Skip to main content
. 2023 Feb 22;19(8):2372–2385. doi: 10.1080/15548627.2023.2178159

Figure 1.

Figure 1.

Adapting the PCA platform for detecting PPI inhibition by small compounds. (A) Scheme of the PCA methodology. ATG12 and ATG3 proteins are fused at their C termini to inactive fragments of luciferase GLuc1 (L1) and GLuc2 (L2), respectively, and co-expressed in cells [31]. When ATG3 and ATG12 proteins interact, the inactive fragments combine to form fully active luciferase. (B) Representative PCA assay of ATG12-L1 and ATG3-L2 lysate mixing protocol. ATG12-L1 WT or ATG12-L1K54D lysates were mixed with ATG3-L2 lysates. RLU, relative luminescence units. (C) Representative PCA assay of BCL2L1-L1 and BAX-L2 lysate mixing protocol with ABT-737. BCL2L1-L1 expressing lysate was mixed with 1 µM ABT-737 prior to addition of BAX-L2 lysate.