Skip to main content
. 2023 Jun 21;619(7969):394–402. doi: 10.1038/s41586-023-06257-4

Fig. 1. PIWI proteins bind sites containing or lacking canonical seed pairing.

Fig. 1

a, Small RNA guides direct eukaryotic Argonaute proteins to complementary targets. nt, nucleotide. b, Binding affinities (Kd in pM) of MIWI, MILI, EfPiwi and mouse AGO2 loaded with piRNA-1 for canonical and non-canonical target sites. c, Left, MIWI, MILI, EfPiwi and mouse AGO2 binding affinities for targets contiguously paired from nucleotide g2. Right, relationship between binding energy ΔG0 calculated from Kd (mean of three independent trials) and predicted binding energy ΔG0. Goodness-of-fit for linear regression (r2) and P value for two-tailed permutation test for Pearson’s correlation are shown. All data are in Supplementary Fig. 2a. d, MIWI, MILI, EfPiwi and mouse AGO2 binding affinities for nine-nucleotide complementary stretches contiguously paired from all guide nucleotides. All data are in Supplementary Fig. 2b. Mean and standard deviation of data from three independent trials are shown (b,c (left), d).