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[Preprint]. 2023 Jul 3:2023.07.03.547580. [Version 1] doi: 10.1101/2023.07.03.547580

Figure 3. Unloaded Actin Sliding Velocity.

Figure 3.

(A) In vitro motility rates for myosin S1 constructs were measured using TRITC-labeled actin filaments. Average actin sliding velocity was calculated from actin filaments that were ≥1 μm in length and mobile in >15 frames per video. (B) Summary values for In vitro motility actin sliding velocity assay. Values are an average of three separate purifications per S1 construct, and 7–9 videos per construct. % motile was calculated from the ratio of actin filaments moving in >15 frames relative to total number of actin filaments tracked. Values are reported as average ± standard error. A one-way ANOVA was used to assess statistical significance (p<0.05) compared to β-MHC WT S1. N = 7–8 videos per myosin S1 construct.