Towards Early Diagnosis of Mixed Connective Tissue Disease: Updated Perspectives

Abstract

Mixed Connective Tissue Disease (MCTD) is an autoimmune disease first described by Sharp et al in 1972, characterized by the presence of anti-Ribonucleoprotein antibodies directed against the U1 complex (anti-U1RNP). The condition shares clinical characteristics with Systemic Lupus Erythematosus, Rheumatoid Arthritis, and Systemic Sclerosis. Diagnosis is quite difficult due to its rarity, the lack of validated classification criteria, and its heterogeneous clinical presentation. While in the early stages its nuanced clinical features might lead to it being incorrectly classified as other Connective Tissue Diseases (CTDs) or even not recognized, in cases of longstanding disease its classification as a CTD is clear but challenging to discriminate from overlap syndromes. MCTD should be considered a distinct entity due to the presence of a specific genetic substrate and the presence of the high titer of a specific autoantibody, anti-U1RNP, present in all the commercial kits for Extractable Nuclear Antigens, and almost always associated with Antinuclear Antibody positivity with a coarse speckled pattern. Except for anti-U1RNP, no specific biomarkers are available to guide clinicians to a correct classification of MCTD, which is arrived at by the association of clinical, serological and instrumental evaluation. In the first stages, the disease is mainly characterized by Raynaud’s phenomenon, inflammatory arthritis, puffy fingers, myalgia and/or myositis, and rarely, trigeminal neuropathy. Longstanding disease is generally associated with the development of Pulmonary Hypertension and Interstitial Lung Disease, which are the two main causes of mortality in MCTD. The aim of this review is to summarize current knowledge on the early recognition of MCTD.

Introduction

The term “Mixed Connective Tissue Disease” (MCTD) refers to a rare autoimmune disease, first described by Sharp et al in 1972, that shares clinical features with Polymyositis (PM), Systemic Sclerosis (SSc), Systemic Lupus Erythematosus (SLE), and Rheumatoid Arthritis (RA).¹ Despite its heterogeneous clinical presentation, it is generally accepted that MCTD should be considered a distinct clinical entity rather than an overlap condition, being associated with a specific autoantibody: anti-U1-Ribonucleoprotein (anti-U1RNP).¹

The epidemiology of MCTD is affected by several potential biases, due to its rarity, heterogeneous presentation, and the presence of multiple subsets of proposed classification criteria to define the condition.^2–6 Referring to the two studies that included patients enrolled according to the four subsets of classification criteria available at the time of the study,^7,8 the incidence of MCTD is reported at 2.1–19 per million per year, with a prevalence of about 3 per 100,000 persons, and a female-to-male ratio of 3–5:1. Despite its multi-organ involvement, mortality was described as similar to the general population.⁷

The pathophysiology of this condition is also largely unknown, but it clearly seems to be multifactorial. A genetic association with the development of MCTD was found in patients with HLA-DR4, but not with HLA-DR3 and -DR5 (which are strongly associated with SLE and SSc respectively).⁹ This data could further support the definition of MCTD as a distinct entity. Environmental and occupational exposure in predisposed patients, as well as infections (mainly retroviruses such as Human Immunodeficiency Virus Type-1) seem to play a critical role in the development of the condition.^10,11 The hallmark of the disease is represented by anti-U1RNP (or anti-nRNP),¹² an autoantibody directed against the 70kDa subunit of the U1 RNP complex, an intracellular protein involved in mRNA maturation.¹³ It is possible that during the process of apoptosis, a modified U1 70kDA protein is exposed on the surface of the apoptotic blebs, which is then recognized by antigen-presenting cells, presented to T cells, leading ultimately to B cell stimulation.¹⁴ Anti-U1-RNP can stimulate both mononuclear cells and autoreactive CD4+ cells, with an increased production of Interleukin (IL) 1 and IL6 (leading to autoimmune inflammation), but tissue injury is also sustained due to the direct action of the autoantibody on endothelial cells. It is able to induce the production of intercellular and endothelial leukocyte adhesion molecule-1 (ICAM-1 and ELAM-1, respectively) which also act as anti-endothelial cells.¹⁴ This mechanism could explain the vascular involvement seen in MCTD, in particular Pulmonary Artery Hypertension (PAH). The presence of double stranded RNA in the U1 complex is responsible for the activation of innate immunity through Toll-like Receptor 3, activating also the innate immunity.¹⁴ Anti-U1RNP also seems to be able to activate immature dendritic cells via TLR7, inducing the production of Type I Interferon (IFN-1), in turn able to promote the activation of autoreactive T cells, with a down-regulation of regulatory T cells and the production of effector T cells, ultimately responsible of the tissue injury. IFN-1 also activates autoreactive T cells, leading to the production of autoantibodies.¹⁵ Anti-U1RNP seems also have a prognostic value: high titre of anti-U1RNP seems to be associated with the presence of PAH and a less common neurological involvement, whereas its disappearance is associated with a persistent disease remission.^15–17

Clinical Presentation and Classification Criteria

The initial clinical picture of MCTD patients is generally characterized by non-specific symptoms such as low-grade fever, arthralgia and fatigue.

Generally, the first specific involvement includes the skin. MCTD is potentially associated with a skin involvement common in SLE and above all SSc, therefore it is almost impossible to distinguish between these two conditions through a simple clinical evaluation. The first symptom is generally Raynaud’s Phenomenon (RP). During follow-up, any of the typical clinical features of SSc can occur, from puffy hands to sclerodactyly, acrosclerosis, calcinosis, ulcers and digital necrosis.¹⁸ RP is present in almost all MCTD patients from the first assessment onwards, whereas fibrotic changes of the skin became more common during follow-up, reaching a prevalence of about 90%.¹⁹ Oral ulcers, discoid lesions, panniculitis and the classical SLE malar rash are also common in MCTD.¹⁹

Inflammatory arthritis is also a common clinical feature in MCTD, generally present from the first stage of the disease onward. Joint involvement resembles classic RA, and positivity for Rheumatoid Factor (RF) and Anti Citrullinated Protein Antibody (ACPA) are noted in 70% and 50% of patients respectively.²⁰ This inflammatory arthritis is potentially erosive, potentially leading to the typical deformities associated with RA.²¹

Inflammatory myopathy is also very common and is necessary, under all of the proposed criteria, for classification as MCTD. Also in this case, MCTD might be clinically indistinguishable from an idiopathic inflammatory myopathy, with proximal weakness, typical alteration at electromyography and increased serum level of muscle enzymes such as Creatine Phosphokinase (CPK), Lactic Dehydrogenase (LDH), transaminases.²² Muscle samples from MCTD patients show muscle cellular injury similar to that shown in PM patients, together with the vascular changes reported in Dermatomyositis patients.²³ It should be noted that most patients only report diffuse myalgia, which could be the expression of mild, silent, or localized myositis.²⁴

From the gastrointestinal point of view, MCTD shows involvement that is common in SSc, SLE and PM. Gastroesophageal Reflux with heartburn and dysphagia is present in about 50% of patients, and is the most common feature.²⁵ Almost any part of the gastrointestinal tract could be involved during MCTD, although generally it is very uncommon. Peristalsis disturbance, hepatitis, pancreatitis, malabsorption and small bowel perforation secondary to vasculitis are also possible.²⁶

The cardiovascular system is also commonly involved. Pericarditis is present, though often subclinically, in about 40% of patients, while heart rhythm disturbances affect about 20%.²⁷ A large proportion of patients show intimal proliferation and medial hypertrophy of medium- and small-sized vessels.²⁸ This mechanism, characteristic of the peripheral vasculopathy responsible for ulcers in the fingers, is potentially also present in the pulmonary vasculature, leading to PAH. PAH is present in about 20% of MCTD patients and represents one of the main causes of mortality.²⁹ Other potential mechanisms associated with PH are chronic embolism and Interstitial Lung Disease (ILD).³⁰

Lung involvement is also very common. The most important condition in terms of prevalence and prognostic impact is ILD, affecting about 60% of MCTD patients.³¹ Patients display features quite similar to those reported in SSc: High-Resolution Computed Tomography (HRCT) shows the prevalence of a fibrotic Nonspecific Interstitial Pneumonia (NSIP) pattern (in nearly 90% of patients), whereas about 10% of patients have a definite Usual Interstitial Pneumonia (UIP) pattern.³²

Figure 1 shows a typical fibrotic NSIP pattern in a patient with longstanding MCTD.

Figure 1.

Fibrotic Nonspecific Interstitial Pneumonia in a patient with a longstanding Mixed Connective Tissue Disease. The pattern is characterized by diffuse Ground Glass Opacities (yellow arrow), subpleural sparing (blue arrow), and presence of traction bronchiectasis (red arrow).

Pleurisy is also reported in about 50% of patients, and it can be a rarely presenting manifestation of MCTD, but is generally mild and does not require specific treatment.³³ Rare and severe manifestations are, as reported for SLE: pulmonary vasculitis, pulmonary hemorrhage and thromboembolism associated with Anti-Phospholipid Syndrome (APS).³³

From the neurological point of view, as reported for SSc, the most common presentation is trigeminal neuropathy. It is present in about a quarter of patients, is a possible clinical presentation of MCTD, and was included in the last version of proposed classification criteria.^6,34,35 Other manifestations are generally mild, characterized by headaches, cognitive impairment, depression, and hearing loss. Similarly to SLE, psychosis, seizure and encephalitis are possible.³⁴

MCTD patients may also experience kidney damage. It is present in about 25% of patients, mainly characterized by membranous nephropathy, but is generally mild.³⁶ In fact, anti-RNP could have a protective role against kidney involvement in both MCTD and SLE, irrespective from the titre.³⁷ Other kidney manifestations, typical of SLE and SSc (including scleroderma renal crisis) are also reported.³⁶

Finally, MCTD patients may also experience hematologic manifestations. The most common are hemolytic anemia, autoimmune thrombocytopenia and leukopenia, as also reported for SLE.¹⁹ Anticardiolipin antibodies can be also present and they could be associated with APS, whereas some case reports describe thrombotic thrombocytopenic purpura.¹⁹

The combination of these clinical findings allows for a classification as MCTD. As things currently stand, the American College of Rheumatology and the European League Against Rheumatism, the most important societies in rheumatology, have not yet provided a specific classification of MCTD. Of the four historical classification criteria,^2–5 the maximum sensitivity was shown by the Kasukawa criteria (77.5%) whereas the best specificity was found for the sets proposed by Alarcón-Segovia-Villareal and Kahn (both 99.4%).³⁸ In addition, a relatively recent new set of diagnostic criteria was proposed in 2019.⁶

All of these criteria are reported in Table 1.

Table 1.

Proposed Classification Criteria for Mixed Connective Tissue Disease

Sharp GC2	Khan MF et al5	Alarcon-Segovia and Villareal3	Kasukawa R et al4	Tanaka Y et al6
Major criteria ● Severe myositis ● Pulmonary involvement – DLCO <70% of the predicted – PAH – Proliferative vascular lesions at the biopsy ● RP or esophageal hypomotility ● Edema of the hands or sclerodactyly ● Anti-ENA antibodies ≥1:10,000 and anti-nRNP positive and anti-Sm Negative	Serological Anti-n-RNP antibodies titer >1:1.600	Serological Anti-n-RNP antibodies titer >1:1.600	Common symptoms RP Edema of the hands or fingers only Anti n-RNP antibodies positive	I. Common manifestations  1. RP  2. Puffy fingers and/or swollen hands II. Immunological manifestation  Positivity for anti-U1RNP III. Characteristic organ involvement  1. PAH  2. Aseptic meningitis  3. Trigeminal neuropathy IV. Overlapping manifestations A. SLE-like manifestations  1. Polyarthritis  2. Lymphadenopathy  3. Malar rash  4. Pericarditis or pleuritis  5.Leukopenia (4000/μL or less) or thrombocytopenia (100,000/μL or less) B. SSc-like manifestations  1. Sclerodactyly  2. Interstitial lung disease  3. Esophageal dysmotility or dilatation C.PM/DM-like manifestations  1. Muscle weakness  2. Elevated levels of myogenic enzymes  3. Myogenic abnormalities on EMG
Minor criteria ● Alopecia ● Leukopenia ● Anemia ● Pleurisy ● Pericarditis ● Arthritis ● Trigeminal neuropathy ● Malar rash ● Thrombocytopenia ● Moderate myositis ● Edema of the hands in the past	Clinical Swollen hands Synovitis Myositis RP	Clinical ● Edema of the hands ● Synovitis ● Myositis ● RP ● Acrosclerosis	Mixed symptoms ● SLE-like manifestations: – Polyarthritis – Lymphadenopathy – Erythema of the face – Pericarditis or pleuritis – Leukopenia or thrombocytopenia ● SSc-like events: – Sclerodactyly – Pulmonary fibrosis, changes restrictive or reduction of DLCO – Hypomotility or esophageal dilatation ● PM manifestations – Muscle fatigue – Elevated CPK levels – Myogenic pattern on EMG
For diagnosis: – 4 major criteria + anti-RNP titer >1:4000 (exclusion criterion: positivity for anti-Sm); – 2 among the first three major criteria + 2 minor criteria + anti-nRNP ≥1:1000	For diagnosis: Serological criterion + RP + ≥ 2 remaining clinical criteria	For diagnosis: Serological criteria + at least 3 clinical criteria	For diagnosis: At least 1 of the common symptoms + positivity for anti-nRNP + at least 1 mixed symptom of at least 2 or 3 categories of manifestation	For diagnosis: At least one common manifestation, immunological manifestation, and at least one characteristic organ involvement; or at least one common manifestation, immunological manifestation, and at least one feature each in 2 or more from items A, B, and C in overlapping manifestations.
Sensitivity 57.7%, specificity 90%34	Sensitivity 52.3%, specificity 99.4%34	Sensitivity 69.4%, specificity 99.4%34	Sensitivity 77.5%, specificity 92.2%34	Sensitivity 90.6%, specificity 98.4%6

Abbreviations: anti-Sm, Anti Smith antigen; anti-U1RNP, anti-U1 ribonucleoprotein antibody; CPK, Creatine Phosphokinase; DLCO, Diffusion Lung Capacity for Carbon Monoxide; DM, Dermatomyositis; EMG, Electromyography; ENA, Extractable Nuclear Antigen; PAH, Pulmonary Arterial Hypertension; PM, Polymyositis; RP, Raynaud’s Phenomenon; SLE, Systemic lupus erythematosus; SSc, Systemic Sclerosis.

Early Diagnosis of MCTD

MCTD is a rare condition, and diagnosis is further complicated by the presence of overlapping features with other conditions. Distinguishing MCTD from other systemic rheumatic diseases, including overlap syndromes, is a clinical challenge due to the heterogeneity of disease presentations; misdiagnosis at presentation of the disease is thus common in up to 61% of a single center cohort. Undifferentiated CTD, non-MCTD overlap syndromes and SLE were the most frequent diagnoses mistaken for MCTD that were reported.³⁹ Some cases of MCTD have been shown to evolve over time into other systemic rheumatic diseases,⁴⁰ while other studies have reported that true phenotypic conversion is rare.³⁹

In the first stage of the disease, the challenge for physicians is to consider the presence of MCTD in patients with nuanced clinical features. Classical clinical presentation at onset includes RP, inflammatory arthritis, puffy fingers and, rarely, trigeminal neuropathy. These parameters, when present simultaneously, even if not sufficient to reach specific classification criteria, clearly suggest an autoimmune origin. However, when only inflammatory arthritis is present, inaccurate classification is possible, whereas in cases of an isolated onset of RP, clinicians should also consider the fact that this sign is also prevalent in the general population.

In advanced stages, the clinical picture unquestionably indicates the presence of a Connective Tissue Disease (CTD), however the difficultly lies in determining which cases are actually MCTD and not another specific condition such as SLE, SSc, or commonly, an overlap condition.

As already reported, visceral involvement of MCTD is frequently mild, and often asymptomatic, at least until the damage become clinically significant. Therefore, an appropriate diagnosis requires a systematic clinical, serological and instrumental approach.

Clinical Evaluation

Clinical evaluation can allow for the objectivation of the presence of RP and/or inflammatory arthritis. The presence of puffy fingers or sclerodactyly, together with the possible presence of skin lesions can allow for the recognition of a scleroderma-phenotype, thus directing diagnostic assessment. MCTD should be considered to be a disease included among the scleroderma spectrum disorders, therefore chest auscultation can allow for the recognition of Velcro crackles typically associated with ILD. They can be present in patients without typical symptoms such as dry cough and dyspnea, therefore chest auscultation could be of great interest in clinical practice.⁴¹ An SLE-like phenotype of MCTD could be associated with malar rash, as well as decreased sound on chest auscultation, potentially associated with pleurisy. Patients could also display myalgia and proximal weakness, typically associated with a myositis phenotype.

However, clinical assessment alone is not sufficient, given that autoimmune assessment is fundamental to the recognition of anti-RNP, the hallmark of the disease, and the recognition of other potential markers associated with other conditions.

General Laboratory Evaluation

From laboratory evaluation, a complete blood count could reveal the presence of leukopenia and thrombocytopenia (both included in the sets of criteria created by Kasukawa and Tanaka^4,6), as well as the presence of hemolytic anemia. Increased levels of transaminases are possible, however, rather than being associated with hepatitis, which is very uncommon, they could be associated with myositis.³² In this case, other specific parameters are LDH (also associated with lung damage³²) and CPK, levels of which are raised in about 30% of MCTD patients.³⁹ The increased serum level of CPK is quite widely accepted as a potential criterion for MCTD classification. Hypocomplementemia and presence of proteins in the urine can also be present (42% and 10% respectively) and support an early diagnosis.³⁹ Hypergammaglobulinemia is present in about 75%, and is generally polyclonal, however a peak can also be noted: in some cases, the anti-RNP titer represents about 20% of the total amount of immunoglobulins, thus mimicking a peak.⁴²

Autoimmune Assessment

Antinuclear Antibodies (ANA) are almost always present in MCTD, a with high titer showing a coarse speckled pattern.^39,43 The presence of anti-U1RNP is required in order for the potential presence of a MCTD to be suspected, however the antibody could be also present in SLE and idiopathic inflammatory myopathies, so the full clinical picture should be considered. The antibody is included in the most common commercial kits for the assessment of Extractable Nuclear Antigens and currently represents the sole specific serological marker of MCTD. In the majority of patients, autoantibody positivity precedes the clinical onset of the disease, therefore initial assessment with ANA and ENA should be performed on all patients with suspected MCTD.⁴⁴ The presence of anti-double-stranded DNA (anti-DsDNA) is reported in about 30% of patients.³⁸ Episodic positivity for this autoantibody is possible in MCTD patients, however it should be borne in mind that this autoantibody has high specificity for active SLE, so the presence of an actual MCTD should be called into question in this case. Similar considerations can be made for ACPA, which is described in up to 50% of MCTD patients, but highly specific for RA.²⁰ The presence of anti-Ro60kDa, anti-Ro52kDa, and anti-La is reported in about 10% of patients.³⁸ Anti-La and, above all, anti-Ro60kDa are specific for Sjӧgren’s Syndrome (SS) and often associated with a significant case of sicca syndrome, allowing for the classification of MCTD patients as having secondary SS in up to 30% of cases.⁴⁵ Anti-Ro52kDa can be positive in a wide spectrum of autoimmune diseases, generally associated with myositis and ILD, therefore cases of positivity for this autoantibody should allow for appropriate assessment in patients.³² Other autoimmune parameters commonly present in MCTD are direct Coombs test, present in about up to 50% of patients and potentially associated with hemolytic anemia, and lupus anticoagulant and/or anticardiolipin antibodies, present in about 10% of patients and potentially associated with APS.³⁸ These latter parameters clearly reflect a SLE phenotype and should raise questions about the presence of an actual MCTD or SLE.

Instrumental Evaluation

As RP is one of the most common clinical signs of early MCTD, Nailfold Videocapillaroscopy (NVC) is a cheap, rapid and useful tool for the assessment of the condition. A definite scleroderma pattern was found in 45–65% of cases at first assessment.^46,47 At the early stages, the NVC pattern shows predominantly Giant Capillaries and neoangiogenesis, describing an early or active scleroderma pattern. In cases of longstanding disease, and mainly when associated with ILD, there is a predominance of avascular areas, and NVC shows a scleroderma pattern in all PAH-MCTD patients.^46–48 Interestingly, two different groups identified the possibility that immunosuppressive treatment is able to reverse NVC damage in MCTD, but not in SSc.^48,49 This fascinating topic should be studied in depth in the coming years. Despite a strong association, NVC findings are not included in any set of MCTD criteria, however the exam is very useful in the assessment of RP as primary or secondary to a condition on the scleroderma spectrum disorder and can meaningfully guide diagnosis. The presence of a late pattern should also highlight the possibility of a high risk for digital ulcers and the presence of ILD. Finally, the presence of exaggerated neoangiogenesis could suggest the presence of an underlying associated myopathic phenotype.^49,50

Figure 2 shows an NVC pattern from a female patient with MCTD.

Figure 2.

Nailfold Videocapillaroscopy in a healthy subject (A) compared with a patient with Mixed Connective Tissue Disease (B–D). The pattern is characterized by the presence of multiple Giant Capillaries and neoangiogenesis.

Pulmonary function tests (PFTs) are highly useful in the early assessment of MCTD patients. Total Lung Capacity (TLC) and Forced Vital Capacity (FVC) are usually impaired in clinically significant ILD.⁴¹ At the first assessment, abnormal PFTs are present in about a half of patients, with a slightly reduced FVC that remains stable after a decade.⁵¹ On the contrary, Diffusing Lung Capacity for Carbon Monoxide (DLCO) decreases by about 15% during follow-up, showing a correlation with the worsening of the HRCT findings.⁵² Despite the stability of FVC, about a quarter of patients showed a progressive-fibrosing phenotype of ILD, with a poor prognosis.^52,53 These data could suggest DLCO as a sensitive parameter for the recognition of ILD and its worsening in CTD patients in general, and MCTD in particular. However, DLCO is not specific for the presence of ILD, as it is potentially also impaired in the presence of PAH.⁴¹ A disproportionate relationship between FVC and DLCO values could suggest the presence of PAH. In SSc, a cut-off of 1.6 was proposed in the ratio between FVC and DLCO (both as proportion of predicted) as a clue for a possible underlying PAH.⁴¹

PFTs are clearly useful for the initial assessment of patients, but on their own, are not sufficient for an appropriate assessment of the presence of ILD. As mortality in MCTD patients is mainly due to PAH or ILD, HRCT should be performed on all patients with a significant suspicion of MCTD. As already reported, the prevalent HRCT pattern is fibrotic NSIP, followed by UIP, although episodically consolidations as features of organizing pneumonia can also be seen, suggesting a myositis phenotype in the disease.³² Studies on ILD associated with undifferentiated CTD have not proved an evolution towards MCTD, thus further supporting the idea that ILD is present in longstanding disease, or at least when the clinical picture is sufficient to reach a specific classification as MCTD.^54–56 HRCT can be also useful in evaluating possible pulmonary artery enlargement, as a clue to a possible, underlying PAH, which is relatively common in ILD-MCTD patients.⁵⁷

Appropriate screening for PAH can be carried out with a transthoracic echocardiogram, which should be considered a first-line exam in the assessment of MCTD patients. Subclinical cardiac abnormalities are present in up to 40% of patients.⁵⁸ The most common is pericardial effusion, and is useful in supporting an MCTD diagnosis, but the exam is also useful as screening for possible mitral valve prolapse, diastolic dysfunction, accelerated atherosclerosis and, above all, the possible presence of PAH.^27,58 Despite the fact that a definite diagnosis of PAH can be made only after cardiac catheterization, transthoracic echocardiogram is useful in raising suspicion of the presence of the condition, and also in follow-up for monitoring response to treatment.⁵⁹

Conclusions

MCTD is a rare condition with heterogeneous presentations, which are potentially shared with all of the other CTDs. The correct classification of these patients can be difficult due to the absence of validated classification criteria, and nuanced clinical features at the onset. In this phase, the clinical picture could resemble more common CTDs, however without sufficient features for a specific classification, and patients could be classified as Undifferentiated Connective Tissue Diseases (UCTDs). On the other hand, in longstanding disease the presence of a specific CTD is easily recognized, but the patients could satisfy the classification criteria for at least two CTDs, therefore with a potential classification as overlap syndromes. Despite the fact that, conceptually, MCTD can be considered to be an overlap disease, its classification as a distinct entity is generally accepted. The condition is actually associated with the positivity of a specific autoantibody and a genetic predisposition associated with HLA-DR4 (whereas SLE and SSc are associated with HLA DR3 and DR5 respectively).¹¹ Moreover, despite a significant proportion of MCTD patients actually develop other signs able to satisfy also the classification criteria of other CTDs, the majority of patients remains stable with a specific clinical picture including RP, inflammatory arthritis and swollen hands.⁴⁰

An early diagnosis of MCTD generally starts with the recognition of RA-like inflammatory arthritis, RP, myalgia/myositis, and rarely, trigeminal neuropathy. These signs can be present alone or in combination. A correct early diagnosis can be made starting with the presence of the anti-U1RNP antibody, which is already present in patients before clinical disease onset. To correct classify patients, serological parameters such as complete blood count, hypocomplementemia, increased level of muscle enzymes and electrophoresis can support a suspicion of MCTD. NVC, PFTs, transthoracic echocardiogram and HRCT should be performed on all patients with a significant suspicion of MCTD.

The need for widely accepted and uniform criteria for MCTD diagnosis is required to allow further advances in knowledge on this topic. The last version of classification criteria is promising, however requires to be validated also in non-Asian Cohort.

Funding Statement

This study did not benefit from any funding.

Author Contributions

All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Disclosure

Gianluca Sambataro received honoraria from Boheringer Ingelheim, outside of the submitted work. Gaetano La Rocca, Chiara Alfia Ferrara, Giuseppe Ielo, and Alessandro Libra have no conflicts of interest to declare for this work.