Table 1.

Generation of transgenic clonal raider ants expressing GCaMP6s.

Treatment $\frac{\frac{\mathit{\text{fmol}}}{\mu L}\mathit{\text{DNA}}}{\frac{ng}{\mu L}\mathit{\text{RNA}}}$	Egg age at injection (hours)	# eggs injected	# G0 eggs hatched	# G0 adults eclosed	# G0 adults with fluorescence	Minimum # of lines generated	Overall efficiency	Transformation efficiency
27.8/110	<5	1945	155 (8.0%)	14	3	1	0.00021	0.026
27.8/220	<5	1367	72 (5.3%)	16	0
27.8/440	<5	739	6 (0.8%)	0	0
27.8/110	<3	637	15 (2.4%)	8	5
27.8/110	<3	353	44 (12.5%)	17	2	1	0.0028	0.059

The “Treatment” column indicates injection mix concentrations of plasmid DNA and transposase mRNA. G0 adults from the first four treatments were reared as a group, and we therefore cannot determine which treatment generated the line that was propagated from that group. Overall efficiency was calculated by dividing the minimum number of lines generated by the number of eggs injected; transformation efficiency was calculated by dividing the minimum number of lines generated by the number of G0 adults eclosed.