In the published article, there was an error in the legend for Figure 1, page ten. The accession number of Th. bessarabicum used in the study was indicated erroneously.
Figure 1.
Metaphase cells of Ae. crassa, 4x (A–L) and 6x (M, N), and of Th. bessarabicum (O, P) after FISH with different probe combinations; (A–C) the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL244 + CL258 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (D–F) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL239 + CL232 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (G, H) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL131 + CL219 followed by CL170 + pTa-713; (I) the cell of 4x Ae. crassa AE 742 hybridized with CL232 and CL257; (J–L) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL209 + CL261 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (M, N) – the cell of 6x Ae. crassa AE 131680 hybridized consecutively with CL228 + CL258 followed by pAs1 + pSc119.2; (O, P) – the cell of Th. bessarabicum PI 531711 hybridized with CL193 + CL241 followed by pTa-713. Probe combinations are given on the top of the respective images; probe color corresponds to signal color.
The legend previously stated:
“ Figure 1
Metaphase cells of Ae. crassa, 4x (A–L) and 6x (M,N), and of Th. bessarabicum (O,P) after FISH with different probe combinations; (A–C) the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL244 + CL258 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (D–F) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL239 + CL232 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (G,H) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL131 + CL219 followed by CL170 + pTa-713; (I)- the cell of 4x Ae. crassa AE 742 hybridized with CL232 and CL257; (J–L) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL209 + CL261 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (M,N) – the cell of 6x Ae. crassa AE 131680 hybridized consecutively with CL228 + CL258 followed by pAs1 + pSc119.2; (O,P) – the cell of Th. bessarabicum PI 201890 hybridized with CL193 + CL241 followed by pTa-713. Probe combinations are given on the top of the respective images; probe color corresponds to signal color.”
The corrected legend appears below:
“ Figure 1
Metaphase cells of Ae. crassa, 4x (A–L) and 6x (M,N), and of Th. bessarabicum (O,P) after FISH with different probe combinations; (A–C) the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL244 + CL258 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (D–F) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL239 + CL232 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (G,H) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL131 + CL219 followed by CL170 + pTa-713; (I)- the cell of 4x Ae. crassa AE 742 hybridized with CL232 and CL257; (J–L) - the cell of 4x Ae. crassa AE 742 hybridized consecutively with CL209 + CL261 followed by CL170 + pTa-713 and pAs1 + pSc119.2; (M,N) – the cell of 6x Ae. crassa AE 131680 hybridized consecutively with CL228 + CL258 followed by pAs1 + pSc119.2; (O,P) – the cell of Th. bessarabicum PI 531711 hybridized with CL193 + CL241 followed by pTa-713. Probe combinations are given on the top of the respective images; probe color corresponds to signal color.”
In the published article, there was an error in Table 1, page four. The genomes D1 and D2 were mistyped, the accession number of Th. bessarabicum used in the study was indicated erroneously, and the source of Th. bessarabicum accessions was incorrect.
Table 1.
Plant material.
| Species | 2n | Genome formula | Accessions # | Used in: | Source |
|---|---|---|---|---|---|
| Ae. crassa | 28 | D1D1XcrXcr | AE 742 | Sequencing, qPCR, FISH | IPK, Gatersleben, Germany |
| AE 1649 | FISH | ||||
| K-2485 | FISH | VIR, St.-Petersburg, Russia | |||
| Ae. crassa | 42 | D1D1XcrXcrD2D2 | IG 131680 | qPCR, FISH | ICARDA, Aleppo, Syria |
|
Ae. tauschii subsp. strangulata |
14 | DD | K-112 | Sequencing, qPCR, FISH |
VIR, St.-Petersburg, Russia |
| Ae. tauschii subsp. typica | 14 | DD | K-428 | Sequencing | VIR, St.-Petersburg, Russia |
| Ae. tauschii subsp. tauschii | 14 | DD | K-1619 | FISH | VIR, St.-Petersburg, Russia |
|
Triticum aestivum cv. Chinese Spring |
42 | BBAADD | – | FISH | – |
| Th. bessarabicum | 14 | JbJb | PI 531711 | Sequencing, qPCR, FISH | USDA-ARS GRIN |
The corrected Table 1 appears below.
In the published article there were errors in the Abstract, page one; the Introduction, paragraph two; in Materials and methods, Fluorescense in situ hybridization, paragraph two; in Results, Fluorescence in situ hybridization, paragraphs eight, ten, eleven, fifteen, sixteen, and eighteen; in Discussion, Novel markers for chromosome and genome identification, paragraph seven; in Discussion, Evolutionary changes of repetitive DNA families in Aegilops crassa genome, paragraph five.
The designation of genomic formulas and chromosomes, the subgenomes of Aegilops crassa D1 and D2 were given incorrectly as D (Abdolmalaki et al., 2019) and D (Adams and Wendel, 2005), respectively.
The corrections have been made throughout the text as follows.
In the Abstract, page one, the sentence previously stated:
“It consists of tetraploid (4x) and hexaploid (6x) cytotypes (2n = 4x = 28, D1D (Abdolmalaki et al., 2019) XcrXcr and 2n = 6x = 42, D1D (Abdolmalaki et al., 2019) XcrXcrD2D (Adams and Wendel, 2005), respectively) that are similar morphologically.”
The sentence has been corrected as follows:
“It consists of tetraploid (4x) and hexaploid (6x) cytotypes (2n = 4x = 28, D1D1XcrXcr and 2n = 6x = 42, D1D1XcrXcrD2D2, respectively) that are similar morphologically.”
In the Introduction, paragraph two, the sentence previously stated:
“Ae. crassa consists of tetraploid and hexaploid cytotypes [2n = 4x = 28, D1D (Abdolmalaki et al., 2019) XcrXcr and 2n = 6x = 42, D1D (Abdolmalaki et al., 2019) XcrXcrD (Adams and Wendel, 2005) D (Adams and Wendel, 2005), respectively] that are similar morphologically.”
The sentence has been corrected as follows:
“Ae. crassa consists of tetraploid and hexaploid cytotypes [2n = 4x = 28, D1D1XcrXcr and 2n = 6x = 42, D1D1XcrXcrD2D2, respectively] that are similar morphologically.”
In the Introduction, paragraph two, the sentence previously stated:
“One of the Ae. crassa subgenomes, designated D (Abdolmalaki et al., 2019), is related to the D genome of Ae. tauschii (2n = 2x = 14, DD; Kihara, 1949; Kimber and Zhao, 1983; Zhang and Dvořák, 1992; Badaeva et al., 1998, 2002; Edet et al., 2018), which also served as the cytoplasmic genome donor to this tetraploid species (Terachi et al., 1987; Kimber and Tsunewaki, 1988).”
The sentence has been corrected as follows:
“One of the Ae. crassa subgenomes, designated D1, is related to the D genome of Ae. tauschii (2n = 2x = 14, DD; Kihara, 1949; Kimber and Zhao, 1983; Zhang and Dvořák, 1992; Badaeva et al., 1998, 2002; Edet et al., 2018), which also served as the cytoplasmic genome donor to this tetraploid species (Terachi et al., 1987; Kimber and Tsunewaki, 1988).”
In Materials and methods, Fluorescence in situ hybridization, paragraph two, the sentence previously stated:
“At the same time, we have found earlier that hexaploid Ae. crassa, IG 131680 carries a translocation T1D (Abdolmalaki et al., 2019)L:7D (Abdolmalaki et al., 2019)L (T10) with interstitial breakpoints in addition to two species-specific translocations, T1 (Acr:6Xcr) and T2 (4D1S,FcrS; Badaeva et al., 2021).”
The sentence has been corrected as follows:
“At the same time, we have found earlier that hexaploid Ae. crassa, IG 131680 carries a translocation T1D1L:7D1L (T10) with interstitial breakpoints in addition to two species-specific translocations, T1 (Acr:6Xcr) and T2 (4D1S,FcrS; Badaeva et al., 2021).”
In Results, Fluorescence in situ hybridization, paragraph eight, the sentence previously stated:
“Small CL209-sites are present on chromosomes Ccr (middle of the short arm), 5Xcr (distal third of the long arm), and on a distal part of 7D (Abdolmalaki et al., 2019)L arm (Figure 1J; Supplementary Figures S3, S4).”
The sentence has been corrected as follows:
“Small CL209-sites are present on chromosomes Ccr (middle of the short arm), 5Xcr (distal third of the long arm), and on a distal part of 7D1L arm (Figure 1J; Supplementary Figures S3, S4).”
In Results, Fluorescence in situ hybridization, paragraph eight, the sentence previously stated:
“Hexaploid Ae. crassa has additional large subtelomeric CL209 clusters on chromosomes 1XcrS and in a distal part of 6D (Adams and Wendel, 2005)L (Figure 2F; Supplementary Figure S4).”
Figure 2.
Localization of: (A, C, J) CL27_232 (red) + o45 (green), (B) CL219 (red) + CL228(green); (D) CL232 (red) + pAs1 (green); (E) P332 (green); (F) CL261(red) + CL209 (green); (G, I) CL18 (red) + CL241 (green); (H) CL261 (red) + CL170 (green) on chromosomes of Ae. crassa, 4x (E), 6x (A, B, F, G), Ae. tauschii (C, D, H) and common wheat (I, J).
The sentence has been corrected as follows:
“Hexaploid Ae. crassa has additional large subtelomeric CL209 clusters on chromosomes 1XcrS and in a distal part of 6D2L (Figure 2F; Supplementary Figure S4).”
In Results, Fluorescence in situ hybridization, paragraph ten, the sentence previously stated:
“Hexaploid Ae. crassa has smaller signals of CL232 in a terminal part of 4D1S and distal quarters of 7D (Abdolmalaki et al., 2019)L and 6D (Adams and Wendel, 2005)L.”
The sentence has been corrected as follows:
“Hexaploid Ae. crassa has smaller signals of CL232 in a terminal part of 4D1S and distal quarters of 7D1L and 6D2L.”
In Results, Fluorescence in situ hybridization, paragraph eleven, the sentence previously stated:
“Among them, five sites are common (BcrL, CcrL, 5XcrL, 2D (Abdolmalaki et al., 2019)L, and 3D1), but tetraploid form contains two additional loci on 1D (Abdolmalaki et al., 2019)L and 2D1S.”
The sentence has been corrected as follows:
“Among them, five sites are common (BcrL, CcrL, 5XcrL, 2D1L, and 3D1), but tetraploid form contains two additional loci on 1D1L and 2D1S.”
In Results, Fluorescence in situ hybridization, paragraph fifteen, the sentence previously stated:
“Two clear signals are detected in the terminus and in the middle part of 1D1 and 6D1S short arms; a prominent, probably double signal is observed in the terminal part of 3DL (Abdolmalaki et al., 2019), and one or a pair of small signals are present in opposite arms of 2D1 and 7D1 (Figure 6).”
Figure 6.
Comparison of CL228 labeling patterns on chromosomes of different cereal species: TAU – Ae. tauschii ssp. strangulata; D1, 4x cr – D1 subgenome of tetraploid Ae. crassa; D1, 6x cr, D2, 6x cr – D1 and D2 subgenomes of hexaploid Ae. crassa; AEST – A, B, and D subgenomes of T. aestivum. 1–7 – homoeologous groups. White arrowheads show minor CL228 sites detected on the Xcr, A, B, and D subgenome chromosomes.
The sentence has been corrected as follows:
“Two clear signals are detected in the terminus and in the middle part of 1D1 and 6D1 short arms; a prominent, probably double signal is observed in the terminal part of 3D1L, and one or a pair of small signals are present in opposite arms of 2D1 and 7D1 (Figure 6).”
In Results, Fluorescence in situ hybridization, paragraph sixteen, the sentence previously stated:
“The largest signal occurs on 3DL (Adams and Wendel, 2005), similarly to 3DL (Abdolmalaki et al., 2019), and other intense sites are present in the proximal third of 2DL (Adams and Wendel, 2005) and a distal part of 7D2S.”
The sentence has been corrected as follows:
“The largest signal occurs on 3D2L, similarly to 3D1L, and other intense sites are present in the proximal third of 2D2L and a distal part of 7D2S.”
In Results, Fluorescence in situ hybridization, paragraph eighteen, the sentence previously stated “Most prominent sites appear on chromosome 2D1; subterminal signals on 3DL (Abdolmalaki et al., 2019) and 3DL (Adams and Wendel, 2005) are also large.”
The sentence has been corrected as follows:
“Most prominent sites appear on chromosome 2D1; subterminal signals on 3D1L and 3D2L are also large.”
In Discussion, Novel markers for chromosome and genome identification, paragraph seven, the sentence previously stated:
“Thus, three prominent CL131 clusters were detected on 2D1 and another one, overlapping with CL228, on 3DL (Abdolmalaki et al., 2019).”
The sentence has been corrected as follows:
“Thus, three prominent CL131 clusters were detected on 2D1 and another one, overlapping with CL228, on 3D1L.”
In Discussion, Evolutionary changes of repetitive DNA families in Aegilops crassa genome, paragraph five, the sentence previously stated:
“Genomic shock might cause massive amplification and spread of the repeat to other chromosomal sites, leading to the emergence of prominent CL219 clusters in proximal (7D1S) and distal 7DL (Abdolmalaki et al., 2019) chromosome regions.”
The sentence has been corrected as follows:
“Genomic shock might cause massive amplification and spread of the repeat to other chromosomal sites, leading to the emergence of prominent CL219 clusters in proximal (7D1S) and distal (7D1L) chromosome regions.”
In the Introduction, paragraph two, a sentence previously stated:
“The D subgenome is also present in two polyploid Aegilops species and in four exaploidy bread wheat Triticum aestivum (2n = 6x = BBAADD).”
The sentence has been corrected as follows:
“The D subgenome is also present in two polyploid Aegilops species and in hexaploid bread wheat Triticum aestivum (2n = 6x = BBAADD).”
In the Introduction, paragraph two, a sentence previously stated:
“According to molecular analysis of nuclear genome (Dvořák et al., 1998; Luo et al., 2017; Singh et al., 2019) and hybridization pattern of pAs1 probe (Badaeva et al., 1996, 2019a; Zhao et al., 2018; Ebrahimzadegan et al., 2021), the D subgenome of polyploid wheat was inherited from Ae. tauschii subsp. four exaploidy, while Ae. tauschii subsp. tauschii contributed the D subgenome to polyploid Aegilops species Ae. cylindrica and 6x Ae. crassa (Badaeva et al., 2002).”
The sentence has been corrected as follows:
“According to molecular analysis of nuclear genome (Dvořák et al., 1998; Luo et al., 2017; Singh et al., 2019) and hybridization pattern of pAs1 probe (Badaeva et al., 1996, 2019a; Zhao et al., 2018; Ebrahimzadegan et al., 2021), the D subgenome of polyploid wheat was inherited from Ae. tauschii subsp. strangulata, while Ae. tauschii subsp. tauschii contributed the D subgenome to polyploid Aegilops species Ae. cylindrica and 6x Ae. crassa (Badaeva et al., 2002).”
In the Introduction, paragraph three, a sentence previously stated:
“Kihara (1963) proposed that this subgenome could be inherited from Ae. comosa and suggested genomic formula DM for tetraploid and DDM for four hexaploid Ae. crassa.”
The sentence has been corrected as follows:
“Kihara (1963) proposed that this subgenome could be inherited from Ae. comosa and suggested genomic formula DM for tetraploid and DDM for hexaploid Ae. crassa.”
In Materials and methods, Real-time quantitative PCR, paragraph one, a sentence previously stated:
“Primers 2) were synthesized at Syntol Ltd. (Moscow, Russia).”
The sentence has been corrected as follows:
“Primers were synthesized at Syntol Ltd. (Moscow, Russia).”
In Materials and methods, DNA probes for FISH, paragraph one, a sentence previously stated:
“The following novel repeats identified by means of low-coverage sequencing followed by bioinformatics analysis were used as FISH probes: (i) derived from 4× Ae. crassa genome: CL3, CL8 (highly homologous to CL16 found in Ae. Tauschii subsp. nine trangulate), CL18, CL60, CL131 (highly homologous to CL149 found in Th. bessarabicum), CL170, CL193, CL209, CL219, CL228, CL232, CL239, CL241, CL244, CL257, CL258, CL261 (highly homologous to CL198 found in Th. bessarabicum); ii) derived from 6x Ae. crassa genome: CL27_232; iii) derived from Th. bessarabicum genome: CL2, CL148.”
The sentence has been corrected as follows:
“The following novel repeats identified by means of low-coverage sequencing followed by bioinformatics analysis were used as FISH probes: (i) derived from 4× Ae. crassa genome: CL3, CL8 (highly homologous to CL16 found in Ae. tauschii subsp. strangulata), CL18, CL60, CL131 (highly homologous to CL149 found in Th. bessarabicum), CL170, CL193, CL209, CL219, CL228, CL232, CL239, CL241, CL244, CL257, CL258, CL261 (highly homologous to CL198 found in Th. bessarabicum); ii) derived from 6x Ae. crassa genome: CL27_232; iii) derived from Th. bessarabicum genome: CL2, CL148.”
In Materials and methods, Fluorescence in situ hybridization, paragraph one, a sentence previously stated:
“The A subgenome chromosomes of wheat were classified using additional probe combination GAAn and pTa-535 according to Komuro et al. (2013); chromosomes of Ae. Tauschii were classified as suggested by Badaeva et al. (2002, 2019a) and Zhao et al. (2018), Ae. crassa as in Abdolmalaki et al. (2019), and Badaeva et al. (2021).”
The sentence has been corrected as follows:
“The A subgenome chromosomes of wheat were classified using additional probe combination GAAn and pTa-535 according to Komuro et al. (2013); chromosomes of Ae. tauschii were classified as suggested by Badaeva et al. (2002, 2019a) and Zhao et al. (2018), Ae. crassa as in Abdolmalaki et al. (2019), and Badaeva et al. (2021).”
In Materials and methods, Fluorescence in situ hybridization, paragraph two, the sentence previously stated:
“Our previous analyses showed that accessions K-2485 and AE 742 (Ae. crassa, 4x), K-112 (Ae. tauschii subsp. strangulata), PI 201890 (Th. bessarabicum), and Chinese Spring have normal karyotypes typical to the respective species (Gill et al., 1991; Badaeva et al., 2019a, 2019b, 2021).”
The sentence has been corrected as follows:
“Our previous analyses showed that accessions K-2485 and AE 742 (Ae. crassa, 4x), K-112 (Ae. tauschii subsp. strangulata), PI 531711 (Th. bessarabicum), and Chinese Spring have normal karyotypes typical to the respective species (Gill et al., 1991; Badaeva et al., 2019a, 2019b, 2021).”
In Results, Repeats’ characterization, paragraph two, point one previously stated: “AC4x_CL3_339nt was more dissimilar to P335 repeat from Th. besarabicum genome.”
This has been corrected as follows:
“AC4x_CL3_339nt was more dissimilar to P335 repeat from Ae. tauschii genome.”
In Results, Fluorescence in situ hybridization, paragraph seventeen, a sentence previously stated:
“Several very weak but consistent signals appear on 1B, 4B, 5B, 1A, 3A, 4A, and 6A chromosomes (Figure 6).”
The sentence has been corrected as follows:
“Several very weak but consistent signals appear on 2B, 4B, 5B, 1A, 3A, 4A, and 6A chromosomes (Figure 6).”
In Results, Fluorescence in situ hybridization, paragraph twenty-one, a sentence previously stated:
“For all these repeats heteromorphisms of homologous chromosomes in signal presence and/ or size is often observed.”
The sentence has been corrected as follows:
“For all these repeats heteromorphisms of homologous chromosomes in signal presence and/ or size is often observed.”
In Discussion, Novel markers for chromosome and genome identification, paragraph nine, a sentence previously stated:
“According to bioinformatics and qPCR the novel CL239 repeat is absent in Ae. tauschii (sub)genomes.”
The sentence has been corrected as follows:
“According to bioinformatics and qPCR the novel CL239 repeat is absent in the Ae. tauschii genome.”
In Discussion, Evolutionary changes of repetitive DNA families in Aegilops crassa genome, paragraph eight, a sentence previously stated:
“We can reach a similar conclusion considering that the CL170 repeat is absent from Xcr genome, but is abundant in the D (sub)genome of wheat and Ae. tauschii, Ae. crassa, and Th. bessarabicum.”
The sentence has been corrected as follows:
“We can reach a similar conclusion considering that the CL170 repeat is absent in Xcr genome, but is abundant in the D (sub)genome of wheat and Ae. tauschii, Ae. crassa, and Th. bessarabicum.”
The publisher apologizes for these errors. The original article has been updated.
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