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[Preprint]. 2023 Jul 5:2023.07.04.547745. [Version 1] doi: 10.1101/2023.07.04.547745

Figure 3. VPS50 mKO neurons show a reduction in synaptic activity that can be recovered by inducing synaptic vesicle acidification.

Figure 3.

(A) Representative traces and (B-C) quantification of Amplitude (B) and Frequency (C) of spontaneous AMPA-mediated EPSCs. (D) Representative traces and (E-F) quantification of the frequency (E) and amplitude (F) of miniature AMPA-mediated currents. (G) Current clamp of Control and VPS50 mKO neurons. (H) Quantification of the frequency of spiking in current clamp. (I-K) Current clamp representative traces of Control and VPS50 mKO neurons. Blue bars show the period pHoenix is activated. (K) Quantification of the frequency of spiking of Control and VPS50 mKO neurons before, during and after stimulus with pHoenix to acidify synaptic vesicles. At least 18 neurons from 3 independent experiments were analyzed. *** p<0.001.