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. 2023 Jun 22;15(13):2839. doi: 10.3390/nu15132839

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of 3T3-L1 adipocyte-derived conditioned medium (CM) on PLK1 expression and phosphorylation in BC cells. Immunoblotting showing total protein PLK1 (a) and phosphorylated (p) PLK1 (b) levels in MCF-7 BC cells after 48 and 72 h exposure to conditioned medium. Immunoblotting showing total protein PLK1 (c) and phosphorylated (p) PLK1 (d) levels in MDA-MB-231 BC cells treated as indicated. GAPDH was used as a control for equal loading and transfer. Italicized numbers below the blot represent the mean of the band optical density expressed as fold over CM-untreated BC cells.