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. Author manuscript; available in PMC: 2023 Jul 30.
Published in final edited form as: Cell. 2022 Sep 15;185(19):3568–3587.e27. doi: 10.1016/j.cell.2022.08.019
30% probe wash buffer For 40 mL of solution

Fill up to 40 mL with ultrapure H2O
30% formamide 12 mL formamide
5× sodium chloride sodium citrate (SSC) 10 mL of 20× SSC
9 mM citric acid (pH 6.0) 360 μL 1 M citric acid, pH 6.0
0.1% Tween 20 400 μL of 10% Tween 20
50 μg/mL heparin 200 μL of 10 mg/mL heparin