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. Author manuscript; available in PMC: 2023 Jul 30.
Published in final edited form as: Cell. 2022 Sep 15;185(19):3568–3587.e27. doi: 10.1016/j.cell.2022.08.019

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER

Antibodies

anti-GFP antibody Thermo Fisher Scientific Cat# A-21311; RRID: AB_221477
anti-GFP antibody Thermo Fisher Scientific Cat# A-31852; RRID: AB_162553

Bacterial and virus strains

AAV1-EF1a-DIO-GCaMP6f Stanford Viral Vector Core custom prep
AAV1-EF1a-DIO-YFP Stanford Viral Vector Core custom prep
AAV1-hSyn-YFP Stanford Viral Vector Core custom prep
AAV5-hSyn-YFP UNC Viral Vector Core custom prep
AAV8-hSyn-YFP Stanford Viral Vector Core custom prep
AAVdj-hSyn-YFP Stanford Viral Vector Core custom prep
AAVdj-hSyn-GCaMP6m Stanford Viral Vector Core custom prep
AAV8-EF1a-DIO-GCaMP6m Stanford Viral Vector Core custom prep
AAV1-EF1a-DIO-H2B-GCaMP6f Stanford Viral Vector Core custom prep
AAV1-Ef1a-DIO-ChRmine-oScarlet Stanford Viral Vector Core custom prep
AAV1-nEF-CreOnFlpOff-ChRmine-p2A-oScarlet Stanford Viral Vector Core custom prep
AAV1-nEF-CreOnFlpOff-eNpHR3.0-YFP Stanford Viral Vector Core custom prep
AAV8-Ef1a-FlpO Stanford Viral Vector Core custom prep

Chemicals, peptides, and recombinant proteins

methacryloxypropyltrimethoxysilane GE Healthcare 17-1330-01
poly-L-lysine Sigma-Aldrich P6407
Glass Bottom Plates MatTekq P12G-1.5-14-F
Micro coverglass EMS 72,226-01
OTC Fisher 23-730-571
16% PFA EMS 15710-S
PBS Gibco 70,011–044
Triton X-100 Sigma-Aldrich 93,443
OminiPur Formamide Calbiochem 75-12-7
20×SSC buffer Sigma-Aldrich S6639
dNTP mix Invitrogen 100,004,893
Tween 20 Calbiochem 655,206
SUPERase Invitrogen AM2696
RVC New England Biolabs S1402S
Salmon Sperm Invitrogen AM9680
T4 DNA ligase Thermo Scientific EL0011
Phi29 DNA polymerase Thermo Fisher Scientific EP0094
BSA New England Biolabs EP0094
BSPEG9 Thermo Fisher Scientific 21,582
Phi29 DNA polymerase Thermo Fisher Scientific EP0094
Acrylic acid NHS ester Sigma-Aldrich A8060
Methacrylic acid NHS ester Sigma-Aldrich 730,300
DMSO Sigma-Aldrich D12345
Acrylamide, 40% BioRad 161-0140
Bis-acrylamide, 2% BioRad 161-0142
Ammonium persulfate Sigma-Aldrich A3678
Tetramethylethylenediamine Sigma-Aldrich T9281
OminiPur SDS Calbiochem 7991
Shrimp Alkaline Phosphatase New England Biolabs M0371L
DAPI Molecular Probes D1306
NeuroTrace Nissl Stain Molecular Probes N-21480
Gel Slick Lonza 50,640
Proteinase K Invitrogen 25,530,049
superfrost plus slides Fisher Scientific 22-037-246
Hybrislips EMS 7,032,962
Trimethroprim (TMP) Sigma-Aldrich 92,131-1G
Heparin Sigma-Aldrich H4784-250MG
50× Denhardt’s solution Sigma-Aldrich D2532-5ML
Dextran Sulfate Sigma-Aldrich D6001
Fluorescent beads Lumafluor Red Retrobeads
Alexa 514-Labeled Hairpins Molecular Technologies B5-H1/H2-514
Alexa 555-Labeled Hairpins Molecular Technologies B2-H1/H2-555
Alexa 593-Labeled Hairpins Molecular Technologies B4-H1/H2-593
Alexa 647-Labeled Hairpins Molecular Technologies B1-H1/H2-647
Methanol Sigma-Aldrich 322,415
Dichloromethane Sigma-Aldrich 270,997
Ethyl cinnamate Sigma-Aldrich 112,372
Trypsin Sigma-Aldrich 59427C
CM-DiI Thermo Fisher Scientific V22888
DiD Thermo Fisher Scientific D7757
DID-DS Thermo Fisher Scientific D12730

Experimental models: Organisms/strains

Th-Cre (Ththm1(cre)Te Lindeberg MGI:3056580
B6; 129S-Tac1tm1.1(cre)Hze Jax 021,877
Tg(Chat-cre)GM24Gsat/Mmucd MMRRC #017269-UCD
B6.Cg-Calb1tm1.1(folA/cre)Hze/J Jax #023531
C57/Bl6 Jax #000664

Oligonucleotides

Calb1_01 IDT (for STARmap) /5Phos/ACATTAGCCAACTCTACAATTCCTATAATTATTAATGAAACATACACTAAAGATA
Calb1_02 IDT (for STARmap) /5Phos/ACATTATCAGCGTCGAAATGAAGCCAGAATTATTAATGAAACATACACTAAAGATA
Calb1_03 IDT (for STARmap) /5Phos/ACATTAGGAAATTTTCCTGCACTGGTAATTATTAATGAAACATACACTAAAGATA
Calb1_04 IDT (for STARmap) /5Phos/ACATTAATTTCATTTCCGGTGATAGCTAATTATTAATGAAACATACACTAAAGATA
Calb1_11 IDT (for STARmap) TCTTCTGTGGGTAAGACGTGTAATGTTATCTT
Calb1_12 IDT (for STARmap) CTTCCAGGTAACCACTTCCGTAATGTTATCTT
Calb1_13 IDT (for STARmap) TGATTCCCTGGAATTTAAGATAATGTTATCTT
Calb1_14 IDT (for STARmap) CTGTCCATATTGATCCACAAATAATGTTATCTT
Calb2_01 IDT (for STARmap) /5Phos/ACATTAGAGCACAATCTCCAGGTCCTAATTATTACTGAAACATACACTAAAGATA
Calb2_02 IDT (for STARmap) /5Phos/ACATTAGGTGGTGAGCTGTTGGATGTAATTATTACTGAAACATACACTAAAGATA
Calb2_03 IDT (for STARmap) /5Phos/ACATTAATCCGTAGTATGGTCTGGGTGTAATTATTACTGAAACATACACTAAAGATA
Calb2_04 IDT (for STARmap) /5Phos/ACATTAAGCCCACGTGCTGCCTGAAGCAAATTATTACTGAAACATACACTAAAGATA
Calb2_11 IDT (for STARmap) TTACACGGGGGGCTCACTGCTAATGTTATCTT
Calb2_12 IDT (for STARmap) AGGACATGACACTCTTCCTGTAATGTTATCTT
Calb2_13 IDT (for STARmap) TGCCATCTCCATTTAAGTCAAATAATGTTATCTT
Calb2_14 IDT (for STARmap) AAGCCTCCATAAACTCAGCGCTTAATGTTATCTT
Cartpt_01 IDT (for STARmap) /5Phos/ACATTACCTTTCCTCACTGCGCACTGAATTATTAGTGAAACATACACTAAAGATA
Cartpt_02 IDT (for STARmap) /5Phos/ACATTAAAGTTGCCGCCTTGGCAGCTAATTATTAGTGAAACATACACTAAAGATA
Cartpt_03 IDT (for STARmap) /5Phos/ACATTAGCGTTTACTCTTGAGCTTCTAATTATTAGTGAAACATACACTAAAGATA
Cartpt_04 IDT (for STARmap) /5Phos/ACATTAAGTAGCAGCAGGGCGGCGCCCAATTATTAGTGAAACATACACTAAAGATA
Cartpt_11 IDT (for STARmap) ACAGCTTCCCGATCCTGGCTAATGTTATCTT
Cartpt_12 IDT (for STARmap) AACATAGCGCCGGGAGCCCTAATGTTATCTT
Cartpt_13 IDT (for STARmap) ACTTCTTCTCGTAGATCGGATAATGTTATCTT
Cartpt_14 IDT (for STARmap) ACGGGCACCCAGCAAAGGTATAATGTTATCTT
Chat_01 IDT (for STARmap) /5Phos/ACATTATTCGCTCCATTCAAGCTGCAAATTATTATTGAAACATACACTAAAGATA
Chat_02 IDT (for STARmap) /5Phos/ACATTAGGACGCCATTTTGACTATCTAATTATTATTGAAACATACACTAAAGATA
Chat_03 IDT (for STARmap) /5Phos/ACATTATCTCTCATGTCAACAAGGCTAATTATTATTGAAACATACACTAAAGATA
Chat_04 IDT (for STARmap) /5Phos/ACATTAATTAATGACAACATCCAAGACAAATTATTATTGAAACATACACTAAAGATA
Chat_11 IDT (for STARmap) GGGACTTGTCATACCAACGTAATGTTATCTT
Chat_12 IDT (for STARmap) GGCAGGCGTTCATCCTCGTTAATGTTATCTT
Chat_13 IDT (for STARmap) AGGCTGCCTCGAACTACAGATAATGTTATCTT
Chat_14 IDT (for STARmap) TCACCCTCACTGAGACGGCGGTAATGTTATCTT
Elfn1_01 IDT (for STARmap) /5Phos/ACATTAAGTCTGAGACGCTCCCAGATAATTATTAATGAACCATACACTAAAGATA
Elfn1_02 IDT (for STARmap) /5Phos/ACATTACGTCGATGCAGTTGTTAATGAATTATTAATGAACCATACACTAAAGATA
Elfn1_03 IDT (for STARmap) /5Phos/ACATTATACAGGTACTCGAGCTTGCTAATTATTAATGAACCATACACTAAAGATA
Elfn1_04 IDT (for STARmap) /5Phos/ACATTACGTACTGGCGACTCTTGTCGGCAATTATTAATGAACCATACACTAAAGATA
Elfn1_11 IDT (for STARmap) CCTTGTGTCTCCGTCGGCTTAATGTTATCTT
Elfn1_12 IDT (for STARmap) GGTGGACTCGGACTTGAGGTAATGTTATCTT
Elfn1_13 IDT (for STARmap) CGATGAGGTTGGCCTGTAGTAATGTTATCTT
Elfn1_14 IDT (for STARmap) GGGGTATGAGTGCCGATGCTCTAATGTTATCTT
GAD2_01 IDT (for STARmap) /5Phos/ACATTAGGACATCAGTAACCCTCCACAATTATTACTGAACCATACACTAAAGATA
GAD2_02 IDT (for STARmap) /5Phos/ACATTACTCTAACCAGGAGAGCTGAAAATTATTACTGAACCATACACTAAAGATA
GAD2_03 IDT (for STARmap) /5Phos/ACATTAAACGCGTAGTTGACATCCCCTTAATTATTACTGAACCATACACTAAAGATA
GAD2_04 IDT (for STARmap) /5Phos/ACATTACTCCAGATTTTGCGGTTGGTCTAATTATTACTGAACCATACACTAAAGATA
GAD2_11 IDT (for STARmap) AGCTTCCACTTGTGTTTCCTAATGTTATCTT
GAD2_12 IDT (for STARmap) AGCTCTGCATCAGTCCCTCCTAATGTTATCTT
GAD2_13 IDT (for STARmap) AGCAGGTCTGTTGCGTGCATAATGTTATCTT
GAD2_14 IDT (for STARmap) TGTTTGGCAATGCGTCAAAATTTAATGTTATCTT
Gpr151_01 IDT (for STARmap) /5Phos/ACATTAAACAAATGTCAAGCTCTTGGAATTATTAGTGAACCATACACTAAAGATA
Gpr151_02 IDT (for STARmap) /5Phos/ACATTAATTGTCTTGTGCTGAAGGGAAATTATTAGTGAACCATACACTAAAGATA
Gpr151_03 IDT (for STARmap) /5Phos/ACATTATAGGATGCATGTTTGGGTCTAATTATTAGTGAACCATACACTAAAGATA
Gpr151_04 IDT (for STARmap) /5Phos/ACATTAGTTGCTGGAATTGGTGTCGGAATTATTAGTGAACCATACACTAAAGATA
Gpr151_11 IDT (for STARmap) AAGCATGCTTTGGCTACCATAATGTTATCTT
Gpr151_12 IDT (for STARmap) TGTTCCCAGGGTATAGGGTTAATGTTATCTT
Gpr151_13 IDT (for STARmap) AGACCCACGTCCGTCTGTGTTAATGTTATCTT
Gpr151_14 IDT (for STARmap) GCGAGCAAACGACTCGTTCATAATGTTATCTT
Htr5b_01 IDT (for STARmap) /5Phos/ACATTACACGCCAATCAAGATCCCGAAATTATTATTGAACCATACACTAAAGATA
Htr5b_02 IDT (for STARmap) /5Phos/ACATTAAAGGTTACTGTTGCTCGGCGAATTATTATTGAACCATACACTAAAGATA
Htr5b_03 IDT (for STARmap) /5Phos/ACATTAGGACAACTCGCTCACCAGGCTAATTATTATTGAACCATACACTAAAGATA
Htr5b_04 IDT (for STARmap) /5Phos/ACATTAATTCCATAAGAAAGTGGCAACGAATTATTATTGAACCATACACTAAAGATA
Htr5b_11 IDT (for STARmap) GGGGATCCAACAAAGCACAATAATGTTATCTT
Htr5b_12 IDT (for STARmap) CCACGAGTCTCCGCTTGTCTTAATGTTATCTT
Htr5b_13 IDT (for STARmap) CCTAGCTGCCAACGTCGCCCATAATGTTATCTT
Htr5b_14 IDT (for STARmap) AGGATAGTCACCAGAACTAGCTAATGTTATCTT
Oprm1_01 IDT (for STARmap) /5Phos/ACATTAGCATGATGAAGGCGAAGATGAATTATTAATGAAGCATACACTAAAGATA
Oprm1_02 IDT (for STARmap) /5Phos/ACATTACTTGAGTCGTAAGATCATCAGTAATTATTAATGAAGCATACACTAAAGATA
Oprm1_03 IDT (for STARmap) /5Phos/ACATTAACCCCTGCCTGTATTTTGTGGTAATTATTAATGAAGCATACACTAAAGATA
Oprm1_04 IDT (for STARmap) /5Phos/ACATTACCGTGGAGGGGTGTTCCCTAGTAATTATTAATGAAGCATACACTAAAGATA
Oprm1_11 IDT (for STARmap) ACACAGTGATGATGAGGACCTAATGTTATCTT
Oprm1_12 IDT (for STARmap) GGAGCCCGACAGCATGCGGACATAATGTTATCTT
Oprm1_13 IDT (for STARmap) AGAACGTGAGGGTGCAATCTATTAATGTTATCTT
Oprm1_14 IDT (for STARmap) GGTTAGTTCGATCCACTGTATTTAATGTTATCTT
Slc17a7_01 IDT (for STARmap) /5Phos/ACATTAGACGTAAAGAAGCGCCTCCAAATTATTACTGAAGCATACACTAAAGATA
Slc17a7_02 IDT (for STARmap) /5Phos/ACATTAACATTATGTGACGACTGCGCAATTATTACTGAAGCATACACTAAAGATA
Slc17a7_03 IDT (for STARmap) /5Phos/ACATTATTCAAAGTAGGCGGGCTGAGAGAATTATTACTGAAGCATACACTAAAGATA
Slc17a7_04 IDT (for STARmap) /5Phos/ACATTAAGGGTGGAGGTAGCCACAATGGAATTATTACTGAAGCATACACTAAAGATA
Slc17a7_11 IDT (for STARmap) ATGATGGCATAGACGGGCATTAATGTTATCTT
Slc17a7_12 IDT (for STARmap) AGCTTTCGCACGTTGGTAGTTAATGTTATCTT
Slc17a7_13 IDT (for STARmap) GCTGATCTCAAAGCCGAACACTTAATGTTATCTT
Slc17a7_14 IDT (for STARmap) GCTGCTGAAGGGATCAACATGTTAATGTTATCTT
Sst_01 IDT (for STARmap) /5Phos/ACATTACCATTGCTGGGTTCGAGTTGAATTATTAGTGAAGCATACACTAAAGATA
Sst_02 IDT (for STARmap) /5Phos/ACATTACCAGAAGAAGTTCTTGCAGCAATTATTAGTGAAGCATACACTAAAGATA
Sst_03 IDT (for STARmap) /5Phos/ACATTACCAGTTCCTGTTTCCCGGTGAATTATTAGTGAAGCATACACTAAAGATA
Sst_04 IDT (for STARmap) /5Phos/ACATTAGACGGAGTCTGGGGTCCGAGGGAATTATTAGTGAAGCATACACTAAAGATA
Sst_11 IDT (for STARmap) AGCTTTGCGTTCCCGGGGTTAATGTTATCTT
Sst_12 IDT (for STARmap) CTAACAGGATGTGAATGTCTTAATGTTATCTT
Sst_13 IDT (for STARmap) AGCTCTGCCAAGAAGTACTTTAATGTTATCTT
Sst_14 IDT (for STARmap) CGCCAGAGACTTCTGCAGAAATAATGTTATCTT
Sstr4_01 IDT (for STARmap) /5Phos/ACATTAAAGCACTGCGCGACACAGCAAATTATTATTGAAGCATACACTAAAGATA
Sstr4_02 IDT (for STARmap) /5Phos/ACATTAGCACGAGCCTAGTGATCTTCAATTATTATTGAAGCATACACTAAAGATA
Sstr4_03 IDT (for STARmap) /5Phos/ACATTAGATGACCAGGGCGTTTCCCAAATTATTATTGAAGCATACACTAAAGATA
Sstr4_04 IDT (for STARmap) /5Phos/ACATTAAGAACCGGGAGTAGGAAGCCCAAATTATTATTGAAGCATACACTAAAGATA
Sstr4_11 IDT (for STARmap) ATGTTCAGGCCGTCCACGCTAATGTTATCTT
Sstr4_12 IDT (for STARmap) AAAGACGGTCACCACCATTTAATGTTATCTT
Sstr4_13 IDT (for STARmap) TGGCATAGCGTAGGATCACGTAATGTTATCTT
Sstr4_14 IDT (for STARmap) AGCAGGTAGCATAATCCGATGGTAATGTTATCTT
Syt10_01 IDT (for STARmap) /5Phos/ACATTAGTCGTCTTTTCTGTTGCCCTAATTATTAATGAATCATACACTAAAGATA
Syt10_02 IDT (for STARmap) /5Phos/ACATTATTCGACACACGCCTATGACCTAATTATTAATGAATCATACACTAAAGATA
Syt10_03 IDT (for STARmap) /5Phos/ACATTATTTCCATACTGTGGCTTCCCTAATTATTAATGAATCATACACTAAAGATA
Syt10_04 IDT (for STARmap) /5Phos/ACATTACTCGTTCCAATGGTCTCTCCCAAATTATTAATGAATCATACACTAAAGATA
Syt10_11 IDT (for STARmap) AGTTTCCCACAGGTCTTGATAATGTTATCTT
Syt10_12 IDT (for STARmap) AGACCCTCAGCGTCTAGTCCTAATGTTATCTT
Syt10_13 IDT (for STARmap) TCTGTGGTAGCACAGTGGATATAATGTTATCTT
Syt10_14 IDT (for STARmap) TGGCTTTCGGTGATAGGCCAGCTAATGTTATCTT
Tac1_01 IDT (for STARmap) /5Phos/ACATTAGCGATTCTCTGCAGAAGATGAATTATTACTGAATCATACACTAAAGATA
Tac1_02 IDT (for STARmap) /5Phos/ACATTACAAAGAACTGCTGAG GCTTGAATTATTACTGAATCATACACTAAAGATA
Tac1_03 IDT (for STARmap) /5Phos/ACATTAATCGCGCTTCTTTCATAAGCAATTATTACTGAATCATACACTAAAGATA
Tac1_04 IDT (for STARmap) /5Phos/ACATTACCCATTAGTCCAACAAAGGAATAATTATTACTGAATCATACACTAAAGATA
Tac1_11 IDT (for STARmap) GCTGAGGCTTGGGTCTTCGTAATGTTATCTT
Tac1_12 IDT (for STARmap) ATCCCGCTTGCCCATTAATTAATGTTATCTT
Tac1_13 IDT (for STARmap) ACGTCTTCTTTCGTAGTTCTTAATGTTATCTT
Tac1_14 IDT (for STARmap) GCCACAGAATTTAAAGCTCTTTTAATGTTATCTT
Tac2_01 IDT (for STARmap) /5Phos/ACATTAAGCCGCAAACAGCATGGCGCAATTATTAGTGAATCATACACTAAAGATA
Tac2_02 IDT (for STARmap) /5Phos/ACATTACGGTGGGAGTGTCTGGTTGGCAATTATTAGTGAATCATACACTAAAGATA
Tac2_03 IDT (for STARmap) /5Phos/ACATTATGTCACGTTTCTGTGGAAGTGAATTATTAGTGAATCATACACTAAAGATA
Tac2_04 IDT (for STARmap) /5Phos/ACATTATCTCCGAAGCAGGGACGGAGGCAATTATTAGTGAATCATACACTAAAGATA
Tac2_11 IDT (for STARmap) AGCCAAGCTGAGGGCGAGGATAATGTTATCTT
Tac2_12 IDT (for STARmap) GGGGTGTTCTCTTCAACCACTAATGTTATCTT
Tac2_13 IDT (for STARmap) AGTCCCACAAAGAAGTCGTGTAATGTTATCTT
Tac2_14 IDT (for STARmap) AGAGACAGGGCGGCTGTCGTAGTAATGTTATCTT
Th_01 IDT (for STARmap) /5Phos/ACATTAGAAGTGAGACACATCCTCCAAATTATTATTGAATCATACACTAAAGATA
Th_02 IDT (for STARmap) /5Phos/ACATTACTCGAATACCACAGCCTCCAAATTATTATTGAATCATACACTAAAGATA
Th_03 IDT (for STARmap) /5Phos/ACATTAGGCTTCAAATGTCTCAAACACTAATTATTATTGAATCATACACTAAAGATA
Th_04 IDT (for STARmap) /5Phos/ACATTAGAGGCATAGTTCCTGAGCTTGTAATTATTATTGAATCATACACTAAAGATA
Th_11 IDT (for STARmap) AAGCCAGTCCGTTCCTTCATAATGTTATCTT
Th_12 IDT (for STARmap) ACAGCATTTCCATCCCTCTTAATGTTATCTT
Th_13 IDT (for STARmap) CCGGGTCTCTAAGTGGTGGATTTAATGTTATCTT
Th_14 IDT (for STARmap) ACAGAGAATGGGCGCTGGATACTAATGTTATCTT
Mus-Calb1-B4P1 IDT (for ISH) cactctcaaactagccgctgcaccacgatggcagaatcccacctgcagtcat
Mus-Calb1-B4P2 IDT (for ISH) agaaaagtagatatcgtgaatcattatagccgaaggactctaataaaaattt
Mus-Calb1-B4P3 IDT (for ISH) aatagtaccaagtttggcattcttactaatagtagtagaacaaatcctataa
Mus-Calb1-B4P4 IDT (for ISH) cgccgcgcccagctcagcctgcgcagccctctcgcccgaggttcgcgctccg
Mus-Calb1-B4P5 IDT (for ISH) aagactgtggatgatacaaaactagcagagtacacagacctcatgctgaaac
Mus-Calb1-B4P6 IDT (for ISH) ccagtgcaggaaaatttccttcttaaattccagggaatcaaaatgtgtggga
Mus-Calb1-B4P7 IDT (for ISH) ggagggaagctgtaccgaacagaccttgctcttattctttctgctggagaca
Mus-Calb1-B4P8 IDT (for ISH) tagagttggtgaccacaaccacttgctagtgatacattgtatctaaaaccat
Mus-Calb1-B4P9 IDT (for ISH) aaatatcaacagttaattatggctttattctgaaacgatctccctagagatt
Mus-Calb1-B4P10 IDT (for ISH) atgcctatatttccaagaagtctactgccagagagtatgaccatagcccatt
Mus-Calb1-B4P11 IDT (for ISH) ctaaattattttcatgtgttccagatgacaattattctagtaaactgctgtt
Mus-Calb1-B4P12 IDT (for ISH) tatttcatcaaaacttgtgtattctgtggattctatggttcatattgagatc
Mus-Calb1-B4P13 IDT (for ISH) aggtttgggtcaggttggatttaagcactttttttcaattgttgttcataaa
Mus-Calb1-B4P14 IDT (for ISH) atttgtttctaccccaaagtgttaattgtcatgtaatctgttatcaattagg
Mus-Calb1-B4P15 IDT (for ISH) gcgcgaaagaaggctggattggagctatcaccggaaatgaaatcctttgtgg
Mus-Calb1-B4P16 IDT (for ISH) caatatggacagagagatgatggaaaaataggaattgtagagttggctcacg
Mus-Calb1-B4P17 IDT (for ISH) ttacccacagaagagaatttcttgctgctctttcgatgccagcaactgaagt
Mus-Calb1-B4P18 IDT (for ISH) atcgaaaccgaggaacttaagaactttctaaaggacctactagagaaagcaa
Mus-Calb1-B4P19 IDT (for ISH) tttgattcaaataatgacggaaagctggaactgacagagatggccaggttac
Mus-Calb1-B4P20 IDT (for ISH) gagttcaataaggcttttgagttatatgatcaggatggcaacggatacatag
Mus-ChAT-B5P1 IDT (for ISH) gcgtccaacgaggatgaacgcctgcctccaatcggcctgctgacgtcagacg
Mus-ChAT-B5P2 IDT (for ISH) gctaggatgcctatcctggaaaaggtccccccaaagatgcctgtacaagctt
Mus-ChAT-B5P3 IDT (for ISH) gaatactggctgaatgacatgtatctaaacaaccgcctggccctgccagtca
Mus-ChAT-B5P4 IDT (for ISH) cgccgtctcagtgagggtgatctgttcactcagttgagaaagatagtcaaaa
Mus-ChAT-B5P5 IDT (for ISH) atgagagacctctgtagttcgaggcagcctgctgaaggcaagccaccaacag
Mus-ChAT-B5P6 IDT (for ISH) aaggaaagagctagaggcccaaccaagccaagcaatcttgactactcccact
Mus-ChAT-B5P7 IDT (for ISH) ggtctttagaaacttaacctttctgcttctttcccagcaacacccagtggtg
Mus-ChAT-B5P8 IDT (for ISH) gcgtaacagcccaggagagcaggtcggcagctctgctactctggattaagaa
Mus-ChAT-B5P9 IDT (for ISH) agctgtgaggaggtgctggacttacctaagttgccagtgcccccactgcagc
Mus-ChAT-B5P10 IDT (for ISH) aggaagagccaggccattgtgaagcggtttggggcccctggtggcctgggtg
Mus-ChAT-B5P11 IDT (for ISH) tctagccctgctgtgatctttgctcggcagcacttccaagacaccaatgacc
Mus-ChAT-B5P12 IDT (for ISH) ctaaggtttgcagccagcctcatctctggtgtgcttagctacaaggctctgc
Mus-ChAT-B5P13 IDT (for ISH) ggcactggagacctcagtgacacacacagggccctccagctccttcatggtg
Mus-ChAT-B5P14 IDT (for ISH) ggctgcagcttgaatggagcgaatcgttggtatgacaagtccctgcagtttg
Mus-ChAT-B5P15 IDT (for ISH) aagaagctcgtccgagctgactcagtgagtgaactccctgctcccagaaggc
Mus-ChAT-B5P16 IDT (for ISH) gccctccagctggcttactacaggctttaccagaggctggtgcccacctatg
Mus-ChAT-B5P17 IDT (for ISH) tacacagtcatggccataaccggcatggccattgacaaccatcttctggcac
Mus-ChAT-B5P18 IDT (for ISH) tataacccccagcctgaggccatcaccttctgcatctccagctttcacggct
Mus-ChAT-B5P19 IDT (for ISH) gcttgttgctgctcccctatccttgggggctcacatgaagctggcatgttaa
Mus-ChAT-B5P20 IDT (for ISH) atgagacccagcctggcttggaagcagcctgggtgggctgggagctccctct
Mus-Tac1-B2P1 IDT (for ISH) gtgcgcacctgcggagcatccccgcggtctgaccgcaaaatcgaacatgaaa
Mus-Tac1-B2P2 IDT (for ISH) aatcgatgccaacgatgatctaaattattggtccgactggtccgacagtgac
Mus-Tac1-B2P3 IDT (for ISH) gatcaaggaggcaatgccggagccctttgagcatcttctgcagagaatcgcc
Mus-Tac1-B2P4 IDT (for ISH) tttaaattctgtggcttatgaaagaagcgcgatgcagaactacgaaagaaga
Mus-Tac1-B2P5 IDT (for ISH) ataatgtactgagacttcggtatttgactctatttgtatcctagcagcatgt
Mus-Tac1-B2P6 IDT (for ISH) ctctcacaaaaggcataaaacagattcctttgttggactaatgggcaaaaga
Mus-Tac1-B2P7 IDT (for ISH) taaataaacccctgaacgcactatctattcatcttcatctgtgtcagtgagc
Mus-Tac1-B2P8 IDT (for ISH) gtaatttcagcaaagcacagtgatgaaggagctgtccaagcttggcagtgac
Mus-Tac1-B2P9 IDT (for ISH) gcagagactcctgtgcgtctctctcacgctacccctggttctgctttcatgc
Mus-Tac1-B2P10 IDT (for ISH) cctgtttcgtgactatatagagatgttttgaaaaagtttcaatgtaattctc
Mus-Tac1-B2P11 IDT (for ISH) aagacccaagcctcagcagttctttggattaatgggcaagcgggatgctgat
Mus-Tac1-B2P12 IDT (for ISH) cggacctgctccgctcctgcaccgcggccaaggagagcaaagagcgcccagc
Mus-Tac1-B2P13 IDT (for ISH) cctcgtggccgtggcggtcttttttctcgtttccactcaactgtttgcagag
Mus-Tac1-B2P14 IDT (for ISH) ctcagttgaaaaacaagtggccctgttaaaggctctttatggacatggccag
Mus-Tac1-B2P15 IDT (for ISH) tgaacggtaaaataaaatgtgcgctatgaggaatgattatttatttaataac
Mus-Tac1-B2P16 IDT (for ISH) ccaataagccttgtaattctaatgtggtgacctccccagaagtagaaattag
Mus-Tac1-B2P17 IDT (for ISH) atgttgttgtgagtgaaaaactcaaaaaagaagtgtttattttttcatattg
Mus-Tac1-B2P18 IDT (for ISH) agtctccaaagaaaggacccttctgtgagccagcgcaggcagctgctgctgg
Mus-Tac1-B2P19 IDT (for ISH) gtcttcagtcattgtatgatgtgttgtgatagctaccattttaaataaaaga
Mus-Th-B1P1 IDT (for ISH) ctcctcagttctgtgcgtcgggtgtctgacgatgtgcgcagtgccagagagg
Mus-Th-B1P2 IDT (for ISH) gtactggacagtcctcacaccatccggcgctccttagagggggtccaggatg
Mus-Th-B1P3 IDT (for ISH) tcagagcaggataccaagcaggccgaggctgtcacgtccccaaggttcattg
Mus-Th-B1P4 IDT (for ISH) cggcggcagagtctcatcgaggatgcccgcaaggagcgggaggcagcagcag
Mus-Th-B1P5 IDT (for ISH) gcagcagcggctgcggtagcctccgcggaacctgggaacccattggaggctg
Mus-Th-B1P6 IDT (for ISH) gtattcgaggagagggatggaaatgctgttctcaacctgctcttctccttga
Mus-Th-B1P7 IDT (for ISH) gccaaaatccaccacttagagacccggcctgcccagaggccactggcaggaa
Mus-Th-B1P8 IDT (for ISH) ccccacctggagtactttgtgcgcttcgaggtgcccagtggcgacctggctg
Mus-Th-B1P9 IDT (for ISH) aaggttccctggttcccaaggaaagtgtcagagttggataagtgtcaccacc
Mus-Th-B1P10 IDT (for ISH) gaggtatacgccacgctgaagggcctctatgctacccatgcctgccgggaac
Mus-Th-B1P11 IDT (for ISH) cgacccgtggccggtctactgtctgcccgtgattttctggccagtctggcct
Mus-Th-B1P12 IDT (for ISH) cgtgtgtttcagtgcacacagtacatccgtcatgcctcctcacctatgcact
Mus-Th-B1P13 IDT (for ISH) tatggagagctcctgcactccctgtcagaggagcccgaggtccgggcctttg
Mus-Th-B1P14 IDT (for ISH) gtcaccaagtttgaccctgacctggacctggaccatccgggcttctctgacc
Mus-Th-B1P15 IDT (for ISH) gcgtatcgccagcgccggaagctgattgcagagattgccttccaatacaagc
Mus-Th-B1P16 IDT (for ISH) ctggaggctttccagcttctggaacggtactgtggctaccgagaggacagca
Mus-Th-B1P17 IDT (for ISH) Cccgagccagactgctgccacgagctgctgggacacgtacccatgttggctg
Mus-Th-B1P18 IDT (for ISH) ctgtgtaaacagaatggggagctgaaggcttacggtgcagggctgctgtctt
Mus-Th-B1P19 IDT (for ISH) ccagacacagcagccgtgcagccctaccaagatcaaacctaccagccggtgt
Mus-Th-B1P20 IDT (for ISH) cgtatccagcgcccattctctgtgaagtttgacccgtacaccctggccattg

Software and algorithms

MedPC-IV MedAssociates https://www.med-associates.com/med-pc-v/
Viewer3 BioObserve http://www.biobserve.com/behavioralresearch/
FIJI OpenSource https://ImageJ.net/ImageJ
MATLAB Mathworks https://www.mathworks.com/products/matlab.html
DeepLabCut Mathis et al. (2018) https://github.com/DeepLabCut
CNMF Pnevmatikakis et al. (2016) https://github.com/epnev/ca_source_extraction
SpikeGLX (and associated command-line tools: CatGT, TPrime, and C_Waves) Bill Karsh (Janelia Research Campus) https://billkarsh.github.io/SpikeGLX
Extracellular electrophysiology data processing pipeline Jennifer Colonell (Janelia Research Campus) https://github.com/jenniferColonell/ecephys_spike_sorting
Kilosort 2.5 Pachitariu et al., (2016) https://github.com/MouseLand/Kilosort
Phy Cyrille Rossant (International Brain Laboratory) https://github.com/cortex-lab/phy
Shield-2018 Swaney et al., (2019) https://hub.docker.com/r/chunglabmit/shield-2018 https://github.com/chunglabmit/shield-2018
Elastix Klein et al., (2010) https://elastix.lumc.nl
Lasagna OpenSerialSection (University College London) https://github.com/SainsburyWellcomeCentre/lasagna
Atlas Electrophysiology International Brain Laboratory; Liu et al., (2021) https://github.com/int-brain-lab/iblapps/tree/master/atlaselectrophysiology
AllenCCF University College London https://github.com/cortex-lab/allenCCF
Computation-through-dynamics (LFADS and fixed point analysis) Pandarinath et al. (2018b); Sussillo and Barak (2013) https://github.com/google-research/computation-thru-dynamics

Deposited data

STARmap data NeMO Archive asset.nemoarchive.org/dat-9ACQ8G2
Electrophysiology data DANDI Archive N/A