Chemical Constituents and Their Biological Activities from Genus Styrax

Abstract

Plants from the genus Styrax have been extensively used in folk medicines to treat diseases such as skin diseases and peptic ulcers and as an antiseptic and analgesic. Most Styrax species, especially Styrax tonkinensis, which is used as an expectorant, antiseptic, and analgesic in Chinese traditional medicine, could screen resin after external injury. Styrax is also used in folk medicines in Korea to treat sore throat, bronchitis, cough, expectoration, paralysis, laryngitis, and inflammation. Different parts of various Styrax species can be widely employed for ethnopharmacological applications. Moreover, for ethnopharmacological use, these parts of Styrax species can be applied in combination with other folk medicines. Styrax species consist of versatile natural compounds, with some of them exhibiting particularly excellent pharmacological activities, such as cytotoxic, acetylcholinesterase inhibitory, antioxidant, and antifungal activities. Altogether, these exciting results indicate that a comprehensive review of plants belonging to this genus is essential for helping researchers to continuously conduct an in-depth investigation. In this review, the traditional uses, phytochemistry, corresponding pharmacological activities, and structure–activity relationships of different Styrax species are clarified and critically discussed. More insights into potential opportunities for future research are carefully assessed.

1. Introduction

The genus Styrax has a widespread but dispersive distribution. It is found in East Asian, American, and Mediterranean regions. It is the largest genus of the Styracaceae family and contains approximately 130 species [1]. Styrax stands out from other genera in this family because it produces a resinous material known as benzoin resin. This resin is typically released when the bark is injured by sharp objects. It has been utilized in various regions across the globe for its aromatic properties, being commonly used in perfumes and cosmetics, and Styrax species have traditionally been used in herbal medicines for the treatment of various diseases [2]. Of note, many Styrax species, especially S. tonkinensis, which is used as an expectorant, antiseptic, and analgesic in Chinese traditional medicine, could screen resin after exterior injury [3,4]. As a folk medicine in Korea, S. japonica is used to treat cough, bronchitis, sore throat, inflammation, paralysis, laryngitis, and expectoration [5,6,7]. The resin from Styrax, mixed with other antibiotic substances and hardening material, is also indicated in Islamic medicine as working as a good dental restorative material [2]. The flower of S. japonicus sieb. et Zucc. is used in Chinese folk medicine to relieve pain such as sore throat pain and toothache [8]. The leaves and roots of Styrax suberifolium are typically used as traditional medicines in China to cure rheumatic diseases [9].

Furthermore, the extensive investigation of pharmacologically active compounds derived from various Styrax species has been ongoing for several decades. While numerous Styrax species have been studied, S. obassia and S. japonica have emerged as the most extensively researched species, encompassing studies ranging from phytochemistry to comprehensive pharmacological investigations (Figure 1). An example of the pharmacological potential of Styrax species was the inhibitory effect of benzofurans extracted from Styrax agrestis A. Chev. on acetylcholinesterase (AChE) in vitro [10]. Triterpenoids isolated from the resin of S. tonkinensis (Pier.) Craib showed promising antiproliferative and differentiation effects on human leukemia HL-60 cells [4]. Additionally, the hydroalcoholic extracts of S. camporum Pohl demonstrated effectiveness in reducing chromosome and DNA damage [11]. Another notable finding was the promotion of estrogen biosynthesis by egonol gentiobioside and egonol gentiotrioside from Styrax perkinsiae through the action of aromatase [12].

Figure 1.

Research overview of the Styrax genus.

Despite significant progress in discovering natural compounds from Styrax species and elucidating their potential pharmacological activities, there is still a need for a comprehensive and focused discussion of this rapidly growing research area. With our continuous interests in natural products discovery and pharmacological research [13,14,15,16], our aim is to provide researchers with a convenient and comprehensive resource that offers detailed and concise profiles of the Styrax genus. This review encompasses the examination of structural diversity and the pharmacological and biological significance and presents the exciting future research prospects in this field.

2. Results and Discussion

2.1. Chemical Constituents

2.1.1. Lignans

Lignans are the major constituents isolated from Styrax species. Most lignans are benzofuran, tetrahydrofuran, and furofuran lignans, and they are found in the stem nucleus of S. perkinsiae, S. ferrugineus, S. macranthus, S. obassia, S. camporun, S. japonica, and S. officinalis L. [2]. S. perkinsiae contains 16 norlignans including 1–14 and lignans 15 and 16 [17,18]. Norlignans (2, 4, and 17–19) from S. ferrugineus leaves were investigated and characterized [19]. Compounds 5 and 20–35 were isolated from the stem bark of S. japonica by several research groups [7,20,21,22,23,24]. Meanwhile, lignans 4, 15, and 16 were also afforded from S. japonica seeds [25]. Constituents of S. obassia were investigated, and norlignans (2, 3, 6, and 36) were isolated [26]. Moreover, a series of reports revealed the presence of several benzofurans in S. obassia including 37–42 [27,28,29,30,31]. Six benzofuran derivatives comprising 4–7, 14, and 43–46 were afforded from the seeds of S. macranthus that grow in southwestern China [32,33]. Benzofurans 4, 15, 40, and 47–49 were isolated from the hexane extract of the seeds of S. officinalis L. [34,35,36,37]. Thirteen compounds, 4, 15, 37, 39–42, and 50–55, were obtained from the ethyl acetate (EtOAc) extract of the fruits of S. agrestis [10]. Moreover, compounds 4, 17, and 56 were isolated from S. camporum, and their protective activities were continuously assessed in vivo [11,38]. Bertanha et al. isolated benzofuran nor-neolignan derivatives 4, 6, 17, 18, and 57 from the aerial parts of S. pohlii. Several lignans including 58–65 were isolated from S. perkinsiae [39]. Seventeen phenylpropanoids were successfully isolated from the bark of S. suberifolius, including ten benzofuran derivatives (4–5, and 66–73), two dihydrofuran derivatives (23 and 65), two new neolignans (74 and 75), and three benzalcohols (76–78) [40]. Eight lignans (79–85) were isolated from the leaves of S. tonkinensis (Pierre) Craib ex Hartw [41]. Two new phenylpropanoids (86 and 87) were isolated from the resin of S. tonkinensis (Pierre) Craib ex Hartw by Fang’s groups [42]. Two lignans (88 and 89) and five nor-lignan-type benzofurans, including 4 and 90–93, were separated from S. argentifolius by Son’s group [43].

2.1.2. Terpenoids

Terpenoids were also obtained from the Styrax genus as one of its major constituents. It should be noted that a vast majority of the terpenoids isolated from the Styrax genus were pentacyclic triterpenoids. To date, these molecules were only found in four species of the Styrax genus. Compounds 94–102 were isolated from the stem bark of S. japonica Sieb. et Zucc. by several research groups [20,22,44,45]. A phytochemical investigation on the fresh fruits of S. japonica Sieb. et Zucc. was also conducted, and four new triterpenoid glycosides including jegosaponins A–D (103–106) were found [46]. Furthermore, S. japonica Sieb. et Zucc. continued to be investigated by Kwon’s group, and 107–110 and taraxerol (94) were isolated [47]. In addition to the plants themselves, triterpenoids were also found from the resin of S. tonkinensis (Pier.) Craib containing 111–119 [4,6]. A pentacyclic triterpenoid (120), three triterpenoid saponins styrax-saponins A-C (121–123), and deacylsaponin (124) were also obtained from S. officinalis L. [48,49]. Moreover, several monoterpenes, such as α-terpineol, linalool, and geraniol, were isolated from the benzoe resin of S. officinalis L. [50]. Recently, two cinnamyl esters and seven pentacyclic triterpene acids (119, 125–130) were separated and characterized from S. tonkinensis (Pierre) Craib ex Hartw [42,51]. A triterpenoid (131) was obtained from S. argentifolius very recently [43].

2.1.3. Aromatic Compounds

Aromatic compounds, as a small proportion, were reported in the Styrax genus as well. In the species of S. tonkinensis (Pier.) Craib, seven aromatic compounds including 132–139 were reported [52,53]. Moreover, Kim and coworkers found 140 and 141 from the stem bark of S. japonica (SJ) [54]. In S. perkinsiae Rhed., 142 was separated [39]. Recently, a new epicatechin glucopyranoside, 143, and three mononuclear phenolic acid esters, 144–146, were isolated from the bark of S. suberifolius Hook [40].

2.1.4. Steroids

Luo and coworkers reported that three steroids including stigmasterol (147), styraxosides A (148), and daucosterol (149) were obtained from the seeds of S. macranthus Perk [32]. Another Steroid named β-sitosterol (150) was reported in S. perkinsiae Rehder [17]. A sterol, 151, was separated from S. argentifolius H.L. Li by Son’s group [43].

2.1.5. Others

In addition to the commonly isolated products from the genus Styrax, other types of natural products were also reported with relatively limited numbers. For example, in 1973, a preliminary result regarding the seeds of S. officinalis L. showed that the oil content amounts to 50% [55]. Moreover, flavonoids are not frequently reported in the Styrax genus according to literature studies. Only four flavonoids including 152–155 were isolated from the aerial parts of S. pohlii A. DC. and the leaves of S. camporum Pohl [56]. Later, two new polyketones, 156–157, were isolated from Styrax camporum Pohl. [57]. Recently, two bioactive saponins, Jegosaponin A and B (158–159), were extracted and subsequently identified from S. japonica Siebold et al. Zuccarini [58].

2.2. Chemical Constituents Biological Activities

2.2.1. Cytotoxic Activity

S. perkinsiae was investigated, and the cytotoxic activity of the compounds isolated from this species was tested through the colorimetric chemosensitivity assay with SRB. (Figure 2). Interestingly, 11 and 14 revealed cytotoxic activities in vitro against two breast cancer cell lines, MCF-7 (IC₅₀ = 5.5 and 15.0 µg/mL, respectively) and MDA-MB-231 (IC₅₀ = 3.81 and 13.71 µg/mL, respectively) [17].

Figure 2.

Natural compounds with cytotoxic activity.

Later, the cytotoxic activities of lignans isolated from S. camporum against three cell lines, namely, HeLa (human cervix carcinoma), C6 (rat glioma), and Hep-2 (larynx epidermoid carcinoma), were analyzed using the standard MTT. Compound 4 showed strong cytotoxic activities against the Hep-2 (IC₅₀ = 3.6 µg/mL) and C6 (IC₅₀ = 3.2 µg/mL) cell lines. Compound 17 exhibited significant cytotoxic activities against the HeLa (IC₅₀ = 5.3 µg/mL) and C6 (IC₅₀ = 4.9 µg/mL) cell lines. Compound 56 exhibited moderate cytotoxic activities against the Hep-2 (IC₅₀ = 28.0 µg/mL), HeLa (IC₅₀ = 31.7 µg/mL), and C6 (IC₅₀ = 10.7 µg/mL) cell lines. Moreover, when combined, 4 and 17 exhibited higher cytotoxic activities than the hydroalcoholic extract or either of the lignans alone, with the lowest IC₅₀ being 13.3 µg/mL [38,59].

Seven compounds isolated from S. obassia were screened for their cytotoxic activities against the HeLa, HL-60, and MCF-7 cell lines. Among them, compounds 3 and 5 exhibited significant antitumor properties. Compound 3 exhibited cytotoxicity against the HeLa (IC₅₀ = 23.3 µg/mL), HL-60 (IC₅₀ = 16.8 µg/mL), and MCF-7 cells (IC₅₀ = 53.5 µg/mL). Meanwhile, compound 5 exhibited cytotoxicity against HeLa (IC₅₀ = 23.3 µg/mL), HL-60 (IC₅₀ = 47.8 µg/mL), and MCF-7 cells (IC₅₀ = 27.9 µg/mL) [60].

Through the Cell Counting Kit-8 (CCK-8) test in vitro, compounds 86 and 87 were tested for their cytotoxic activities against five tumor cell lines (PC-3, MCF-7, A549, HeLa, and HepG-2). Among them, the cytotoxic effect of compound 86 was observed against the MCF-7 and HeLa cell lines (IC₅₀ = 26.75 and 45.16 µM, respectively), which was better or similar to that of the positive control cisplatin (IC₅₀ = 40.95 and 47.36 μM, respectively). Compound 86 exhibited moderate cytotoxicity against the PC-3 and HepG-2 cell lines. The other biomolecule, 87, displayed moderate cytotoxicity against MCF-7 cells (IC₅₀ = 57.1 µM) [42].

Son’s group assessed the cytotoxicity and α-glucosidase inhibitory activity of isolated compounds from S. argentifolius. They suggested that the activities of triterpenoid 131 and norlignan-type benzofurans (4 and 91–93) are superior to those of others including sterol 153 and lignans 88 and 89. The better activities of benzofurans (4 and 91–93) were postulated to be an effect of the substitutions at the side chain of carbon C-5. Among them, compound 4 exhibited potential cytotoxicity against three cancer cell lines, namely, Lu (IC₅₀ = 21.50 µg/mL), KB (IC₅₀ = 22.11 µg/mL), and HepG-2 (IC₅₀ = 18.15 µg/mL) [43].

2.2.2. Antibacterial and Antifungal Activity

Initially, the extract of S. ferrugineus exhibited antifungal and antibacterial activities against Candida albicans, Cladosprorium sphaerospermum, and Staphylococcus aureus. To identify the potential biomolecules from this species that exhibit antifungal and antibacterial activities, the isolated lignans were tested (Figure 3). Among them, lignans 4 and 17 exhibited antifungal and antibacterial activities against S. aureus (MIC = 10 μg/mL and 20 μg/mL, respectively), C. albicans (MIC = 10 μg/mL and 12 μg/mL, respectively), and C. sphaerospermum (MIC = 5 μg/mL and 10 μg/mL, respectively), whereas the other three natural products (5, 18, and 19) only inhibited C. albicans (MIC = 15 μg/mL, 20 μg/mL, and 15 μg/mL, respectively) and S. aureus (MIC = 20 μg/mL, 20 μg/mL, and 20 μg/mL, respectively) [19].

Figure 3.

Natural compounds with antibacterial and antifungal activities.

To exploit the antibacterial activity of the aerial parts of S. pohlii, different fractions, especially those extracted using n-hexane, EtOAc, n-BuOH, and methanol, were evaluated against Haemophilus influenzae, Pseudomonas aeruginosa, S. pyogenes, Streptococcus pneumoniae, and Klebsiella pneumoniae. The broth microdilution method was used for measuring the minimum inhibitory concentration (MIC). Among the fractions, the n-hexane fraction exhibited excellent antibacterial activity against Gram-positive S. pneumoniae (MIC = 200 μg/mL). The MIC values of compounds 4 and 17 (400.0 µg/mL) against P. aeruginosa and S. pneumoniae were the best [61].

By conducting the radial growth-inhibition experiment, the antifungal activities of compounds from the bark of S. suberifolius against three plants’ fungal pathogen, namely, Phomopsis cytospore, Fusarium oxysporum, and Alternaria Solani, was exhibited. Compounds 144, 145, and 146 exhibited selective suppressive activities against the tested fungi. Notably, compound 146 was a significantly effective inhibitor of Phomopsis cytospore at 100.0 µg/mL, with an inhibition rate of 86.72% [40].

2.2.3. Antiproliferative and Differentiation Effects

In 2006, Wang’s group found that triterpenoids (111–120) isolated from S. tonkinensis inhibit HL-60 cell growth (IC₅₀ = 8.9–99.4 µM). Of note, oleanolic acid 119 acted as the most effective antiproliferative agent (IC₅₀ = 8.9 µg/mL) (Figure 4). Compound 113 exhibited the lowest growth-inhibitory effect. According to the NBT-reduction assay, compound 113 induced HL-60 cell differentiation, as measured in [4].

Figure 4.

Natural compounds with antiproliferative and differentiation properties.

2.2.4. Anti-Complement Activity

Egonol (4), masutakeside I (10), styraxlignolide A (28), and styraxoside B (101) isolated from S. japonica could inhibit the hemolytic activity of the complement system (IC₅₀ = 33, 166, 123, and 65 µM, respectively) (Figure 5). This finding strongly suggested that the methyl enedioxy group of lignans has a vital role in inhibiting the hemolytic activity of human serum against erythrocytes [22].

Figure 5.

Natural compounds with anti-complement activity.

2.2.5. Anti-Complement Activity

Natural products isolated from S. japonica were tested for in vitro antioxidant activities through the DPPH radical scavenging test. Among them, 30–33 exhibited weak DPPH radical scavenging activities (IC₅₀ = 380, 278, 194, and 260 µM, respectively) (Figure 6) [7]. Moreover, Oliveira et al. reported that the hydroalcoholic extract of S. camporum could concentration-dependently scavenge DPPH radicals; a maximum scavenging activity of 85% was observed at 30.0 µg/mL [11].

Figure 6.

Natural compounds with antioxidant activity.

2.2.6. Induction of Apoptosis

Lee and Lim revealed that the ethanol extract of S. japonica Siebold et al., Zuccarini (SJSZ) induced programmed cell death (apoptosis) in HepG2 cells under the experimental condition (75.0 µg/mL of SJSZ for 4 h treatment). The results indicated that the ethanol extract of SJSZ (75 µg/mL) stimulates an increase in the number of iROS, Ca²⁺, and the apoptotic-related factors in HepG2 cells [62].

2.2.7. Induction of Apoptosis

In 2002, a nonradioactive assay was established for measuring aromatase activity by using human ovarian granulosa KGN cells. Lignans 6 and 7 exhibited approximately 1.62- and 1.95-fold increases, respectively, in 17 β-estradiol biosynthesis at 10 µM, and significantly improved 17 β-estradiol biosynthesis by approximately 1.53- and 1.71-fold, respectively, in 3T3-L1 preadipocyte cells (Figure 7). Moreover, egonol gentiotrioside increased serum estrogen levels in ovariectomized rats. These results suggested that these two lignans induce estrogen biosynthesis through the allosteric regulation of aromatase activity [12].

Figure 7.

Natural compounds exhibiting estrogen-promoting activity.

2.2.8. Acetylcholinesterase Inhibitors and Structure–Activity Relationships

In 2011, Liu et al. screened their library of plant extracts through a high-throughput assay. They found that the EtOAc extract of S. agrestis fruits exhibited significant inhibitory activity against AChE. They proved that two active subfractions were responsible for this inhibition and further isolated 13 compounds from the EtOAc extract. Later, they examined the selectivity and inhibitory potency of benzofurans on hAChE, BChE, and EeAChE by using the improved Ellman’s colorimetric method (Figure 8). Some egonol derivatives were synthesized through chemical modifications to clearly understand the structure–activity relationships. According to the results, the inhibition ratio affects the bulkiness and length of the alkyl ester group. In particular, compounds 50–53 exhibited inhibitory activity against AChE (IC₅₀ = 1.4–3.1 μM). Compound 50 at 100.0 μM displayed obvious inhibition of Aβ aggregation (77.6%). Liu et al.’s SAR (Structure-Activity Relationships) studies indicated that compounds exhibiting anti-AChE activity are observed with the incorporation of alkyl chains consisting of more than three carbon units, the furan ring, and the ester group. Molecular docking studies proposed a binding site for this class of compound on AChE and identified multiple key residues at the peripheral site that are crucial for mediating the inhibitory effect [10]. The anti-AChE and antifungal activities of two novel polyketides, 156 and 157, were also tested through TLC bioautographic assays. The results indicated that compound 156 could inhibit AChE activity [57].

Figure 8.

Natural compounds as potential acetylcholinesterase inhibitors.

2.2.9. Inhibitory Effect on Interleukin

Lee and Lim separated a glycoprotein with an approximate molecular mass of 38 kDa from S. japonica. Subsequently, an immunoblot analysis and RT-PCR were conducted to evaluate ERK, JNK, and NF-κB activities and the levels of inflammation-related factors (COX-2, inducible nitric oxide synthase (iNOS), and interleukin (IL)-1β) in Cr-induced BNL CL.2 cells. The SJSZ glycoprotein (50.0 µg/mL) inhibited the expression of ERK, NF-κB, JNK, iNOS, IL-1β, and COX-2 [63].

With further investigation of the SJSZ glycoprotein (38 kDa), Lee and Kim proved that this glycoprotein modulates IFN-γ, IL-2, and IL-12 expression in cyclophosphamide (CTX)-induced Balb/c mice. The glycoprotein counteracted the CTX-induced immunosuppressive effects. It effectively restored the spleen and thymus weights to normal levels and enhanced the phagocytic activity of peritoneal macrophages in response to CTX. Furthermore, the SJSZ glycoprotein exerted regulatory effects on the proliferation of T and B lymphocytes, cytotoxicity of NK cells, and production of key cytokines (IIFN-γ, L-2, and IL-12). Additionally, it improved the activity of antioxidant enzymes (e.g., SOD, CAT, and GPx) [64].

2.2.10. Matrix Metalloproteinase’s Activity

Some triterpenoids (95–97, 100) were isolated and further tested the Matrix Metalloproteinases (MMPs)’ activity of the methylene chloride soluble fraction of a methanol extract from the stems of S. japonica. Among them, 95 and 100 displayed effective cytotoxic activities against human dermal fibroblasts (IC₅₀ = 20.0 and 1.12 µM, respectively) (Figure 9). In addition, 96 and 97 exhibited no cytotoxicity for the same cells at the test dose (0.01–1 µM). However, 96 dose-dependently reduced UV-induced MMP-1 protein levels to normal levels by 73.1% at 0.01 µM [65]. In a dose-dependent manner, 96 effectively downregulated MMP-1 protein expression, whereas it upregulated type-1 procollagen protein expression in the UV-irradiated cultured human skin fibroblasts of an elderly person [66].

Figure 9.

Natural compounds with matrix metalloproteinase’s activity.

Styrax japonoside B (26) exerted inhibitory activity against MMP-1 and prevented UV-induced changes in MMP-1 expression. At 10 µM, the treatment led to a significant dose-dependent reduction in MMP-1 protein expression, with an average decrease of 62.1% compared with the vehicle-treated control cells. The findings suggested that the glycoprotein can potentially be used as a potent antimetastatic agent. This glycoprotein exerts its effect by suppressing MMP-9 enzymatic activity through the NF-κB and AP-1 signaling pathways [67].

Two cinnamyl esters (86 and 87) and seven pentacyclic triterpene acids (119 and 125–130) in Styrax are the key components that inhibit hCES1A activity. These seven pentacyclic triterpene acids in the two active sites of Styrax exert a significant inhibitory effect on hCES1A (IC₅₀ = 41–478 nM). Among them, epibetulinic acid (129) (IC₅₀ = 0.041 µg/mL), oleanonic acid (125) (IC₅₀ = 0.49 µg/mL), and betulonic acid (126) (IC₅₀ = 1.48 µg/mL) exhibited the strongest inhibitory activity against hCES1A [42,51].

2.2.11. Antiasthmatic, Antiulcer, and Anti-Inflammatory Activities

In a murine asthma model, homoegonol (17) exerted significant effects in reducing inflammatory cell infiltration and Th2 cytokine production in the bronchoalveolar lavage fluid. It also attenuated airway hyperresponsiveness, decreased serum IgE levels, and downregulated iNOS and MMP-9 expression. Thus, compound 17 exhibited the potential to effectively suppress OVA challenge-induced asthmatic responses (Figure 10).

Figure 10.

Natural compounds with antiasthmatic, antiulcer, and anti-inflammatory activities.The EtOAc fraction of S. camporum displayed antiulcer activity. It reduced the ulcer area and gastric secretion volume and increased the number of collagen fibers [37,68].

In 2005, the extracted fractions of S. pohlii aerial parts, including the EtOAc fraction, ethanolic extract, and hexane fraction, were evaluated for their inhibitory activities against COX-1 and COX-2. The isolated products were further assessed against COX-1 and COX-2. The results revealed that all crude fractions and isolated products induced weak-to-moderate COX-1 and COX-2 inhibition. Among them, 57 exerted mild COX-1 inhibition, of 35.7% at 30 µM [69].

2.2.12. Other Activity

Through micronucleus and comet assays, Oliveira demonstrated that different doses (250, 500, and 1000 mg/kg body weight) of the S. camporum extract’s compounds 4 and 17 had no genotoxic effect in Swiss mice. Moreover, they were effective in reducing doxorubicin- and methanesulfonate-induced DNA and chromosomal damage [11].

Braguine [69] investigated the EtOAc fractions of S. camporum and S. pohlii and isolated and identified compounds 152–155. Upon biological evaluation, they found that the EtOAc fractions, as well as compounds 152 and 155, could separate coupled Schistosoma mansoni adult worms. Additionally, compound 155 killed adult schistosomes in vitro. This research group also observed that homoegonol and homoegonol glucoside exhibited the best results against S. mansoni adult worms [70].

In vitro assessments were conducted to determine the protein tyrosine phosphatase 1B (PTP1B)’s inhibitory activities of compounds from S. japonica stem bark. Among the isolated compounds, 108 and 109 had the highest inhibitory activities (IC₅₀ = 7.8 and 9.3 μM, respectively) [45].

By downregulating NF-κB–DNA binding activity, styraxoside A (148) derived from S. japonica exerted inhibitory effects on the expression of LPS-induced iNOS, COX-2, tumor necrosis factor-α, and IL-1β [45].

Jegosaponins A and B (158 and 159, respectively) exhibited potent hemolytic activity in sheep defibrillation (IC₅₀ = 2.1 and 20.2 µg/mL, respectively) and could improve the performance of PC-3 cells and zebrafish embryos through the identification of a membrane nonpermeable DRAQ7, which is a fluorescent nucleus staining dye [58] (Figure 11).

Figure 11.

Natural compounds with other properties.

3. Materials and Methods

Through the search of a variety of online libraries such as Wiley Online Library, PubMed, Scifinder Web, ACS, and Web of Science, a summary of the newly discovered chemicals isolated from the genus styrax and their related biological activities in recent decades was provided. All species names were checked using http://www.theplantlist.org (accessed on 10 May 2023).

4. Conclusions

In summary, the Styrax genus comprises 130 species, and most of the species are extensively used as traditional medicines (Appendix A), particularly in China and Korea. Styrax can be easily collected because of its extensive distribution. All the species of the Styrax family, which were reported regarding the aspects of phytochemistry and pharmacology, were comprehensively summarized. In total, 159 compounds (Appendix B), including lignans, terpenoids, steroids, etc., were isolated from various species. The biological activities of those isolated compounds were subsequently investigated, exhibiting broad bioactivities such as cytotoxic activity, antioxidant activity, antifungal activity, apoptotic activity, anti-inflammation activity, anti-complement activity and so on. Chemical and pharmacological studies on the Styrax genus also proved that its main constituents are lignans and terpenoids. Moreover, several bioactive molecules exhibiting strong pharmacological activities were also isolated from Styrax (Appendix C).

Of note, information about the structure–activity relationships of most bioactive compounds is insufficient due to the lack of derivatives. Therefore, the exploitation of the versatility of the potentially bioactive natural compounds obtained from this genus is in great demand. Moreover, some species used in traditional medicines are still untapped such as S. suberifolius, which is used as a cure for rheumatic arthritis, whereas the modern physiochemical and pharmacological investigations are missing. Furthermore, in-depth pharmacological studies, especially in vivo studies, of the isolated biomolecules should be conducted in the future.

Abbreviations

MCF-7	Metastatic breast adenocarcinoma cell line
MDA-MB-231	Human breast cancer cell line
Hep-2	Human larynx carcinoma cell line
HeLa	Human cervix carcinoma cell lines
C6	Rat glioma cell lines
HL-60	Human leukemia cell line
A549	Human lung cancer cell line
PC-3	Human prostatic cancer cell line
Lu	Lung cancer cell lines
CCK-8	Cell Counting Kit-8
ATCC 6305	Streptococcus pneumoniae
ATCC 19615	Streptococcus pyogenes
ATCC 10211	Hemophilus influenzae
ATCC 27853	Pseudomonas aeruginosa
ATCC 10031	Klebsiella pneumoniae
MIC	Minimum inhibitory concentration
DPPH	DPPH radical
HepG2	Hepatocellular carcinoma cell line
KGN	Human ovarian granulosa cells
AChE	Acetylcholinesterase
EeAChE	Electrophorus electricus AChE
hAChE	Human AChE
BChE	Butyrylcholinesterase
CTX	Cyclophosphamide
IFN-γ	Interferon-γ
hCES1A	Human carboxylesterase 1A1
MMP-1	Matrix metalloproteinase-1
PTP1B	The protein tyrosine phosphatase 1B
iNOS	Nitric oxide synthase
TNF-α	Tumor necrosis factor-α
IL-1β	Interleukin-1β
COX-1	Cyclooxygenase-1
COX-2	Cyclooxygenase-2
NF-κb	Nuclear factor-κB

Appendix A

Table A1.

Genus Styrax and their traditional use.

Genus Styrax	Traditional Use
S. perkinsiae	Estrogen synthesis promotion
S. obassia	Anticancer activities
S. japonica	Cough, bronchitis, sore throat, inflammation, paralysis, laryngitis, and expectoration treatment
S. pohlii	/
S. camporum	Antimicrobial, anticancer, antifungal, hypolipidemic, and immunossupressive activities
S. macranthus	/
S. officinalis	Antiseptic and anti-respiratory disease
S. argentifolius	/
S. ferrugineus	/
S. agrestis	/
S. tonkinensis	Expectorant, antiseptic, and analgesic activities
S. suberifolius	Rheumatic disease cure
S. ramirezii	/

Appendix B

Table A2.

Chemical constituents of plants from the genus Styrax.

No.	Compound Class and Name	Source	Ref.
Lignans
1	5-(2-Propen-1-one)-7-me-thoxy-2-(3,4-methylenedioxyphenyl) benzofuran	S. perkinsiae	[18]
2	1″-Hydryoxyegonol gentiobioside	S. perkinsiae	[18]
		S. obassia	[2]
		S. ferrugineus	[19]
3	Obassioside B	S. perkinsiae	[18]
		S. obassia	[2]
		S. obassia S. obassia	[2,60]
4	Egonol	S. perkinsiae	[18]
		S. japonica	[21,25]
		S. obassia	[28,31]
		S. agrestis	[10]
		S. ferrugineus	[19]
		S. pohlii	[69]
		S. camporum	[11]
		S. macranthus	[32]
		S. officinalis L.	[35]
		S. argentifolius	[43]
5	Egonol glucoside	S. perkinsiae	[18]
		S. japonica	[20]
		S. suberifolius	[40]
		S. macranthus	[32]
		S. obassia	[28]
			[60]
6	Egonol gentiobioside	S. perkinsiae	[17,18]
		S. macranthus	[32]
		S. obassia	[2]
		S. pohlii	[69]
7	Egonol gentiotrioside	S. perkinsiae	[17,18]
		S. macranthus	[32]
8	MasutakesideI (sutakeside I)	S. perkinsiae	[18]
		S. japonica	[22]
		S. obassia	[27]
9	trans-5-(3-Hydroxypropyl)-7-methoxy-2-[3-methoxy-4-hydroxyphenyl)-benzofuran-5-yl]benzufuran	S. perkinsiae	[17]
10	(E)-5-(2-Formylvinyl)-7-metho-xy-2-(3,4-methylenedioxyphenyl)benzofuran	S. perkinsiae	[17]
11	5-(3-Butanoyloxypropyl)-7-methoxy-2-(3,4-methylene dioxyphenyl)benzofuran	S. perkinsiae	[17]
12	5-(3-Hydroxypropyl)-7-hydroxy-2-(3,4-methylene-dioxyphenyl) benzofuran	S. perkinsiae	[17]
		S. macranthus	[32]
12	5-(3-Hydroxypropyl)-7-hydroxy-2-(3,4-methylene-dioxyphenyl) benzofuran	S. perkinsiae	[17]
		S. macranthus	[32]
13	Egonol acetate	S. perkinsiae	[17]
		S. japonica	[25]
		S. obassia	[28,29,31]
		S. agrestis	[10]
		S. officinalis L.	[37]
14	Demethoxy egonol acetate	S. perkinsiae	[17]
		S. japonica	[25]
15	Styraxlignolide B	S. perkinsiae	[18]
		S. japonica	[7]
16	Styraxjaponoside C	S. perkinsiae	[18]
		S. japonica	[7]
17	Nor-lignans5-(3″-hydroxypropyl)-7-methoxy-2-(3′,4′-methylenedioxyphenyl) benzofuran	S. ferrugineus	[19]
		S. camporum	[11]
		S. pohlii	[69]
18	5-[3″-(β-D-Glucopyranosyloxy)propyl]-7-methoxy-2-(3′,4′-methylenedioxyphenyl) benzofuran	S. ferrugineus	[19]
		S. pohlii	[69]
19	Dihydrodehydrodiconiferyl alcohol	S. ferrugineus	[19]
20	Styraxjaponoside C	S. japonica	[21]
21	Arctiin	S. japonica	[21]
22	Matairesinoside	S. japonica	[20,21]
23	Pinoresinol-4-O-β-D-glucopyranoside	S. japonica	[21]
24	2R-(4′-hydroxy-3′-methoxyphenyl)-6R-(3″,4″-methylenedioxyphenyl)-8-oxo-3,7-dioxabicyclo [3.3.0]octane 4′-hydroxyl	S. japonica	[7]
25	Styraxjaponoside A	S. japonica	[20]
26	Styraxjaponoside B	S. japonica	[20]
27	Dihydrodehydrodiconiferyl alcohol 9-O-glucoside	S. japonica	[20]
28	Styraxlignolide A	S. japonica	[22]
29	Styraxlignolide B	S. japonica	[7]
30	Styraxlignolide C	S. japonica	[7]
31	Styraxlignolide D	S. japonica	[7]
32	Styrax lignolides F	S. japonica	[7,24]
33	(–)-Pinoresinol glucoside	S. japonica	[7]
34	Styrlignan A	S. japonica	[23]
35	1R,2R,5S,6R-2-(4′-Hydroxy-3′-methoxyphenyl)-6-(3″,4″-dimethoxyphenyl)-3,7-dioxabicyclo-[3.3.0]octane4′-O-β-D-glucopyranoside	S. japonica	[24]
36	Obassioside A	S. japonica	[26]
37	Methyl 3-[7-methoxy-2-(3′,4′-methylene-dioxyphenyl)-5-benzofuranyl]-propionate	S. obassia	[31]
38	Methyl3-[2-(3′,4′-methylen-edioxyphenyl)-5-benzofuranyl]-propionate	S. obassia	[31]
39	5-(3″-Propanoyloxypropyl)-7-methoxy-2-(3′,4′-methylenedioxyphenyl)-benzofuran	S. obassia	[28]
		S. agrestis	[10]
40	Egonol-2-methylbutanoate	S. obassia	[31]
41	7-Demethoxylegonol-2-methylbutanoate	S. obassia	[5]
42	Egonol propanoate	S. obassia	[29]
		S. agrestis	[10]
43	3-[7-Methoxy-2-(3,4-methylenedioxyphenyl)benzofuran-5-yl]propyl-3-[7-methoxy-2-(3,4-methylenedioxyphenyl)-benzofuran-5-yl]propanoate	S. macranthus	[32]
44	Demethoxy egonol gentiobioside	S. macranthus	[32]
45	7-Methoxy-2-(3,4-methylenedioxyphenyl) benzofuran-5-carbaldehyde	S. macranthus	[32]
46	Demethoxy egonol	S. macranthus	[32]
		S. obassia	[28]
		S. japonica	[23]
47	5-3″-(2-Methylbutanoyloxy)propyl]-7-methoxy-2-(3′,4′-dimethoxyphenyl) benzofuran	S. officinalis L.	[23]
48	5-(3″Benzoyloxypropyl)-7-methoxy-2-(3′,4′-methylenedioxyphenyl)-benzofuran	S. officinalis L.	[36]
49	4-[3″-(1c-methylbutanoyloxy)propyl]-2-methoxy-(3′,4′-methylenedioxyphenyl)-1a,5b-dihydrobenzo-[3,4]-cyclobutaoxirene	S. officinalis L.	[65]
50	Egonol-9(Z),12(Z) linoleate	S. agrestis	[10]
51	7-Demethoxyegonol-9(Z),12(Z) linoleate	S. agrestis	[10]
52	7-Demethoxyegonol oleate	S. agrestis	[10]
53	Egonol oleate	S. agrestis	[10]
54	7-Demethoxylegonol acetate	S. agrestis	[10]
55	Egonol-2-methylpropanoat	S. agrestis	[10]
56	(±)Syringaresinol	S. camporum	[38]
57	Homoegonol gentiobioside	S. pohlii	[39]
58	Lariciresinol 4-O-β-D-glucoside	S. perkinsiae	[39]
59	(−)-Secoisolariciresinol 4-O-β-D-Glucopyranoside	S. perkinsiae	[39]
60	Lariciresinol4′-O-β-D-glucoside	S. perkinsiae	[39]
61	Lanicepside A	S. perkinsiae	[39]
62	Solariciresinol4-O-β-D-glucopyranoside	S. perkinsiae	[39]
63	(+)-Lariciresinol9-O-β-D-glucopyranoside	S. perkinsiae	[39]
64	2R,3S-Dihydrodehydrodiconiferyl alcohol 4′-O-β-D-glucopyranoside	S. perkinsiae	[39]
65	Pinoresinol	S. perkinsiae	[39]
66	Homoegonol glucoside	S. suberifolius	[40]
67	2-(4-Hydroxy3-methoxyphenyl)-5-(3-hydroxypropyl)-7-methoxybenzofuran	S. suberifolius	[40]
68	2-(3-Hydroxy-4-methoxyphenyl)-7-methoxy-5benzofuranpropanol	S. suberifolius	[40]
69	(+)-Cedrusin	S. suberifolius	[40]
70	(−)-(7R,8S)-Dihydrodehydrodiconiferyl alcohol	S. suberifolius	[40]
71	(−)-(7R,8S)-Dihydrodehydrodiconiferyl alcohol 4-O-β-Dglucopyranoside	S. suberifolius	[40]
72	(−)-(7S,8R)-Dihydrodehydrodiconiferylalcohol 4-O-β-D-glucopyranoside	S. suberifolius	[40]
73	(+)-(7S,8R)-Dihydrodehydrodiconiferyl alcohol	S. suberifolius	[40]
74	(+)-(7S,8R)-Erythro-4,7,9,9′-tetrahydroxy-3,3′-dimethoxy-8-O-4′-neolignan	S. suberifolius	[40]
75	(−)-Symplocosneolignan A	S. suberifolius	[40]
76	(−)-7-O-Ethylguaiacylglycerol (15)	S. suberifolius	[40]
77	2[4-(3-Hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol	S. suberifolius	[40]
78	Dihydroconiferyl alcohol	S. suberifolius	[40]
79	3,3-Bis(3,4-dihydro-6-methoxy-2H-1-benzopyran	S. tonkinensis	[41]
80	Rac-(8α,8′β)-4,4′-dihydroxy3,3′-dimethoxylignan-9,9′-diyldiacetate	S. tonkinensis	[41]
81	(–)-Secoisolariciresino	S. tonkinensis	[41]
82	4,4′-Dihydroxy-3,3′dimethoxy-9-ethoxy-9,9′-epoxylignan	S. tonkinensis	[41]
83	(2S,3R,4R)-4-[1-Ethoxy-1-(4-hydroxy-3-methoxy)phenyl]methyl-2(4-hydroxy-3-methoxy)phenyl-3-hydroxymethyl-tetrahydrofuran	S. tonkinensis	[41]
84	(–)-Neo-olivil-(9-O-9″)-seco-isolariciresinol	S. tonkinensis	[41]
85	Isolariciresinol	S. tonkinensis	[41]
86	Stytonkinol A	S. tonkinensis	[42]
87	Stytonkinol B	S. tonkinensis	[42]
88	Styraxin	S. argentifolius	[43]
89	Vladinol D	S. argentifolius	[43]
90	5-Carboxy7-methoxy-2-(3′,4′-methylenedioxyphenyl)benzofuran	S. argentifolius	[43]
91	5-((E)-2-Carboxyvinyl)-7-methoxy-2-(3′,4′-methylenedioxyphenyl)benzofuran	S. argentifolius	[43]
92	(–)-Machicendiol	S. argentifolius	[43]
93	Machicendona	S. argentifolius	[43]
Terpenoids
94	Taraxerol	S. japonica	[7,45]
95	Oleanolic aldehyde acetate	S. japonica	[22,54]
96	Erythrodiol-3-acetate	S. japonica	[22,54]
97	Euphorginol	S. japonica	[22,54]
98	3β-Acetoxyolean-12-en-28-acid	S. japonica	[45]
99	3β-Acetoxy-17β-hydroxy-28-norolean-12-ene	S. japonica	[45]
100	Anhydrosophoradiol-3-acetate	S. japonica	[22,54]
101	Styraxosides B	S. japonica	[44]
102	Camellenodiol	S. japonica	[44]
103	Jegosaponins A	S. japonica	[46]
104	Jegosaponins B	S. japonica	[46]
105	Jegosaponins C	S. japonica	[46]
106	Jegosaponins D	S. japonica	[46]
107	3β-Acetoxy-28-hydroxyolean-12-ene	S. japonica	[47]
108	3β-Acetoxyolean-12-en-28-acid	S. japonica	[47]
109	3β-Acetoxyolean-12-en-28-aldehyde	S. japonica	[47]
110	3β-Acetoxy-17β-hydroxy-28-norolean-12-ene	S. japonica	[47]
111	6β-Hydroxy-3-oxo-11α,12α-epoxyolean- 28,13β-olide	S. tonkinensis	[4]
112	3β,6β-Dihydroxy-11α,12α-epoxyolean-28,13β-olide	S. tonkinensis	[4]
113	3β,6β-Dihydroxy-11-oxo-olean-12-en-28-oic acid	S. tonkinensis	[4]
114	3β-Hydroxy-12-oxo-13HR-olean-28,19β-olide	S. tonkinensis	[4]
115	19β-Hydroxy-3-oxo-olean-12-en-28-oic acid	S. tonkinensis	[4]
116	6β-Hydroxy-3-oxo-olean-12-en-28-oic acid	S. tonkinensis	[4]
117	Sumaresinolic acid	S. tonkinensis	[4,51]
118	Siaresinolic acid	S. tonkinensis	[4,51]
119	Oleanolic acid	S. tonkinensis	[4,51]
120	21-Benzoylbarringtogenol C	S. officinalis L.	[4]
121	Styrax-saponin A	S. officinalis L.	[49]
122	Styrax-saponin B	S. officinalis L.	[49]
123	Styrax-saponin C	S. officinalis L.	[49]
124	Deacylsaponin	S. officinalis L.	[49]
125	Oleanonic acid	S. tonkinensis	[42,51]
126	Betulonic acid	S. tonkinensis	[42,51]
127	Corosolic acid	S. tonkinensis	[42,51]
128	Maslinic acid	S. tonkinensis	[42,51]
129	Epibetulinic acid	S. tonkinensis	[42]
130	Betulinic acid	S. tonkinensis	[42,51]
131	2α,3α,24-trihydroxy-urs-12-en-28-oic acid	S. argentifolius	[43]
Aromatic Compounds
132	trans-(Tetrahydro-2-(4-hydroxy-3-methoxyphenyl)-5-oxofuran-3-yl)methylbenzoate	S. tonkinensis	[52]
133	3-(4-Hydroxy-3-methoxyphenyl)-2-oxopropylbenzoate	S. tonkinensis	[52]
134	4-(E)-3-Ethoxyprop-1-enyl)-2-methoxyphenol	S. tonkinensis	[52]
135	Benzoic acid	S. tonkinensis	[52]
136	Vanillin	S. tonkinensis	[52]
137	Dehydrodivanillin	S. tonkinensis	[52]
138	Vanillic acid	S. tonkinensis	[52]
139	Coniferyl aldehyde	S. tonkinensis	[52]
140	Methylsyringin	S. japonica	[21]
141	Syringin	S. japonica	[7,21]
142	Isotachioside	S. perkinsiae	[39]
143	(2R,3R)-3,7,4′-Trihydroxy-5,3′-dimethoxyflavan 7-O-β-D-glucopyranoside	S. suberifolius	[40]
144	Methyl orsellinate	S. suberifolius	[40]
145	Ethyl orsellinate	S. suberifolius	[40]
146	Methyl β-orcinolcarboxylate	S. suberifolius	[40]
Steroids
147	Stigmasterol	S. macranthus	[32]
148	Styraxosides A	S. japonica	[32]
149	Daucosterol	S. macranthus	[32]
150	β-Sitosterol	S. perkinsiae	[17]
151	(20R)-24Ethylcholest-5,22-dien-7-one	S. argentifolius	[43]
Others
152	Kaempferol-3-O-(2″,4″-di-O-(E)-p-coumaroyl-β-D-glucopyranoside)	S. pohlii	[56]
		S. camporum	[56]
153	Kaempferol-3-O-(2″,6″-di-O-(E)-p-coumaroyl)-β-D-glucopyranoside	S. pohlii	[56]
		S. camporum	[56]
154	Quercetin	S. pohlii	[56]
		S. camporum	[57]
155	Kaempferol	S. pohlii	[56]
		S. camporum	[57]
156 157	Koninginin T Koninginin U	S. pohlii	[57]
		S. pohlii	[57]
158	Jegosaponins A	S. japonica	[58]
159	Jegosaponins B	S. japonica	[58]

Appendix C

Table A3.

Structural information of the active molecule.

No.	Structure	Phytochemistry	Pharmacological Activity
3		S. perkinsiae S. obassia	MCF-7 (IC50 = 53.5 μg/mL) Hela (IC50 = 23.3 μg/mL) HL-60 (IC50 = 16.8 μg/mL)
4		S. perkinsiae S. japonica S. obassia S. agrestis S. ferrugineus S. pohlii S. camporum S. macranthus S. officinalis S. argentifolius	Hep-2 (IC50 = 3.6 μg/mL) C6 (IC50 = 3.2 μg/mL) KB (IC50 = 22.11 μg/mL) HepG2 (IC50 = 18.15 μg/mL) Lu (IC50 = 21.50 μg/mL) Hemolytic activity (IC50 = 33 μg/mL) C. sphaerospermum (MIC = 5 mg/mL) C. albicans (MIC = 10 μg/mL) S. aureus (MIC = 10 μg/mL) ATCC 27853 and ATCC 6305 (MIC = 400 mg/mL) COX-1 (inhibition rate = 35.7% at 30 μM) Reduce doxorubicin- and methanesulfonate-induced DNA and chromosomal damage
5		S. perkinsiae S. japonica S. suberifolius S. macranthus S. obassia	MCF-7 (IC50 = 27.9 μg/mL) Hela (IC50 = 23.3 μg/mL) S. aureus (MIC = 20 μg/mL) C. albicans (MIC = 15 μg/mL) HL-60 (IC50 = 47.8 μg/mL)
6		S. perkinsiae S. macranthus S. obassia S. pohlii	Estrogen biosynthesis
7		S. perkinsiae S. macranthus	Estrogen biosynthesis
10		S. perkinsiae	Hemolytic activity (IC50 = 166 μg/mL)
11		S. perkinsiae	MCF-7 (IC50 = 5.5 μg/mL) MDA-MB-231 (IC50 = 3.81 μg/mL)
14		S. perkinsiae S. japonica	MCF-7 (IC50 = 15.0 μg/mL) MDA-MB-231 (IC50 = 13.71 μg/mL)
17		S. ferrugineus S. camporum S. pohlii	HeLa (IC50 = 5.3 μg/mL) C6 (IC50 = 4.9 μg/mL) C. sphaerospermum (MIC = 10 μg/mL) C. albicans (MIC = 12 μg/mL) S. aureus (MIC = 10 μg/mL) Th2 cytokines, iNOS, MMP-9 Reduce doxorubicin- and methanesulfonate-induced DNA and chromosomal damage
18		S. ferrugineus S. pohlii	S. aureus (MIC = 20 μg/mL) C. albicans (MIC = 20 μg/mL)
19		S. ferrugineus	S. aureus (MIC = 20 μg/mL) C. albicans (MIC = 15 μg/mL)
26		S. japonica	MMP-1 (inhibition rate = 62.1% at 10 μM)
28		S. japonica	Hemolytic activity (IC50 = 123 μg/mL)
30		S. japonica	DPPH (IC50 = 380 μM)
31		S. japonica	DPPH (IC50 = 278 μM)
32		S. japonica	DPPH (IC50 = 194 μM)
33		S. japonica	DPPH (IC50 = 260 μM)
50		S. agrestis	EeAChE (IC50 = 1.4 μg/mL) hAChE (IC50 = 1.7 μg/mL)
51		S. agrestis	EeAChE (IC50 = 2.0 μg/mL) hAChE (IC50 = 2.7 μg/mL)
52		S. agrestis	EeAChE (IC50 = 1.4 μg/mL) hAChE (IC50 = 1.8 μg/mL)
53		S. agrestis	EeAChE (IC50 = 2.2 μg/mL) hAChE (IC50 = 3.1 μg/mL)
86		S. tonkinensis	Hela (IC50 = 26.75 μM) MCF-7 (IC50 = 45.16 μM)
87		S. tonkinensis	MCF-7 (IC50 = 57.1 μM) Hemolytic activity (IC50 = 65 μg/mL)
92		S. argentifolius	KB (IC50 = 96.01 μg/mL) HepG2 (IC50 = 86.60 μg/mL) Lu (IC50 = 106.86 μg/mL)
95		S. japonica	Human dermal fibroblasts (IC50 = 20 μM)
96		S. japonica	MMP-1 (inhibition rate = 73.1% at 0.01 μM)
100		S. japonica	Human dermal fibroblasts (IC50 = 1.12 μM)
101		S. tonkinensis	Hemolytic activity (IC50 = 65 μg/mL)
108		S. japonica	PTP1B (IC50 = 7.8 μg/mL)
109		S. japonica	PTP1B (IC50 = 9.3 μg/mL)
112		S. tonkinensis	HL-60 (IC50 = 27.5 μg/mL)
116		S. tonkinensis	HL-60 (IC50 = 14.2 μg/mL)
118		S. tonkinensis	HL-60 (IC50 = 29.0 μg/mL)
119		S. tonkinensis	HL-60 (IC50 = 8.9 μg/mL)
125		S. tonkinensis	hCES1A (IC50 = 0.49 mg/mL)
126		S. tonkinensis	hCES1A (IC50 = 1.48 μg/mL)
129		S. tonkinensis	hCES1A (IC50 = 0.041 μg/mL)
144		S. suberifolius	A. solani (inhibition rate = 58.41% at 100 μg/mL) F. oxysporum (inhibition rate = 67.39% at 100 μg/mL)
145		S. suberifolius	A. solani (inhibition rate = 59.31% at 100 μg/mL) F. oxysporum (inhibition rate = 45.65% at 100 μg/mL)
146		S. suberifolius	F. oxysporum (inhibition rate = 61.41% at 100 μg/mL) P. cytospore (inhibition rate = 86.72% at 100 μg/mL)
149		S. macranthus	iNOS, COX-2, TNF-a, and IL-1b
152		S. pohlii S. camporum	Separate coupled Schistosoma mansoni adult worms
155		S. pohlii S. camporum	Separate coupled Schistosoma mansoni adult worms Kill adult schistosomes
156		S. pohlii	AChE
158		S. japonica	Hemolytic activity (IC50 = 2.1 μg/mL)
159		S. japonica	Hemolytic activity (IC50 = 20.2 μg/mL)

Author Contributions

Conceptualization, D.-D.X. and X.-Y.H.; methodology, Y.Z.; formal analysis, N.Z.; investigation, D.-D.X. and X.-Y.H.; resources, Y.Z.; data curation, D.-D.X.; writing—original draft preparation, D.-D.X. and X.-Y.H.; writing—review and editing, D.-D.X., X.-Y.H. and Y.Z.; visualization, N.Z.; supervision, N.Z.; project administration, Y.Z.; funding acquisition, Y.Z. All authors have read and agreed to the published version of the manuscript.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

Funding Statement

This work was supported by the Chenguang Program of the Shanghai Education Development Foundation and the Shanghai Municipal Education Commission (22CGA51 to Y.Z.). And the start-up funding from Shanghai University of Traditional Chinese Medicine to Y.Z.