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. 2023 Jul 15;12(14):1863. doi: 10.3390/cells12141863

Figure 1.

Figure 1

Policosanol stimulates osteoblast differentiation. (a) MC3T3-E1 cells were treated with 50 μg/mL of policosanol for 4 days and the expression of mRNA encoding Dlx5 and Runx2 was measured using qPCR. (b) Western blotting was performed after treatment of MC3T3-E1 cells with 50 μg/mL for 8 days. (c) ALP staining was performed after treatment of cells with ascorbic acid (A.A), β-glycerophosphate (β-GP), and 50 μg/mL policosanol for 8 days. (d) Extracellular mineralization was determined using ARS staining. Cells were treated with A.A, β-GP, and 50 μg/mL policosanol for 21 days. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the untreated control, ## p < 0.01 compared with the A.A and β-GP-treated group. Data represent the mean ± SD of three individual experiments.