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. 1991 Feb;48(2):116–121. doi: 10.1136/oem.48.2.116

Increase in numbers of CD8 positive lymphocytes and eosinophils in peripheral blood of subjects with late asthmatic reactions induced by toluene diisocyanate.

S Finotto 1, L M Fabbri 1, V Rado 1, C E Mapp 1, P Maestrelli 1
PMCID: PMC1035331  PMID: 1847821

Abstract

Occupational asthma induced by toluene diisocyanate (TDI) shares several features with allergic asthma, but the mechanism of action of TDI is poorly understood. Ten sensitised subjects, previously shown to develop a dual or late asthmatic reaction after inhaling TDI were examined. In each subject, forced expiratory volume in one second (FEV1) was measured and venous blood was taken before, and 30 minutes and eight, 24, 48, and 72 hours after exposure to TDI (0.005-0.015 ppm for 10-30 minutes). Filtered air was used as a control. Differential leucocyte counts were determined and phenotypic analysis was performed by immunofluorescence on mononuclear cells using monoclonal antibodies (anti-CD3, anti-CD4, anti-CD8, and anti-HLA-DR). Five subjects developed a dual asthmatic reaction and five had a late reaction. Percentage of CD8 positive lymphocytes increased significantly eight hours after exposure to TDI (from 27 +/- 3 (SEM) % to 42.1 +/- 5%) in the subjects with an isolated late reaction. A delayed significant further increase in suppressor/cytotoxic T lymphocytes was seen in seven of the 10 subjects 48 hours after active exposure (from 27 +/- 2% to 42 +/- 4.8%), irrespective of the type of asthmatic reaction developed after exposure to TDI. Eosinophil percentage increased from 2.5% +/- 1.0 to 6.4% +/- 1.2 24 hours after exposure to TDI and the increase was sustained for up to 48 hours (4.7 +/- 1.1%). No significant variations of FEV1 or cell percentages were seen in the controls. In conclusion, the events triggered by exposure to TDI in sensitised subjects included changes in lung function and systemic effects which lasted longer than bonchoconstriction and concerned suppressor/cytotoxic lymphocytes and eosinophils. These results suggest that TDI induced late asthmatic reactions may be associated with an immunological response to TDI or to its products.

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Selected References

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  1. Bernstein I. L. Isocyanate-induced pulmonary diseases: a current perspective. J Allergy Clin Immunol. 1982 Jul;70(1):24–31. doi: 10.1016/0091-6749(82)90197-x. [DOI] [PubMed] [Google Scholar]
  2. Booij-Noord H., de Vries K., Sluiter H. J., Orie N. G. Late bronchial obstructive reaction to experimental inhalation of house dust extract. Clin Allergy. 1972 Mar;2(1):43–61. doi: 10.1111/j.1365-2222.1972.tb01267.x. [DOI] [PubMed] [Google Scholar]
  3. Boschetto P., Fabbri L. M., Zocca E., Milani G., Pivirotto F., Dal Vecchio A., Plebani M., Mapp C. E. Prednisone inhibits late asthmatic reactions and airway inflammation induced by toluene diisocyanate in sensitized subjects. J Allergy Clin Immunol. 1987 Sep;80(3 Pt 1):261–267. doi: 10.1016/0091-6749(87)90028-5. [DOI] [PubMed] [Google Scholar]
  4. Butcher B. T., O'Neil C. E., Reed M. A., Salvaggio J. E. Radioallergosorbent testing of toluene diisocyanate-reactive individuals using p-tolyl isocyanate antigen. J Allergy Clin Immunol. 1980 Sep;66(3):213–216. doi: 10.1016/0091-6749(80)90041-x. [DOI] [PubMed] [Google Scholar]
  5. Cartier A., Grammer L., Malo J. L., Lagier F., Ghezzo H., Harris K., Patterson R. Specific serum antibodies against isocyanates: association with occupational asthma. J Allergy Clin Immunol. 1989 Oct;84(4 Pt 1):507–514. doi: 10.1016/0091-6749(89)90364-3. [DOI] [PubMed] [Google Scholar]
  6. Durham S. R., Carroll M., Walsh G. M., Kay A. B. Leukocyte activation in allergen-induced late-phase asthmatic reactions. N Engl J Med. 1984 Nov 29;311(22):1398–1402. doi: 10.1056/NEJM198411293112202. [DOI] [PubMed] [Google Scholar]
  7. Durham S. R., Kay A. B. Eosinophils, bronchial hyperreactivity and late-phase asthmatic reactions. Clin Allergy. 1985 Sep;15(5):411–418. doi: 10.1111/j.1365-2222.1985.tb02290.x. [DOI] [PubMed] [Google Scholar]
  8. Fabbri L. M., Boschetto P., Zocca E., Milani G., Pivirotto F., Plebani M., Burlina A., Licata B., Mapp C. E. Bronchoalveolar neutrophilia during late asthmatic reactions induced by toluene diisocyanate. Am Rev Respir Dis. 1987 Jul;136(1):36–42. doi: 10.1164/ajrccm/136.1.36. [DOI] [PubMed] [Google Scholar]
  9. Fabbri L. M., Chiesura-Corona P., Dal Vecchio L., di Giacomo G. R., Zocca E., de Marzo N., Maestrelli P., Mapp C. E. Prednisone inhibits late asthmatic reactions and the associated increase in airway responsiveness induced by toluene-diisocyanate in sensitized subjects. Am Rev Respir Dis. 1985 Nov;132(5):1010–1014. doi: 10.1164/arrd.1985.132.5.1010. [DOI] [PubMed] [Google Scholar]
  10. Gerblich A. A., Campbell A. E., Schuyler M. R. Changes in T-lymphocyte subpopulations after antigenic bronchial provocation in asthmatics. N Engl J Med. 1984 May 24;310(21):1349–1352. doi: 10.1056/NEJM198405243102103. [DOI] [PubMed] [Google Scholar]
  11. Gonzalez M. C., Diaz P., Galleguillos F. R., Ancic P., Cromwell O., Kay A. B. Allergen-induced recruitment of bronchoalveolar helper (OKT4) and suppressor (OKT8) T-cells in asthma. Relative increases in OKT8 cells in single early responders compared with those in late-phase responders. Am Rev Respir Dis. 1987 Sep;136(3):600–604. doi: 10.1164/ajrccm/136.3.600. [DOI] [PubMed] [Google Scholar]
  12. Mapp C. E., Boschetto P., Dal Vecchio L., Maestrelli P., Fabbri L. M. Occupational asthma due to isocyanates. Eur Respir J. 1988 Mar;1(3):273–279. [PubMed] [Google Scholar]
  13. Mapp C. E., Polato R., Maestrelli P., Hendrick D. J., Fabbri L. M. Time course of the increase in airway responsiveness associated with late asthmatic reactions to toluene diisocyanate in sensitized subjects. J Allergy Clin Immunol. 1985 May;75(5):568–572. doi: 10.1016/0091-6749(85)90031-4. [DOI] [PubMed] [Google Scholar]
  14. Metzger W. J., Zavala D., Richerson H. B., Moseley P., Iwamota P., Monick M., Sjoerdsma K., Hunninghake G. W. Local allergen challenge and bronchoalveolar lavage of allergic asthmatic lungs. Description of the model and local airway inflammation. Am Rev Respir Dis. 1987 Feb;135(2):433–440. doi: 10.1164/arrd.1987.135.2.433. [DOI] [PubMed] [Google Scholar]
  15. Nagaya H. Induction of antigen-specific suppressor cells in patients with hay fever receiving immunotherapy. J Allergy Clin Immunol. 1985 Mar;75(3):388–394. doi: 10.1016/0091-6749(85)90077-6. [DOI] [PubMed] [Google Scholar]
  16. O'Brien I. M., Newman-Taylor A. J., Burge P. S., Harries M. G., Fawcett I. W., Pepys J. Toluene di-isocyanate-induced asthma. II. Inhalation challenge tests and bronchial reactivity studies. Clin Allergy. 1979 Jan;9(1):7–15. doi: 10.1111/j.1365-2222.1979.tb01517.x. [DOI] [PubMed] [Google Scholar]
  17. Paggiaro P. L., Filieri M., Loi A. M., Roselli M. G., Cantalupi R., Parlanti A., Toma G., Baschieri L. Absence of IgG antibodies to TDI-HSA in a radioimmunological study. Clin Allergy. 1983 Jan;13(1):75–79. doi: 10.1111/j.1365-2222.1983.tb02570.x. [DOI] [PubMed] [Google Scholar]
  18. Patterson R., Hargreave F. E., Grammer L. C., Harris K. E., Dolovich J. Toluene diisocyanate respiratory reactions. I. Reassessment of the problem. Int Arch Allergy Appl Immunol. 1987;84(1):93–100. doi: 10.1159/000234404. [DOI] [PubMed] [Google Scholar]
  19. Rocklin R. E., Sheffer A. L., Greineder D. K., Melmon K. L. Generation of antigen-specific suppressor cells during allergy desensitization. N Engl J Med. 1980 May 29;302(22):1213–1219. doi: 10.1056/NEJM198005293022201. [DOI] [PubMed] [Google Scholar]
  20. Takatsu K., Ishizaka K. Reaginic antibody formation in the mouse. VII. Induction of suppressor T cells for IgE and IgG antibody responses. J Immunol. 1976 May;116(5):1257–1264. [PubMed] [Google Scholar]
  21. Zocca E., Fabbri L. M., Boschetto P., Plebani M., Masiero M., Milani G. F., Pivirotto F., Mapp C. E. Leukotriene B4 and late asthmatic reactions induced by toluene diisocyanate. J Appl Physiol (1985) 1990 Apr;68(4):1576–1580. doi: 10.1152/jappl.1990.68.4.1576. [DOI] [PubMed] [Google Scholar]

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