Skip to main content
. 2023 Jun 22;26(7):107192. doi: 10.1016/j.isci.2023.107192
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

4B7 monoclonal antibody LMIV, NIAID, NIH N/A
Coat Anti-mouse IgG1 Southern Biotech. Cat No 1073-04
Coat Anti-mouse IgG2a Southern Biotech. Cat No 1083-04
Coat Anti-mouse IgG2b Southern Biotech. Cat No 1093-04
Coat Anti-mouse IgG2c Southern Biotech. Cat No 1077-04
Coat Anti-mouse IgG3 Southern Biotech. Cat No 1130-04

Biological samples

AB + naive human serum Interstate Blood Bank, Inc., Memphis, TN, USA 1/22/2016
Diagnostic Red Blood Cells Group (O+) Anticoagulant: CPD GRIFOLS BioSupplies, (formerly Interstate Blood Bank, Inc., 5065 Covington Way, Memphis TN, USA; No cat number.

Chemicals, peptides, and recombinant proteins

Pfs25 LMIV, NIAID, NIH Ref #17
Pfs25-EPA LMIV, NIAID, NIH Ref #17
Pfs230D1-EPA LMIV, NIAID, NIH Ref #27
4X NuPAGE™ LDS Sample Buffer ThermoFisher Scientific, Invitrogen Cat: NP0007

Critical commercial assays

Micro BCA Protein Assay Kit ThermoFisher Scientific, Pierce Cat: 23235
ELISpot Flex: Mouse IgG (ALP) (ELISpot reagents and kit) Mabtech, Inc 3825-2A

Experimental models: Organisms/strains

CD-1 mice Charles River https://www.criver.com/products-services/find-model/cd-1r-igs-mouse?region=3611
Anopheles stephensi (Nijmegen) from Joep Meiwissen, The Catholic University of the Netherlands, Neimegen Netherlands and maintained since 1985 at NIH
Plasmodium falciparum, strain NF54(SH) Culture obtained in 2006 from Sanaria

Software and algorithms

Prism GraphPad Software Inc., La Jolla, CA https://www.graphpad.com/
R version 3.6.3. R Core Team and the R Foundation for Statistical Computing version 3.6.3
SoftMax Pro7software SMp7 Molecular Devices SMp7

Other

AS01 Glaxo Smith Kline Supplied by GSK and from SHINGRIX kit A4X57
Alhydrogel® 2% CRODA, Denmark 4883, 5069,
AS04 Glaxo Smith Kline Supplied by GSK
Immuno 4 HBX plates ThermoFisher Scientific Lot 3855
Corning polystyrene flat bottom reading plate Corning Product Number: 3679
Zetasizer Nano-ZS, ZEN 3600 particle size analyzer Malvern Panalytical, Inc. Zetasizer Nano-ZS, ZEN 3600
Immunospot analyzer CTL, Shaker Heights, OH
Spectramax M3 microplate reader Molecular Devices Co Spectramax M3