Amplification buffer | For 40 mL of solution |
---|---|
Fill up to 40 mL with ultrapure H2O | |
5× sodium chloride sodium citrate (SSC) | 10 mL of 20× SSC |
0.1% Tween 20 | 400 μL of 10% Tween 20 |
10% dextran sulfate | 8 mL of 50% dextran sulfate |
Amplification buffer | For 40 mL of solution |
---|---|
Fill up to 40 mL with ultrapure H2O | |
5× sodium chloride sodium citrate (SSC) | 10 mL of 20× SSC |
0.1% Tween 20 | 400 μL of 10% Tween 20 |
10% dextran sulfate | 8 mL of 50% dextran sulfate |