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. 2023 Jul 20;11(7):1842. doi: 10.3390/microorganisms11071842

Table 2.

qRT-PCR primer sequences used in this work.

Gene Function Primer Temperature Annealing (°C) Reference
lchAA Lichenysin synthetase F: 5′-ACTGAAGCGATTCGCAAGTT-3′ 56 [30]
R: 5′–TCGCTTCATATTGTGCGTTC-3′
srfA Surfactine synthetase F:5′- ATGAGCCAACTCTTCAAATCATTTGAT-3′ 52 [48]
R: 5′-TCACGGTTGAATGATCGGATGCTGATT-3′
tufGP Elongation factor-Tu (housekeeping gene) F: 5′-ACGTTGACTGCCCAGGACAC-3′ 55 [49]
R: 5′GATACCAGTTACGTCAGTTGTACGGA-3′