Extended Data Fig. 5 |. Subchronic treatment with a ketone ester induces ketosis.

a, Absolute quantification of R-βHB in brain tissue of 22 month male and female wild-type mice fed for 7 days with control diet or BH-BD. b, Schematic of subchronic BH-BD and control treatment schedule. c, Quantification of animal body weight at 0800 daily. d, Quantification of blood glucose concentrations 1 hour post oral gavage. e, Quantification of plasma βHB concentrations 1 hour post oral gavage.

a, Mean ± S.E.M, N=13, p-value calculated using Kolmogorov-Smirnov test.

c, Mean ± S.E.M, N=5–6, p-value calculated using mixed-effects analysis with Sidak’s multiple comparison test.

d, Mean ± S.E.M, N=5–6, p-value calculated using mixed-effects analysis with Sidak’s multiple comparison test.

e, Mean ± S.E.M, N=5–6, p-value calculated using mixed-effects analysis with Sidak’s multiple comparison test.