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. 2023 Jun 21;619(7969):394–402. doi: 10.1038/s41586-023-06257-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a, Pre-steady-state cleavage rate in absence or presence of mouse GTSF1 for mouse AGO2, MILI and MIWI, and EmGtsf1 for EfPiwi. b, Acceleration of pre-steady-state cleavage rates by mouse GTSF1 for MILI and MIWI, and by EmGtsf1 for EfPiwi. Data are for four different piRNA guide sequences bound to MILI or MIWI and for two piRNA guide sequences bound to EfPiwi (Extended Data Fig. 1c). Box plots show IQR and median; 95% confidence interval was calculated with 10,000 bootstrapping iterations.