution | |||||||
|---|---|---|---|---|---|---|---|---|
| Mouse | Mosquito Inf/dissect | Oocyst | % Inhib | |||||
| Ave | Range | |||||||
| α-GAMa ms1 | 2/20 | 0.1 | (0–1) | 99.5 | ||||
| α-GAM ms2 | 1/20 | 0.05 | (0–1) | 99.7 | ||||
| α-GAM ms3 | 2/20 | 0.1 | (0–1) | 99.5 | 1:100 dilution | |||
| α-GAM ms4 | 1/20 | 0.15 | (0–3) | 99.2 | Mosquito Inf/dissect | Oocyst | % Inhib | |
| α-GAM ms5 | 3/20 | 0.15 | (0–1) | 99.2 | Ave | Range | ||
| α-mem ms1 | 1/20 | 0.05 | (0–1) | 99.7 | 5/20 | 0.25 | 0–2 | 99.3 |
| α-mem ms2 | 0/20 | 0 | 0 | 100.0 | 2/20 | 0.20 | 0–3 | 99.5 |
| α-mem ms3 | 0/20 | 0 | 0 | 100.0 | 12/20 | 1.50 | 0–11 | 96.0 |
| α-mem ms4 | 0/20 | 0 | 0 | 100.0 | 6/20 | 0.30 | 0–1 | 99.2 |
| α-mem ms5 | 0/20 | 0 | 0 | 100.0 | 11/20 | 1.40 | 0–6 | 96.3 |
| α-ippt ms1 | 0/20 | 0 | 0 | 100.0 | 2/20 | 0.10 | 0–1 | 99.7 |
| α-ippt ms2 | 0/20 | 0 | 0 | 100.0 | 5/20 | 0.50 | 0–3 | 98.7 |
| α-ippt ms3 | 0/20 | 0 | 0 | 100.0 | 4/20 | 0.35 | 0–3 | 99.1 |
| α-ippt ms4 | 0/20 | 0 | 0 | 100.0 | 1/20 | 0.05 | 0–1 | 99.9 |
| α-ippt ms5 | 0/20 | 0 | 0 | 100.0 | 7/20 | 0.70 | 0–5 | 98.1 |
| Prebleed | 19/20 | 18.4 | (0–52) | 0 | 18/20 | 37.60 | 0–94 | 0.0 |
| 20/20 | 37.60 | 6–112 | 0.0 | |||||
| mAb 4B7 | 16/20 | 3 | (0–8) | 86.1 | 9/20 | 2.05 | 0–9 | 95.3 |
| 9/20 | 1.05 | 0–5 | 97.1 | |||||
| Human serum | 20/20 | 21.65 | (1–54) | 0 | 20/20 | 44.30 | 1–81 | 0.0 |
α-GAM indicates mice immunized with whole gametes/zygotes, α-mem indicates mice immunized with gamete/zygote membranes, and α-ippt indicates mice immunized with mAb immunoprecipitate. Mice were immunized 3 times with decreasing concentrations of gametes/zygotes. 2 x 107 gametes/zygotes / mouse or the equivalent amount of membranes or immunoprecipitated material was used for the first injection, 1 x 107 gametes/zygotes / mouse for the second injection, and 5 x 106 gametes/zygotes / mouse for the third injection. The immunogen was emulsified in monophosphoryl Lipid A and trehalose dicorynomycolate in 2% oil (squalene)-Tween® 80-water (MPL-TDM) (Sigma, St Louis, MO) immediately before injection into mice.