30% probe wash buffer | For 40 mL of solution |
---|---|
Fill up to 40 mL with ultrapure H2O | |
30% formamide | 12 mL formamide |
5× sodium chloride sodium citrate (SSC) | 10 mL of 20× SSC |
9 mM citric acid (pH 6.0) | 360 μL 1 M citric acid, pH 6.0 |
0.1% Tween 20 | 400 μL of 10% Tween 20 |
50 μg/mL heparin | 200 μL of 10 mg/mL heparin |