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. 2023 Jul 11;26(8):107333. doi: 10.1016/j.isci.2023.107333

Figure 4.

Figure 4

PAK1 inhibition partially reverses the reduction in surface expression of junction proteins during OGD/R

(A) Immunoblots of occludin and ve-cadherin in bEnd.3 cells after OGD 2 h and reoxygenation 3 h. The biotinylated proteins were shown as surface protein and the proteins in whole cell lysates were shown as total protein. GAPDH was used as a loading control.

(B) The statistical analysis of surface/total occludin and ve-cadherin. Relative levels are normalized to respective control groups and expressed as mean ± SEM (n = 4). ∗p < 0.05, ∗∗∗p < 0.001 versus control groups; #p < 0.05, ##p < 0.01, ###p < 0.001 versus OGD+vehicle group. One-way ANOVA with Sidak’s post hoc test.

(C) The statistical analysis of total occludin and ve-cadherin. Relative levels are normalized to respective control groups and expressed as mean ± SEM (n = 4).

(D) Immunofluorescence of ZO-1 in bEnd.3 cells with different treatment (vehicle, FRAX486, IPA-3) after OGD/R. White arrow shows internalized ZO-1. Scale bar = 20 μm.