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. 2023 Jul 15;12(14):1863. doi: 10.3390/cells12141863

Figure 4.

Figure 4

INSIG inhibition decreases policosanol-induced osteoblast differentiation. MC3T3-E1 cells were transfected with 100 nM INSIG1 or -2 siRNA. (a) INSIG1 and Runx2 mRNA expression were determined after transfection and then treatment with policosanol for 4 days. (b) mRNA expression of INSIG2 and Runx2 was measured using qPCR. (c,d) Total protein was extracted after the treatment of policosanol for 4 days. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the untreated control. # p < 0.05, ## p < 0.01 compared with the policosanol-treated group. Data represent the mean ± SD of three individual experiments.